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Lasers in Medical Science

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01.01.2013 | Original Article

Determination of the effect of Nd:YAG laser irradiation through dentinal tubules on several oral pathogens

verfasst von: Murat Maden, Güliz Görgül, M. Nedim Sultan, Gülçin Akça, Özgür Er

Erschienen in: Lasers in Medical Science | Ausgabe 1/2013

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Abstract

The effects of Nd:YAG laser irradiations at different power settings on several oral pathogens were evaluated. A total of 252 dentin samples were divided into seven groups consisting of 36 dentin specimens each. In each group, 9 of the 36 specimens were used as controls, thereby including a control in every group. The remaining 27 specimens were divided into three subgroups consisting of nine specimens according to different Nd:YAG laser settings (1.5, 1.8, and 2 W). Each group was inoculated on the nonpulpal side with one of the following microorganisms: Candida glabrata, Candida tropicalis, Candida krusei, Candida sake, Candida lusitaniae, Candida kefyr, and Rhodotorula mucilaginosa. The following irradiation procedure was used: the specimens were irradiated on the bacteria-free side (the side consisting of the pulpal wall) using contact mode under the constant scanning movement of the optical fiber at an angle of 10°. One lasing cycle consisted of four irradiation cycles of 10 s each, with 15-s intervals in between each irradiation cycle. The remainder of the controls and the lased specimens of each group were prepared for the microbiological investigation. After incubation for 24 h at 37 °C, the colonies were counted, and the total number of surviving microorganisms was statistically assessed. Microorganisms irradiated with Nd:YAG laser at power settings 2 W, 15 pps did not survive. Although there was a significant reduction of microorganisms at 1.5 and 1.8 W, when comparing Nd:YAG laser irradiation with the control group, sterilization did not occur.

Siqueira JF, Sen BH (2004) Fungi in endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 97:632–641PubMedCrossRef

Egan MV, Spratt DA, Ng YL, Lam JM, Moles DR, Gulabivala K (2002) Prevalance of yeasts in saliva and root canals of teeth associated with apical periodontits. Int Endod J 35:321–329PubMedCrossRef

Sundqvist G, Figdor D, Persson S, Sjögren U (1998) Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:86–93PubMedCrossRef

Waltimo T, Sirén E, Ørstavik D, Haapasolo M (1999) Susceptibility of oral Candida species to calcium hydroxide in vitro. Int Endod J 32:94–98PubMedCrossRef

Baumgartner JC, Watts CM, Xia T (2000) Occurrence of Candida albicans in infections of endodontic origin. J Endod 26:695–698PubMedCrossRef

Bagg J, Silverwood RW (1986) Coagglutination reactions between Candida albicans and oral bacteria. J Med Microbiol 22:165–169PubMedCrossRef

Jenkinson HF, Lala HC, Shepherd MG (1990) Coaggregation of Streptococcus sanguis and other Streptococci with Candida albicans. Inf Immun 58:1429–1436

Şen BH, Pişkin B, Demirci T (1995) Observation of bacteria and fungi in infected root canals and dentinal tubules by SEM. Endod Dent Traumatol 11:6–9PubMedCrossRef

Waltimo TM, Ørstavik D, Sirén EK, Haapasalo MP (2000) In vitro yeast infection of human dentin. J Endod 26:207–209PubMedCrossRef

10.

Frentzen M, Koort HJ (1990) Lasers in dentistry: new possibilities with advancing laser technology. Int Dent J 40:323–332PubMed

11.

Piccolomini R, D’Arcangelo C, D’Ercole S, Catamo G, Schiaffino G, De Fazio P (2002) Bacteriologic evaluation of the effect of Nd:YAG laser irradiation in experimental infected root canals. J Endod 28:176–278CrossRef

12.

Mashanov J, Ørstavik D, Yamauchi J, Peetiette M, Trope M (1995) Nd:YAG laser irradiation in root canal disinfections. Endod Dent Traumatol 11:220–224CrossRef

13.

Fegan SE, Steiman HR (1995) Comparative evaluation of the antibacterial effects of intracanal Nd:YAG laser irradiation: an in vitro study. J Endod 21:415–417PubMedCrossRef

14.

Folwaczny M, Mehl A, Jordan C, Hickel R (2002) Antibacterial effects of pulsed Nd:YAG Laser radiation at different energy settings in root canals. J Endod 28:24–29PubMedCrossRef

15.

Zhang C, Kimura Y, Matsumoto K, Harashima T, Zhou H (1998) Effects of pulsed Nd:YAG laser irradiations on root canal wall dentin with different laser initiators. J Endod 24:352–353PubMedCrossRef

16.

Gutknecht N, Franzen R, Schippers M, Lampert F (2004) Bactericidal effect of a 980-nm diode laser in the root canal wall dentin of bovine teeth. J Clin Laser Med Surg 22:9–13PubMedCrossRef

17.

Gouw-Soares S, Gutknecht N, Conrads G, Lampert F, Matson E, Eduardo CP (2000) The bactericidal effect of Ho:YAG laser irradiation within contaminated root dentinal samples. J Clin Laser Med Surg 18:81–87PubMed

18.

Gutknecht N, Nuebler-Moritz M, Burghardt SF, Lampert F (1997) The efficiency of root canal disinfection using a holmium:yttrium-aluminum-garnet laser in vitro. J Clin Laser Med Surg 15:75–78PubMed

19.

Myers TD (1991) Laser in dentistry. J Am Dent Assoc 122:46–50PubMed

20.

Pick RM (1993) Using lasers in clinical dental practice. J Am Dent Assoc 124:37–47PubMed

21.

Misserendino LJ, Pick RM (1995) Laser interaction with biologic tissues in laser in dentistry. Quintessence, Chicago, pp 57–61, 67

22.

Takeda FH, Harashima T, Kimura Y, Matsumoto K (1999) A comparative study of the removal of smear layer by three endodontic irrigants and two types of laser. Int Endod J 32:32–39PubMedCrossRef

23.

Levy G (1992) Cleaning and shaping the root canal with a Nd:YAG laser beam: a comparative study. J Endod 18:123–127PubMedCrossRef

24.

Prati C, Selighini M, Ferrieri P, Mongiorgi R (1994) Scanning electron microscopic evaluation of different endodontic procedures on dentin morphology of human teeth. J Endod 20:174–179PubMedCrossRef

25.

Byström A, Sundqvist G (1985) The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J 18:35–40PubMedCrossRef

26.

Gutknecht N, Moritz A, Conrads G, Sievert T, Lampert F (1996) Bactericidal effect of the Nd:YAG laser in in vitro root canals. J Clin Laser Med Surg 14:77–80PubMed

27.

Gutknecht N, Kaiser F, Hassan A, Lampert F (1996) Long-term clinical evaluation of endodontically treated teeth by Nd:YAG lasers. J Clin Laser Med Surg 14:7–11PubMed

28.

Moritz A, Jakolitsch S, Goharkhay K, Schoop U, Kluger W, Mallinger R, Sperr W, Georgopoulos A (2000) Morphologic changes correlating to different sensitivities of Escherichia coli and Enterococcus faecalis to Nd:YAG laser irradiation through dentin. Lasers Surg Med 26:250–261PubMedCrossRef

29.

Lana MA, Ribeiro-Sobrinho AP, Stehling R, Garcia GD, Silva BK, Hamdan JS et al (2001) Microorganisms isolated from root canals presenting necrotic pulp and their drug susceptibility in vitro. Oral Microbiol Immunol 16:100–105PubMedCrossRef

30.

Waltimo TMT, Sen BH, Meurman JH, Ørstavik D, Haapasalo MPP (2003) Yeasts in apical periodontitis. Crit Rev Oral Biol Med 14:128–137PubMedCrossRef

31.

Kirshner SS, Schwarz P, Schliephake H, Konietschke F, Brunner E, Zepelin MB (2009) Decontamination efficacy of erbium:yttrium-aluminium-garnet and diode laser light on oral Candida albicans isolates of a 5-day in vitro biofilm model. Lasers Med Sci 24:313–320CrossRef

32.

Meire MA, Coenye T, Nelis HJ, De Moor RJG (2011) In vitro inactivation of endodontic pathogens with Nd:Yag and Er:YAG lasers. Lasers Med Sci. doi:10.1007/s10103-011-0940-z

33.

Alberth M, Majoros L, Kovalecz G, Borbas E, Szegedi I, Marton IJ, Kıss C (2006) Significance of oral Candida infections in children with cancer. Pathol Oncol Res 12:237–241PubMedCrossRef

34.

Hasan F, Xess I, Wang X, Jain N, Fries BC (2009) Biofilm formation in clinical Candida isolates and its association with virulance. Microbes Infect 11:753–761PubMedCrossRef

35.

Miranda TT, Vianna CR, Rodrigues L, Monteiro AS, Rosa CA, Corrêa A (2009) Diversity and frequency of yeasts from the dorsum of the tongue and necrotic root canals associated with primary apical periodontitis. Int Endod J 42:839–844PubMedCrossRef

36.

McDonald JB, Hare GC, Wood AWS (1957) The bacteriological status of the pulp chambers in intact teeth found to be non-vital following trauma. Oral Surg Oral Med Oral Path 10:318–322CrossRef

37.

Waltimo TMT, Sirén EK, Torkko HLK, Olsen I, Phaapasalo MP (1997) Fungi in therapy-resistant apical periodontits. Int Endod J 30:96–101PubMed

38.

Şen HB, Safavi EK, Spönberg LSW (1999) Antifungal effects of sodium hypochlorite and clorhexidine in root canals. J Endod 25:235–238PubMedCrossRef

39.

Perin FM, Franca SC, Silva-Sousa YT, Alfredo E, Saquy PC, Estrala C, Sousa-Neto MD (2004) Evaluation of the antimicrobial effect of Er:YAG laser irradiation versus 1% sodium hypochlorite irrigation for root canal disinfection. Aus Endod J 30:20–22CrossRef

40.

Vezzani MS, Pietro R, Silva-Sousa YT, Brugnera-Junior A, Sousa-Neto MD (2006) Disinfection of root canals using Er:YAG laser at different frequencies. Photomed Laser Surg 24:499–502PubMedCrossRef

41.

Onay EO, Alikaya C, Seker E (2010) Evaluation of antifungal efficacy of erbium, chromium: yttrium-scandium-gallium-garnet laser against Candida albicans. Photomed Laser Surg 28:S73–S78PubMedCrossRef

42.

Gutknecht N, van Gogswaardt D, Conrads G, Apel C, Schubert C, Lampert F (2000) Diode laser radiation and its bactericidal effect in root canal wall dentin. J Clin Laser Med Surg 18:57–60PubMed

43.

Franzen R, Gutknecht N, Falken S, Heussen N, Meister J (2011) Bactericidal effect of a Nd:YAG laser on Enterococcus faecalis at pulse durations of 15 and 25 ms in dentine depths of 500 and 1,000 μm. Lasers Med Sci 26:95–101PubMedCrossRef

44.

Klinke T, Klimm W, Gutknecht N (1997) Antibacterial effects of Nd:YAG laser irradiation within root canal dentin. J Clin Laser Med Surg 15:29–31PubMed

45.

Schoop U, Kluger W, Moritz A, Nedjelik N, Georgopoulos A, Sperr W (2004) Bactericidal effect of different laser systems in the deep layers of dentin. Lasers Surg Med 35:111–116PubMedCrossRef

Titel: Determination of the effect of Nd:YAG laser irradiation through dentinal tubules on several oral pathogens
verfasst von: Murat Maden
Güliz Görgül
M. Nedim Sultan
Gülçin Akça
Özgür Er
Publikationsdatum: 01.01.2013
Verlag: Springer-Verlag
Erschienen in: Lasers in Medical Science / Ausgabe 1/2013
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-012-1150-z

Springer Medizin

Abstract

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