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Archives of Virology

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14.02.2020 | Brief Report

Development of polyclonal-antibody-coated immunomagnetic beads for separation and detection of koi herpesvirus in large-volume samples

verfasst von: Min Zhang, Yingying Li, Hongli Jing, Na Wang, Shaoqiang Wu, Qing Wang, Xiangmei Lin

Erschienen in: Archives of Virology | Ausgabe 4/2020

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Abstract

To separate and concentrate koi herpesvirus (KHV) from large-volume samples, a separation method based on immunomagnetic beads (IMBs) coated with polyclonal antibody directed against KHV was developed. After treatment with IMBs, viral DNA was extracted from samples and used as a template for quantitative PCR (qPCR). The results showed that the concentration of the template DNA extracted from the virus that had been separated using IMBs was 9.65-fold higher than that from virus not treated with IMBs. The detection limit of the IMBs/qPCR method was found to be at least 10 times lower than that of qPCR alone.

Bretzinger AC, Fischer-Scherl T, Oumouna M, Hoffmann R, Truyen U (1999) Mass mortalities in koi carp, Cyprinus carpio, associated with gill and skin disease. Bull Eur Assoc Fish Pathol 19:182–185

Bondad-Reantaso MG, Sunarto A, Subasinghe RP (2007) Managing the koi herpesvirus disease outbreak in Indonesia and the lessons learned. Dev Biol (Basel) 129:21–28

Chen J, Chen KH, Fu BQ, Zhang W, Dai H, Lin LR, Wang LM, He YN (2017) Isolation and identification of senescent renal tubular epithelial cells using immunomagnetic beads based on DcR2. Exp Gerontol 95:116–127CrossRef

Chen MH, Kuo ST, Renault T, Chang PH (2014) The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA. J Virol Methods 196:199–203CrossRef

Eckart V, Yamaguchi T, Franzke K, Bergmann SM, Boudinot P, Quillet E, Kawanobe M, de Haro NA, Fischer U (2019) New cell lines for efficient propagation of koi herpesvirus and infectious salmon anaemia virus. J Fish Dis 42(2):181–187PubMed

Garver KA, Al-Hussinee L, Hawley LM, Schroeder T, Edes S, LePage V, Contador E, Russell S, Lord S, Stevenson RM, Souter B, Wright E, Lumsden JS (2010) Mass mortality associated with koi herpesvirus in wild common carp in Canada. J Wildl Dis 46:1242–1251CrossRef

Gessler F, Hampe K, Schmidt M, Bohnel H (2006) Immunomagnetic beads assay for the detection of botulinum neurotoxin types C and D. Diagn Microbiol Infect Dis 56:225–232CrossRef

Gilad O, Yun S, Zagmutt-Vergara FJ, Leutenegger CM, Bercovier H, Hedrick RP (2004) Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR. Dis Aquat Organ 60:179–187CrossRef

Goldring JP, Brake DA, Cavacini LA, Long CA, Weidanz WP (1989) Cloned T cells provide help for malaria-specific polyclonal antibody responses. Eur J Immunol 19:559–561CrossRef

10.

Haramoto E, Kitajima M, Katayama H, Ohgaki S (2007) Detection of koi herpesvirus DNA in river water in Japan. J Fish Dis 30:59–61CrossRef

11.

Haramoto E, Kitajima M, Katayama H, Ito T, Ohgaki S (2009) Development of virus concentration methods for detection of koi herpesvirus in water. J Fish Dis 32:297–300CrossRef

12.

Hedrick RP, Gilad O, Yun S, Spangenberg JV, Marty GD, Nordhausen RW, Kebus MJ, Bercovier H, Eldar A (2000) A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of common carp. J Aquat Anim Health 12:44–57CrossRef

13.

Kannourakis G, Bol S (1987) Fractionation of normal and beta-thalassemic human hemopoietic progenitor cells by immunomagnetic beads. Exp Hematol 15:1103–1108PubMed

14.

Kim JH, Yoo JG, Ham JS, Oh MH (2018) Direct detection of Escherichia coli, Staphylococcus aureus, and Salmonella spp. in animal-derived foods using a magnetic bead-based immunoassay. Korean J Food Sci Anim Resour 38:727–736PubMedPubMedCentral

15.

Li Y, Wang Q, Bergmann SM, Zeng W, Wang Y, Ren Y, Shi C, Gu D (2019) Preparation of monoclonal antibodies against KHV and establishment of an antigen sandwich ELISA for KHV detection. Microb Pathog 128:36–40CrossRef

16.

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–408CrossRef

17.

Monaghan SJ, Thompson KD, Adams A, Bergmann SM (2015) Sensitivity of seven PCRs for early detection of koi herpesvirus in experimentally infected carp, Cyprinus carpio L., by lethal and non-lethal sampling methods. J Fish Dis 38:303–319CrossRef

18.

Pan Y, Peng X, Yang P, Shi W, Cui S, Zhang D, Lu G, Zhao J, Wang Q (2016) Development of an immunomagnetic beads-based test and its application in influenza surveillance. Clin Chem Lab Med 54:e25–e29PubMed

19.

Prescott MA, Reed AN, Jin L, Pastey MK (2016) Rapid Detection of cyprinid herpesvirus 3 in latently infected koi by recombinase polymerase amplification. J Aquat Anim Health 28:173–180CrossRef

20.

Bergmann SM, Kempter J, Sadowski J, Fichtner D (2006) First detection, confirmation and isolation of koi herpesvirus (KHV) in cultured common carp (Cyprinus carpio L.) in Poland. Bull Eur Assoc Fish Pathol 26:97–104

21.

Sakudo A, Onodera T (2012) Virus capture using anionic polymer-coated magnetic beads (review). Int J Mol Med 30:3–7CrossRef

22.

Sanna G, Lecca V, Foddai A, Tola S (2014) Development of a specific immunomagnetic capture-PCR for rapid detection of viable Mycoplasma agalactiae in sheep milk samples. J Appl Microbiol 117:1585–1591CrossRef

23.

Song F, Zhou Y, Li YS, Meng XM, Meng XY, Liu JQ, Lu SY, Ren HL, Hu P, Liu ZS, Zhang YY, Zhang JH (2014) A rapid immunomagnetic beads-based immunoassay for the detection of beta-casein in bovine milk. Food Chem 158:445–448CrossRef

24.

Waltzek TB, Kelley GO, Stone DM, Way K, Hanson L, Fukuda H, Hirono I, Aoki T, Davison AJ, Hedrick RP (2005) Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae. J Gen Virol 86:1659–1667CrossRef

25.

Wu G, Wang Y, Zhang K, Huang Y, Sun L, Wu C, Li J (2018) Polyclonal antibody and immunomagnetic beads preparation technology for type A trichothecenes. J Hyg Res 47:605–609

26.

Xu XJ, Zhang WH, Kang L, Han FC, Yan Z, Yan XJ (2007) Application of multiple antigen peptide immunomagnetic beads in single-epitope antibody preparation. Chin J Cell Mol Immunol 23:572–574

27.

Yang JJ, Xu XX, Min LF, Lin P, Qian GS (2012) The negative enrichment by immunomagnetic beads for tumor cells from malignant pleural effusions. Chin J Tuberc Respir Dis 35:673–678

28.

Yao L, Wu Q, Wang D, Kou X, Zhang J (2009) Development of monoclonal antibody-coated immunomagnetic beads for separation and detection of norovirus (genogroup II) in faecal extract samples. Lett Appl Microbiol 49:173–178CrossRef

29.

Yatsomboon A, Sermswan RW, Wongratanacheewin S (2018) Development of an immunomagnetic separation-ELISA for the detection of Burkholderia pseudomallei in blood samples. Asian Pac J Allergy Immunol. https://doi.org/10.12932/AP-080518-0307 CrossRefPubMed

Titel: Development of polyclonal-antibody-coated immunomagnetic beads for separation and detection of koi herpesvirus in large-volume samples
verfasst von: Min Zhang
Yingying Li
Hongli Jing
Na Wang
Shaoqiang Wu
Qing Wang
Xiangmei Lin
Publikationsdatum: 14.02.2020
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 4/2020
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04557-y

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Development of polyclonal-antibody-coated immunomagnetic beads for separation and detection of koi herpesvirus in large-volume samples

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