nach oben

Archives of Virology

Erschienen in:

01.08.2011 | Original Article

DNA genome of spontaneously occurring deletion mutants of herpes simplex virus type 1 lacking one copy of the inverted repeat sequences of the L component

verfasst von: Kenichi Umene, Yasuyuki Fukumaki

Erschienen in: Archives of Virology | Ausgabe 8/2011

Einloggen, um Zugang zu erhalten

Abstract

Three non-engineered, spontaneously occurring herpes simplex virus type 1 (HSV-1) mutants (GN52, GN82, and GN91) that have a deletion of approximately 10 kbp (including a part of the UL55 gene, the entire UL56 gene, and one copy of the inverted repeat sequences of the L component (R_L)) and retain the a sequence were isolated. The yields of the mutants at 24 h post-adsorption in cultured cells were comparable to that of an HSV-1 isolate (GN28) without the deletion. Although the three mutants lost one copy of R_L, the L component in replicative intermediates of the mutants inverted. DNA replicative intermediates of the three mutants were flanked by the L component, like those of GN28. The three mutants were generated through recombination involving regions around the authentic cleavage site in the a sequence, suggesting an important role of the a sequence in the diversification of herpesviruses.

Aguilera A, Rothstein R (2007) Molecular genetics of recombination. Springer, BerlinCrossRef

Baines JD, Weller SK (2005) Cleavage and packaging of herpes simplex virus type 1 DNA. In: Catalano CE (ed) Viral genome packaging machines: genetics, structures, and mechanism. Landes Bioscience/Eurekah.com, Texas, pp 135–150

Bataille D, Epstein A (1994) Herpes simplex virus replicative concatemers contain L components in inverted orientation. Virology 203:384–388PubMedCrossRef

Bataille D, Epstein AL (1997) Equimolar generation of the four possible arrangements of adjacent L components in herpes simplex virus type 1 replicative intermediates. J Virol 71:7736–7743PubMed

Bowden R, Sakaoka H, Donnelly P, Ward P (2004) High recombination rate in herpes simplex virus type 1 natural populations suggests significant co-infection. Infect Genet Evol 4:115–123PubMedCrossRef

Bowden RJ, McGeoch DJ (2006) Evolution of herpes simplex viruses. In: Studahl M, Cinque P, Bergström T (eds) Herpes simplex viruses. Taylor & Francis Group, New York

Brown SM, Harland J, Subak-Sharpe JH (1984) Isolation of restriction endonuclease site deletion mutants of herpes simplex virus. J Gen Virol 65:1053–1068PubMedCrossRef

Chou J, Roizman B (1985) Isomerization of herpes simplex virus 1 genome: identification of the cis-acting and recombination sites within the domain of the a sequence. Cell 41:803–811PubMedCrossRef

Cuchet D, Potel C, Thomas J, Epstein AL (2007) HSV-1 amplicon vectors: a promising and versatile tool for gene delivery. Expert Opin Biol Ther 7:975–995PubMedCrossRef

10.

Davison AJ, Marsden HS, Wilkie NM (1981) One functional copy of the long terminal repeat gene specifying the immediate-early polypeptide IE 110 suffices for a productive infection of human foetal lung cells by herpes simplex virus. J Gen Virol 55:179–191PubMedCrossRef

11.

Davison AJ, Wilkie NM (1981) Nucleotide sequences of the joint between the L and S segments of herpes simplex virus types 1 and 2. J Gen Virol 55:315–331PubMedCrossRef

12.

Davison AJ, Wilkie NM (1983) Inversion of the two segments of the herpes simplex virus genome in intertypic recombinants. J Gen Virol 64:1–18PubMedCrossRef

13.

Davison AJ, McGeoch DJ (1986) Evolutionary comparisons of the S segments in the genomes of herpes simplex virus type 1 and varicella-zoster virus. J Gen Virol 67:597–611PubMedCrossRef

14.

Davison AJ, McGeoch DJ (1995) Herpesviridae. In: Gibbs A, Calisher CH, García-Arenal F (eds) Molecular basis of virus evolution. Cambridge University Press, Cambridge, pp 290–309CrossRef

15.

Davison AJ, Eberle R, Hayward GS, McGeoch DJ, Minson AC, Pellett PE, Roizman B, Studdert MJ, Thiry E (2005) Herpesviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy: eighth report of the international committee on taxonomy of viruses. Elsevier Academic Press, London, pp 193–212

16.

Davison AJ, Eberle R, Ehlers B, Hayward GS, McGeoch DJ, Minson AC, Pellett PE, Roizman B, Studdert MJ, Thiry E (2009) The order Herpesvirales. Arch Virol 154:171–177PubMedCrossRef

17.

Harland J, Brown SM (1992) A HSV-1 variant (1720) generates four equimolar isomers despite a 9200-bp deletion from TRL and sequences between 9200 np and 97,000 np in inverted orientation being covalently bound to sequences 94,000–126,372 np. Virus Genes 6:291–299PubMedCrossRef

18.

Inoue R, Moghaddam KA, Ranasinghe M, Saeki Y, Chiocca EA, Wade-Martins R (2004) Infectious delivery of the 132 kb CDKN2A/CDKN2B genomic DNA region results in correctly spliced gene expression and growth suppression in glioma cells. Gene Ther 11:1195–1204PubMedCrossRef

19.

Kwong AD, Frenkel N (1984) Herpes simplex virus amplicon: effect of size on replication of constructed defective genomes containing eucaryotic DNA sequences. J Virol 51:595–603PubMed

20.

Leach DRF (1996) Genetic recombination. Blackwell Science, Oxford

21.

Locker L, Frenkel N (1979) BamHI, KpnI, and SalI restriction enzyme maps of the DNAs of herpes simplex virus strains Justin and F: occurrence of heterogeneities in defined regions of the viral DNA. J Virol 32:429–441PubMed

22.

Longnecker R, Roizman B (1986) Generation of an inverting herpes simplex virus 1 mutant lacking the L-S junction a sequences, an origin of DNA synthesis, and several genes including those specifying glycoprotein E and the α47 gene. J Virol 58:583–591PubMed

23.

MacLean AR, Brown SM (1987) A herpes simplex virus type 1 variant which fails to synthesize immediate early polypeptide V_mwIE63. J Gen Virol 68:1339–1350PubMedCrossRef

24.

MacLean AR, Brown SM (1987) Deletion and duplication variants around the long repeats of herpes simplex virus type 1 strain 17. J Gen Virol 68:3019–3031PubMedCrossRef

25.

MacLean AR, Ul-Fareed M, Robertson L, Harland J, Brown SM (1991) Herpes simplex virus type 1 deletion variants 1714 and 1716 pinpoint neurovirulence-related sequences in Glasgow strain 17⁺ between immediate early gene 1 and the ‘a’ sequence. J Gen Virol 72:631–639PubMedCrossRef

26.

Martin DW, Weber PC (1996) The a sequence is dispensable for isomerization of the herpes simplex virus type 1 genome. J Virol 70:8801–8812PubMed

27.

McGeoch DJ, Dalrymple MA, Davison AJ, Dolan A, Frame MC, McNab D, Perry LJ, Scott JE, Taylor P (1988) The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. J Gen Virol 69:1531–1574PubMedCrossRef

28.

McGeoch DJ, Davison AJ, Dolan A, Gatherer D, Sevilla-Reyers EE (2008) Molecular evolution of the herpesvirales. In: Domingo E, Parrish CR, Holland JJ (eds) Origin and evolution of viruses. Academic Press, London, pp 447–475CrossRef

29.

Meignier B, Longnecker R, Roizman B (1988) In vivo behavior of genetically engineered herpes simplex viruses R7017 and R7020: construction and evaluation in rodents. J Infect Dis 158:602–614PubMedCrossRef

30.

Mocarski ES, Roizman B (1981) Site-specific inversion sequence of the herpes simplex virus genome: domain and structural features. Proc Natl Acad Sci USA 78:7047–7051PubMedCrossRef

31.

Mocarski ES, Roizman B (1982) Structure and role of the herpes simplex virus DNA termini in inversion, circularization and generation of virion DNA. Cell 31:89–97PubMedCrossRef

32.

Mocarski ES, Deiss LP, Frenkel N (1985) Nucleotide sequence and structural features of a novel U_S-a junction present in a defective herpes simplex virus genome. J Virol 55:140–146PubMed

33.

Norberg P, Bergström T, Rekabdar E, Lindh M, Liljeqvist JA (2004) Phylogenetic analysis of clinical herpes simplex virus type 1 isolates identified three genetic groups and recombination viruses. J Virol 78:10755–10764PubMedCrossRef

34.

Norberg P (2010) Divergence and genotyping of human α-herpesviruses: an overview. Infect Genet Evol 10:14–25PubMedCrossRef

35.

Poffenberger KL, Tabares E, Roizman B (1983) Characterization of a viable, noninverting herpes simplex virus 1 genome derived by insertion and deletion of sequences at the junction of components L and S. Proc Natl Acad Sci USA 80:2690–2694PubMedCrossRef

36.

Poffenberger KL, Roizman B (1985) A noninverting genome of a viable herpes simplex virus 1: presence of head-to-tail linkages in packaged genomes and requirements for circularization after infection. J Virol 53:587–595PubMed

37.

Prichard MN, Kaiwar R, Jackman WT, Quenelle DC, Collins DJ, Kern ER, Kemble GM, Spaete RR (2005) Evaluation of AD472, a live attenuated recombinant herpes simplex virus type 2 vaccine in guinea pigs. Vaccine 23:5424–5431PubMedCrossRef

38.

Roizman B (1979) The structure and isomerization of herpes simplex virus genomes. Cell 16:481–494PubMedCrossRef

39.

Roizman B, Desrosiers RC, Fleckenstein B, Lopez C, Minson AC, Studdert MJ (1992) The family Herpesviridae: an update. Arch Virol 123:425–429CrossRef

40.

Samaniego LA, Neiderhiser L, DeLuca NA (1998) Persistence and expression of the herpes simplex virus genome in the absence of immediate-early proteins. J Virol 72:3307–3320PubMed

41.

Sariski RT, Weber PC (1994) Requirement for double-strand breaks but not specific DNA sequences in herpes simplex virus type 1 genome isomerization events. J Virol 68:34–47

42.

Severini A, Scraba DG, Tyrrell DL (1996) Branched structures in the intracellular DNA of herpes simplex virus type 1. J Virol 70:3169–3175PubMed

43.

Skare J, Summers WC (1977) Structure and function of herpesvirus genomes II. EcoRI, XbaI, and HindIII endonuclease cleavage sites on herpes simplex virus type 1 DNA. Virology 76:581–595PubMedCrossRef

44.

Smiley JR, Fong BS, Leung W-C (1981) Construction of a double-joined herpes simplex viral DNA molecule: inverted repeats are required for segment inversion, and direct repeats promote deletions. Virology 113:345–362PubMedCrossRef

45.

Smiley JR, Duncan J, Howes M (1990) Sequence requirements for DNA rearrangements induced by the terminal repeat of herpes simplex virus type 1 KOS DNA. J Virol 64:5036–5050PubMed

46.

Smiley JR, Lavery C, Howes M (1992) The herpes simplex virus type 1 (HSV-1) a sequence serves as a cleavage/packaging signal but does not drive recombinational genome isomerization when it is inserted into the HSV-2 genome. J Virol 66:7505–7510PubMed

47.

Szostak JW, Orr-Weaver TL, Rothstein RJ, Stahl FW (1983) The double-strand-break repair model for recombination. Cell 33:25–35PubMedCrossRef

48.

Szpara ML, Parsons L, Enquist LW (2010) Sequence variability in clinical and laboratory isolates of herpes simplex virus type 1 reveals new mutations. J Virol 84:5303–5313PubMedCrossRef

49.

Taha MY, Clements GB, Brown SM (1989) The herpes simplex virus type 2 (HG52) variant JH2604 has a 1488 bp deletion which eliminates neurovirulence in mice. J Gen Virol 70:3073–3078PubMedCrossRef

50.

Tong L, Stow ND (2010) Analysis of herpes simplex virus type 1 DNA packaging signal mutations in the context of the viral genome. J Virol 84:321–329PubMedCrossRef

51.

Umene K (1986) Conversion of a fraction of the unique sequence to part of the inverted repeats in the S component of the herpes simplex virus type 1 genome. J Gen Virol 67:1035–1048PubMedCrossRef

52.

Umene K (1993) Herpes simplex virus type 1 variant a sequence generated by recombination and breakage of the a sequence in defined regions, including the one involved in recombination. J Virol 67:5685–5691PubMed

53.

Umene K, Yoshida M (1993) Genomic characterization of two predominant genotypes of herpes simplex virus type 1. Arch Virol 131:29–46PubMedCrossRef

54.

Umene K, Nishimoto T (1996) Inhibition of generation of authentic genomic termini of herpes simplex virus type 1 DNA in temperature-sensitive mutant BHK-21 cells with a mutated CCG1/TAF _II 250 gene. J Virol 70:9008–9012PubMed

55.

Umene K (1998) Herpesvirus: genetic variability and recombination. Touka Shobo, Fukuoka

56.

Umene K (1999) Mechanism and application of genetic recombination in herpesviruses. Rev Med Virol 9:171–182PubMedCrossRef

57.

Umene K, Sakaoka H (1999) Evolution of herpes simplex virus type 1 under herpesviral evolutionary processes. Arch Virol 144:637–656PubMedCrossRef

58.

Umene K (2001) Cleavage in and around the DR1 element of the a sequence of herpes simplex virus type 1 relevant to the excision of DNA fragments with length corresponding to one and two units of the a sequence. J Virol 75:5870–5878PubMedCrossRef

59.

Umene K, Oohashi S, Yoshida M, Fukumaki Y (2008) Diversity of the a sequence of herpes simplex virus type 1 developed during evolution. J Gen Virol 89:841–852PubMedCrossRef

60.

Ushijima Y, Luo C, Goshima F, Yamauchi Y, Kimura H, Nishiyama Y (2007) Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus. Microbes Infect 9:142–149PubMedCrossRef

61.

Ushijima Y, Koshizuka T, Goshima F, Kimura H, Nishiyama Y (2008) Herpes simplex virus type 2 UL56 interacts with the ubiquitin ligase Nedd4 and increases its ubiquitination. J Virol 82:5220–5233PubMedCrossRef

62.

Varmuza SL, Smiley JR (1985) Signals for site-specific cleavage of HSV DNA: maturation involves two separate cleavage events at site distal to the recognition sequences. Cell 41:793–802PubMedCrossRef

63.

Wade-Martins R, Smith ER, Tyminski E, Chiocca EA, Saeki Y (2001) An infectious transfer and expression system for genomic DNA loci in human and mouse cells. Nat Biotechnol 19:1067–1070PubMedCrossRef

64.

Wade-Martins R, Saeki Y, Chiocca EA (2003) Infectious delivery of a 135-kb LDLR genomic locus leads to regulated complementation of low-density lipoprotein receptor deficiency in human cells. Mol Ther 7:604–612PubMedCrossRef

65.

Weber PC, Challberg MD, Nelson NJ, Levine M, Glorioso JC (1988) Inversion events in the HSV-1 genome are directly mediated by the viral DNA replication machinery and lack sequence specificity. Cell 54:369–381PubMedCrossRef

66.

Whitton JL, Clements JB (1984) The junctions between the repetitive and the short unique sequences of the herpes simplex virus genome are determined by the polypeptide-coding regions of two spliced immediate-early mRNAs. J Gen Virol 65:451–466PubMedCrossRef

67.

Zhang X, Efstathiou S, Simmons A (1994) Identification of novel herpes simplex virus replicative intermediates by field inversion gel electrophoresis: implications for viral DNA amplification strategies. Virology 202:530–539PubMedCrossRef

Titel: DNA genome of spontaneously occurring deletion mutants of herpes simplex virus type 1 lacking one copy of the inverted repeat sequences of the L component
verfasst von: Kenichi Umene
Yasuyuki Fukumaki
Publikationsdatum: 01.08.2011
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 8/2011
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-0983-2

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Echinokokkose medikamentös behandeln oder operieren?

06.05.2024 DCK 2024 Kongressbericht

Die Therapie von Echinokokkosen sollte immer in spezialisierten Zentren erfolgen. Eine symptomlose Echinokokkose kann – egal ob von Hunde- oder Fuchsbandwurm ausgelöst – konservativ erfolgen. Wenn eine Op. nötig ist, kann es sinnvoll sein, vorher Zysten zu leeren und zu desinfizieren.

Umsetzung der POMGAT-Leitlinie läuft

03.05.2024 DCK 2024 Kongressbericht

Seit November 2023 gibt es evidenzbasierte Empfehlungen zum perioperativen Management bei gastrointestinalen Tumoren (POMGAT) auf S3-Niveau. Vieles wird schon entsprechend der Empfehlungen durchgeführt. Wo es im Alltag noch hapert, zeigt eine Umfrage in einem Klinikverbund.

Proximale Humerusfraktur: Auch 100-Jährige operieren?

01.05.2024 DCK 2024 Kongressbericht

Mit dem demographischen Wandel versorgt auch die Chirurgie immer mehr betagte Menschen. Von Entwicklungen wie Fast-Track können auch ältere Menschen profitieren und bei proximaler Humerusfraktur können selbst manche 100-Jährige noch sicher operiert werden.

Die „Zehn Gebote“ des Endokarditis-Managements

30.04.2024 Endokarditis Leitlinie kompakt

Worauf kommt es beim Management von Personen mit infektiöser Endokarditis an? Eine Kardiologin und ein Kardiologe fassen die zehn wichtigsten Punkte der neuen ESC-Leitlinie zusammen.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 8/2011

Detection of human parechoviruses in children with gastroenteritis in South Korea

Caspase- and p38-MAPK-dependent induction of apoptosis in A549 lung cancer cells by Newcastle disease virus

Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG)

Detection of human rhinoviruses in the lower respiratory tract of lung transplant recipients

The complete genome sequence of a novel T4-like bacteriophage, IME08