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Archives of Virology

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13.10.2016 | Brief Report

Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus

verfasst von: Mohammad Amin Almasi, Galavizh Almasi

Erschienen in: Archives of Virology | Ausgabe 2/2017

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Abstract

Sugar beet can be infected by many different viruses that can reduce yield; beet necrotic yellow vein virus (BNYVV) is one of the most economically important viruses of this crop plant. This report describes a new reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for identification of BNYVV. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection (without RNA extraction) of BNYVV was developed here and compared with DAS-ELISA and RT-LAMP assays. Our results show that the IC-RT-LAMP assay is a highly reliable alternative assay for identification of BNYVV.

Kutluk Yilmaz ND (2016) Identification of strain types of some beet necrotic yellow vein virus isolates determined in Northern and Central Parts of Turkey. Eurasian J Soil Sci 5:241–248CrossRef

Mahmoudi SB, Ghanbari M, Amiri R, Darabi S, Kakuei nejad M, Aghaee zadeh M, Hasani M (2012) Relative levels of beet necrotic yellow vein virus in susceptible to resistant genotypes of sugar beets during growing season. J Sugar Beet 28:1–6

Mehrvar M, Valizadeh J, Koenig R, Bragard CG (2009) Iranian beet necrotic yellow vein virus (BNYVV): pronounced diversity of the p25 coding region in A-type BNYVV and identification of P-type BNYVV lacking a fifth RNA species. Arch Virol 154:501–506CrossRefPubMed

Arif M, Khan W, Shafi A, Kamranullah (2015) Detection of beet necrotic yellow vein virus in Pakistan using bait-plant bioassay, ELISA and RT-PCR. Afr J Biotechnol 14:3206–3215CrossRef

Erhardt M, Dunoyer P, Guilley H, Richards K, Jonard G, Bouzoubaa S (2001) Beet necrotic yellow vein virus particles localize to mitochondria during infection. Virology 286:256–262CrossRefPubMed

Meunier A, Schmit JF, Stas A, Kutluk N, Bragard C (2003) Multiplex reverse transcription-PCR for simultaneous detection of beet necrotic yellow vein virus, beet soilborne virus, and beet virus Q and their vector Polymyxa betae KESKIN on sugar beet. Appl Environ Microbiol 69:2356–2360CrossRefPubMedPubMedCentral

Schirmer A, Link D, Cognat V, Moury B, Beuve M, Meunier A, Bragard C, Gilmer D, Lemaire O (2005) Phylogenetic analysis of isolates of beet necrotic yellow vein virus collected worldwide. J Gen Virol 86:2897–2911CrossRefPubMed

Harju VA, Skelton A, Clover GR, Ratti C, Boonham N, Henry CM, Mumford RA (2005) The use of real-time RT-PCR (TaqMan) and post-ELISA virus release for the detection of beet necrotic yellow vein virus types containing RNA 5 and its comparison with conventional RT-PCR. J Virol Methods 123:73–80CrossRefPubMed

Ghorbani A, Izadpanah K, Manzari F, Roumi V (2013) A simple method for detection of Polymyxa betae and beet necrotic yellow vein virus in Soil. J Plant Pathol 95:533–537

10.

Acosta-Leal R, Bryan BK, Rush CM (2010) Host effect on the genetic diversification of beet necrotic yellow vein virus single-plant populations. Virology 100:1204–1212

11.

Koenig R, Luddecke P, Haeberle AM (1995) Detection of beet necrotic yellow vein virus strains, variants and mixed infections by examining single-strand conformation polymorphisms of immunocapture RT-PCR products. J Gen Virol 76:2051–2055CrossRefPubMed

12.

Henry CM, Barker I, Morris J, Hugo SA (1995) Detection of beet necrotic yellow vein virus using reverse transcription and polymerase chain reaction. J Virol Methods 54:15–28CrossRefPubMed

13.

Wu W, Fan HY, Jiang N, Wang Y, Zhang ZY, Zhang YL, Wang XB, Li DW, Yu JL, Han CG (2014) Infection of beet necrotic yellow vein virus with RNA4-encoded P31 specifically up-regulates pathogenesis-related protein 10 in Nicotiana benthamiana. Virology J 11:118CrossRef

14.

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:e63CrossRefPubMedPubMedCentral

15.

Parida M, Posadas G, Inoue S, Hasebe F, Morita K (2004) Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of west nile virus. J Clin Microbiol 42:257–263CrossRefPubMedPubMedCentral

16.

Hadersdorfer J, Neumuller M, Treutter D, Fischer T (2011) Fast and reliable detection of plum pox virus in woody host plants using the blue LAMP protocol. Ann Appl Biol 159:456–466CrossRef

17.

Tomita N, Mori Y, Kanda H, Notomi T (2008) Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc 3:877–882CrossRefPubMed

18.

Nagamine K, Hase T, Notomi T (2002) Accelerated reaction by loop mediated isothermal amplification using loop primers. Mol Cell Probes 16:223–229CrossRefPubMed

19.

Tsai SM, Chan KW, Hsu WL, Chang TJ, Wong ML, Wang CY (2009) Development of a loop-mediated isothermal amplification for rapid detection of orf virus. J Virol Methods 157:200–204CrossRefPubMed

20.

Parida M, Shukla J, Sharma S, Ranghia-Santhosh S, Ravi V, Mani R, Thomas M, Khare S, Rai A, Kant Ratho R, Pujari S, Mishra B, Lakshmana Rao PV, Vijayaraghavan R (2011) Development and evaluation of reverse transcription loop-mediated isothermal amplification assay for rapid and real-time detection of the swine-origin influenza A H1N1 virus. J Mol Diag 13:100–107CrossRef

21.

Fukuta S, Iida T, Mizukami Y, Ishida A, Ueda J, Kanbe M, Ishimoto Y (2003) Detection of japanese yam mosaic virus by RT-LAMP. Arch Virol 148:1713–1720CrossRefPubMed

22.

Hill J, Beriwal S, Chandra I, Paul VK, Kapil A, Singh T, Wadowsky RM, Singh V, Goyal A, Jahnukainen T, Johnson JR, Tarr PI, Vats A (2008) Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli. J Clin Microbiol 46:2800–2804CrossRefPubMedPubMedCentral

23.

Ren WC, Wang CM, Cai YY (2009) Loop-mediated isothermal amplification for rapid detection of acute viral necrobiotic virus in Scallop chlamys farreri. Acta Virol 53:161–167CrossRefPubMed

24.

Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483CrossRefPubMed

25.

Rowhani A, Stace-Smith R (1979) Purification and characterization of potato leafroll virus. Virology 98:45–54CrossRefPubMed

26.

Almasi MA, Aghapour-ojaghkandi M, Bagheri K, Ghazvini M, Hosseyni-dehabadi SM (2015) Comparison and evaluation of two diagnostic methods for detection of npt II and GUS genes in Nicotiana tabacum. Appl Biochem Biotechnol 175:3599–3616CrossRefPubMed

27.

Almasi MA, Dehabadi SH (2013) Colorimetric immunocapture reverse transcription loop-mediated isothermal amplification assay for rapid detection of the potato virus Y. J Plant Pathol Microb 4:7

28.

Almasi MA, Hosseyni-Dehabadi SM, Aghapour-Ojaghkandi M (2014) Comparison and evaluation of three diagnostic methods for detection of beet curly top virus in sugar beet using different visualizing systems. Appl Biochem Biotechnol 173:1836–1848CrossRefPubMed

29.

Almasi MA, Moradi A, Nasiri J, Karami S, Nasiri M (2012) Assessment of performance ability of three diagnostic methods for detection of potato leafroll virus (PLRV) using different visualizing systems. Appl Biochem Biotechnol 168:770–784CrossRefPubMed

30.

Moradi A, Almasi MA, Jafary H, Mercado-Blanco J (2014) A novel and rapid loop-mediated isothermal amplification assay for the specific detection of Verticillium dahlia. J Appl Microbiol 116:942–954CrossRefPubMed

31.

Dukes JP, King DP, Alexandersen S (2006) Novels reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus. Arch Virol 151:1093–1106CrossRefPubMed

32.

Mori Y, Kitao M, Tomita N, Notomi T (2004) Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Methods 59:145–157CrossRefPubMed

33.

Mori Y, Notomi T (2009) Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother 15:62–69CrossRefPubMed

34.

Nie X (2005) Reverse transcription loop-mediated isothermal amplification of DNA for detection of potato virus Y. Plant Dis 89:605–610CrossRef

Titel: Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus
verfasst von: Mohammad Amin Almasi
Galavizh Almasi
Publikationsdatum: 13.10.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 2/2017
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-3116-0

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