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Environmental Health and Preventive Medicine

Erschienen in:

01.05.2010 | Regular Article

Effect of gene polymorphisms and ethanol consumption on micronucleus frequency in human reticulocytes: a preliminary study

verfasst von: Chuancheng Wu, Yuquan Lu, Kanehisa Morimoto

Erschienen in: Environmental Health and Preventive Medicine | Ausgabe 3/2010

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Abstract

Objective

Results from previous studies suggest that alcohol consumption can be genotoxic on peripheral lymphocytes. The aim of our study was to examine the association of alcohol consumption and its genotoxic effect on hematopoietic stem cells in vivo.

Methods

We investigated 156 healthy Japanese males in a cross-sectional study. Lifestyles, including alcohol drinking behavior and cigarette smoking status, were investigated by means of a self-completed questionnaire. Polymorphisms of ADH1B and ALDH2 were identified by PCR–restriction fragment length polymorphism (RFLP) analysis. The presence of micronuclei in transferrin-positive reticulocytes (MN-RET) was detected with a single-laser flow cytometer. Associations between the genetic polymorphisms, lifestyle factors, and MN-RET frequency were statistically analyzed.

Results

We found a significant difference in the mean frequencies of MN-RET between habitual drinkers and non-habitual drinkers (P = 0.043), and between the ALDH2*1/*1 and ALDH2*2/*2 genotype (P = 0.015). The ADH1B*2 and ALDH2*2 haplotype was estimated to have a significantly higher influence on MN-RET frequency than the ADH1B*2 and ALDH2*1 haplotype (P = 0.00035), and the frequency of alcohol consumption played a significant role in MN-RET frequency on the background of the ADH1B*2 and ALDH2*1 haplotype (P = 0.012).

Conclusion

The results of our study suggest a possible association between the ADH1B and ALDH2 polymorphism and the genotoxic effects of alcohol drinking on hematopoietic stem cells.

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Titel: Effect of gene polymorphisms and ethanol consumption on micronucleus frequency in human reticulocytes: a preliminary study
verfasst von: Chuancheng Wu
Yuquan Lu
Kanehisa Morimoto
Publikationsdatum: 01.05.2010
Verlag: Springer Japan
Erschienen in: Environmental Health and Preventive Medicine / Ausgabe 3/2010
Print ISSN: 1342-078X
Elektronische ISSN: 1347-4715
DOI: https://doi.org/10.1007/s12199-009-0126-5

Springer Medizin

Abstract

Objective

Methods

Results

Conclusion

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