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01.12.2015 | Research article | Ausgabe 1/2015 Open Access

BMC Oral Health 1/2015

Effect of method of caries induction on aged resin-dentin bond of primary teeth

Zeitschrift:
BMC Oral Health > Ausgabe 1/2015
Autoren:
Tathiane Larissa Lenzi, Ana Flávia Bissoto Calvo, Tamara Kerber Tedesco, Hérica Adad Ricci, Josimeri Hebling, Daniela Prócida Raggio
Wichtige Hinweise

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

TLL: idea, performed the experiments, wrote the manuscript. AFBC: contribution substantially to discussion, proofread the manuscript. TKT: contribution substantially to discussion, proofread the manuscript. HAR: contributions to the interpretation of the results, proofread the manuscript. JH: contributions to the interpretation of the results, proofread the manuscript. DPR: experimental design, consulted on and performed statistical evaluation, proofread the manuscript. All authors have read and approved the final version of the manuscript.

Authors’ information

TLL: PhD Student, Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo AFBC: PhD Student, Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Brazil; TKT: PhD Student, Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Brazil; HAR: PhD Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, São Paulo State University – UNESP, Araraquara, SP, Brazil and Physics Institute of São Carlos, University of São Paulo – USP, São Carlos, SP, Brazil; JH: Professor, Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, São Paulo State University – UNESP, Araraquara, SP, Brazil, DPR: Associate Professor, Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Brazil.

Abstract

Background

To investigate the influence of chemical and microbiological methods of caries induction on bond degradation of adhesive systems to primary dentin.

Methods

Flat dentin surfaces from 36 primary molars were assigned to three groups (n = 12) according to method to induce caries-affected dentin: (1) control (sound dentin); (2) pH-cycling; and (3) microbiological caries induction model. Teeth were submitted to caries induction for 14 days for both methods, and the sound dentin was stored in distilled water during the same period. Specimens from each experimental group were then randomly reassigned to two subgroups (n = 6) according to the adhesive system tested: two-step etch-and-rinse adhesive (Adper Single Bond 2 - SB) or two-step self-etch system (Clearfil SE Bond - CSEB). Composite buildups were constructed and sectioned to obtain bonded sticks to be subjected to microtensile (μTBS) testing immediately or after 12 months of water aging. The μTBS means were analyzed by three-way repeated measures ANOVA and Tukey’s tests (α = 0.05).

Results

The μTBS values obtained to artificially-created caries-affected dentin were lower compared with sound dentin, but were not affected by method of caries induction. Water storage for 12 months reduced bond strengths, except to CSEB bonded to sound dentin.

Conclusion

Chemical and microbiological methods affect similarly the stability of resin-dentin bonds in primary teeth.
Literatur
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