Skip to main content
Erschienen in: Clinical Oral Investigations 3/2010

01.06.2010 | Original Article

Effect of mouthrinses on Aggregatibacter actinomycetemcomitans biofilms in a hydrodynamic model

verfasst von: Isabelle Sliepen, Mark Van Essche, Marc Quirynen, Wim Teughels

Erschienen in: Clinical Oral Investigations | Ausgabe 3/2010

Einloggen, um Zugang zu erhalten

Abstract

The aim of the study was to evaluate the effects of Listerine®, Meridol®, and Perioaid® on the viability and total number of bacteria in established biofilms using an in vitro model under hydrodynamic conditions. Biofilms of Aggregatibacter actinomycetemcomitans were placed in a modified Robbins device and rinsed twice daily during 4 days. Bacteria were quantified by culture and quantitative polymerase chain reaction. Visualization of the samples was performed by scanning electron and confocal laser scanning microscopy, combined with a fluorescent vital staining. All three mouthrinses caused a significant reduction in the number of cultivable A. actinomycetemcomitans in a biofilm. Perioaid® was significantly the most powerful in killing the biofilm-protected bacteria and also in counteracting the development of thick dense microbial communities. The total amount of bacteria was not significantly affected by Listerine® and Meridol®.
Literatur
1.
Zurück zum Zitat Botelho MA, Bezerra Filho JG, Correa LL, Fonseca SG, Montenegro D, Gapski R, Brito GA, Heukelbach J (2007) Effect of a novel essential oil mouthrinse without alcohol on gingivitis: a double-blinded randomized controlled trial. J Appl Oral Sci 15:175–180CrossRefPubMed Botelho MA, Bezerra Filho JG, Correa LL, Fonseca SG, Montenegro D, Gapski R, Brito GA, Heukelbach J (2007) Effect of a novel essential oil mouthrinse without alcohol on gingivitis: a double-blinded randomized controlled trial. J Appl Oral Sci 15:175–180CrossRefPubMed
2.
Zurück zum Zitat Quirynen M, Papaioannou W, van Steenbergen TJ, Dierickx K, Cassiman JJ, van Steenberghe D (2001) Adhesion of Porphyromonas gingivalis strains to cultured epithelial cells from patients with a history of chronic adult periodontitis or from patients less susceptible to periodontitis. J Periodontol 72:626–633CrossRefPubMed Quirynen M, Papaioannou W, van Steenbergen TJ, Dierickx K, Cassiman JJ, van Steenberghe D (2001) Adhesion of Porphyromonas gingivalis strains to cultured epithelial cells from patients with a history of chronic adult periodontitis or from patients less susceptible to periodontitis. J Periodontol 72:626–633CrossRefPubMed
3.
Zurück zum Zitat Silverman S, Wilder R (2006) Antimicrobial mouthrinse as part of a comprehensive oral care regimen. Safety and compliance factors. J Am Dent Assoc 137:22S–26SPubMed Silverman S, Wilder R (2006) Antimicrobial mouthrinse as part of a comprehensive oral care regimen. Safety and compliance factors. J Am Dent Assoc 137:22S–26SPubMed
4.
Zurück zum Zitat Mandell RL, Socransky SS (1981) A selective medium for Actinobacillus actinomycetemcomitans and the incidence of the organism in juvenile periodontitis. J Periodontol 52:593–598PubMed Mandell RL, Socransky SS (1981) A selective medium for Actinobacillus actinomycetemcomitans and the incidence of the organism in juvenile periodontitis. J Periodontol 52:593–598PubMed
5.
Zurück zum Zitat Slots J (1999) Update on Actinobacillus Actinomycetemcomitans and Porphyromonas gingivalis in human periodontal disease. J Int Acad Periodontol 1:121–126PubMed Slots J (1999) Update on Actinobacillus Actinomycetemcomitans and Porphyromonas gingivalis in human periodontal disease. J Int Acad Periodontol 1:121–126PubMed
6.
Zurück zum Zitat Overholt BF (1966) Actinobacillus actinomycetemcomitans endocarditis. Arch Intern Med 117:99–102CrossRefPubMed Overholt BF (1966) Actinobacillus actinomycetemcomitans endocarditis. Arch Intern Med 117:99–102CrossRefPubMed
7.
Zurück zum Zitat Muhle I, Rau J, Ruskin J (1979) Vertebral osteomyelitis due to Actinobacillus actinomycetemcomitans. JAMA 241:1824–1825CrossRefPubMed Muhle I, Rau J, Ruskin J (1979) Vertebral osteomyelitis due to Actinobacillus actinomycetemcomitans. JAMA 241:1824–1825CrossRefPubMed
8.
Zurück zum Zitat Martin BF, Derby BM, Budzilovich GN, Ransohoff J (1967) Brain abscess due to Actinobacillus actinomycetemcomitans. Neurology 17:833–837PubMed Martin BF, Derby BM, Budzilovich GN, Ransohoff J (1967) Brain abscess due to Actinobacillus actinomycetemcomitans. Neurology 17:833–837PubMed
9.
Zurück zum Zitat Kinniment SL, Wimpenny JW, Adams D, Marsh PD (1996) The effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system. J Appl Bacteriol 81:120–125PubMed Kinniment SL, Wimpenny JW, Adams D, Marsh PD (1996) The effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system. J Appl Bacteriol 81:120–125PubMed
10.
Zurück zum Zitat Haase EM, Bonstein T, Palmer RJ Jr, Scannapieco FA (2006) Environmental influences on Actinobacillus actinomycetemcomitans biofilm formation. Arch Oral Biol 51:299–314CrossRefPubMed Haase EM, Bonstein T, Palmer RJ Jr, Scannapieco FA (2006) Environmental influences on Actinobacillus actinomycetemcomitans biofilm formation. Arch Oral Biol 51:299–314CrossRefPubMed
11.
Zurück zum Zitat Ouhayoun JP (2003) Penetrating the plaque biofilm: impact of essential oil mouthwash. J Clin Periodontol 30(Suppl 5):10–12CrossRefPubMed Ouhayoun JP (2003) Penetrating the plaque biofilm: impact of essential oil mouthwash. J Clin Periodontol 30(Suppl 5):10–12CrossRefPubMed
12.
Zurück zum Zitat Madlena M, Dombi C, Gintner Z, Banoczy J (2004) Effect of amine fluoride/stannous fluoride toothpaste and mouthrinse on dental plaque accumulation and gingival health. Oral Dis 10:294–297CrossRefPubMed Madlena M, Dombi C, Gintner Z, Banoczy J (2004) Effect of amine fluoride/stannous fluoride toothpaste and mouthrinse on dental plaque accumulation and gingival health. Oral Dis 10:294–297CrossRefPubMed
13.
Zurück zum Zitat Keijser JA, Verkade H, Timmerman MF, Van der Weijden FA (2003) Comparison of 2 commercially available chlorhexidine mouthrinses. J Periodontol 74:214–218CrossRefPubMed Keijser JA, Verkade H, Timmerman MF, Van der Weijden FA (2003) Comparison of 2 commercially available chlorhexidine mouthrinses. J Periodontol 74:214–218CrossRefPubMed
14.
Zurück zum Zitat Wolff LF (1985) Chemotherapeutic agents in the prevention and treatment of periodontal disease. Northwest Dent 64:15–24PubMed Wolff LF (1985) Chemotherapeutic agents in the prevention and treatment of periodontal disease. Northwest Dent 64:15–24PubMed
15.
Zurück zum Zitat Fine DH (1988) Mouthrinses as adjuncts for plaque and gingivitis management. A status report for the American Journal of Dentistry. Am J Dent 1:259–263PubMed Fine DH (1988) Mouthrinses as adjuncts for plaque and gingivitis management. A status report for the American Journal of Dentistry. Am J Dent 1:259–263PubMed
16.
Zurück zum Zitat Arweiler NB, Boehnke N, Sculean A, Hellwig E, Auschill TM (2006) Differences in efficacy of two commercial 0.2% chlorhexidine mouthrinse solutions: a 4-day plaque re-growth study. J Clin Periodontol 33:334–339CrossRefPubMed Arweiler NB, Boehnke N, Sculean A, Hellwig E, Auschill TM (2006) Differences in efficacy of two commercial 0.2% chlorhexidine mouthrinse solutions: a 4-day plaque re-growth study. J Clin Periodontol 33:334–339CrossRefPubMed
17.
Zurück zum Zitat Busscher HJ, van der Mei HC (2006) Microbial adhesion in flow displacement systems. Clin Microbiol Rev 19:127–141CrossRefPubMed Busscher HJ, van der Mei HC (2006) Microbial adhesion in flow displacement systems. Clin Microbiol Rev 19:127–141CrossRefPubMed
18.
Zurück zum Zitat Boutaga K, van Winkelhoff AJ, Vandenbroucke-Grauls CM, Savelkoul PH (2005) Periodontal pathogens: a quantitative comparison of anaerobic culture and real-time PCR. FEMS Immunol Med Microbiol 45:191–199CrossRefPubMed Boutaga K, van Winkelhoff AJ, Vandenbroucke-Grauls CM, Savelkoul PH (2005) Periodontal pathogens: a quantitative comparison of anaerobic culture and real-time PCR. FEMS Immunol Med Microbiol 45:191–199CrossRefPubMed
19.
Zurück zum Zitat Bretz F, Genz A, Hothorn LA (2001) On the numerical availability of multiple comparison procedures. Biom J 43:645–656CrossRef Bretz F, Genz A, Hothorn LA (2001) On the numerical availability of multiple comparison procedures. Biom J 43:645–656CrossRef
20.
Zurück zum Zitat Bergmans L, Moisiadis P, Van Meerbeek B, Quirynen M, Lambrechts P (2005) Microscopic observation of bacteria: review highlighting the use of environmental SEM. Int Endod J 38:775–788CrossRefPubMed Bergmans L, Moisiadis P, Van Meerbeek B, Quirynen M, Lambrechts P (2005) Microscopic observation of bacteria: review highlighting the use of environmental SEM. Int Endod J 38:775–788CrossRefPubMed
21.
Zurück zum Zitat Cummins D, Creeth JE (1992) Delivery of antiplaque agents from dentifrices, gels, and mouthwashes. J Dent Res 71:1439–1449PubMed Cummins D, Creeth JE (1992) Delivery of antiplaque agents from dentifrices, gels, and mouthwashes. J Dent Res 71:1439–1449PubMed
22.
Zurück zum Zitat McClanahan SF, Bartizek RD (2002) Effects of triclosan/copolymer dentifrice on dental plaque and gingivitis in a 3-month randomized controlled clinical trial: influence of baseline gingivitis on observed efficacy. J Clin Dent 13:167–178PubMed McClanahan SF, Bartizek RD (2002) Effects of triclosan/copolymer dentifrice on dental plaque and gingivitis in a 3-month randomized controlled clinical trial: influence of baseline gingivitis on observed efficacy. J Clin Dent 13:167–178PubMed
23.
Zurück zum Zitat Riep BG, Bernimoulin JP, Barnett ML (1999) Comparative antiplaque effectiveness of an essential oil and an amine fluoride/stannous fluoride mouthrinse. J Clin Periodontol 26:164–168CrossRefPubMed Riep BG, Bernimoulin JP, Barnett ML (1999) Comparative antiplaque effectiveness of an essential oil and an amine fluoride/stannous fluoride mouthrinse. J Clin Periodontol 26:164–168CrossRefPubMed
24.
Zurück zum Zitat Arweiler NB, Netuschil L, Reich E (2001) Alcohol-free mouthrinse solutions to reduce supragingival plaque regrowth and vitality. A controlled clinical study. J Clin Periodontol 28:168–174CrossRefPubMed Arweiler NB, Netuschil L, Reich E (2001) Alcohol-free mouthrinse solutions to reduce supragingival plaque regrowth and vitality. A controlled clinical study. J Clin Periodontol 28:168–174CrossRefPubMed
25.
Zurück zum Zitat Charles CH, Mostler KM, Bartels LL, Mankodi SM (2004) Comparative antiplaque and antigingivitis effectiveness of a chlorhexidine and an essential oil mouthrinse: 6-month clinical trial. J Clin Periodontol 3:878–884CrossRef Charles CH, Mostler KM, Bartels LL, Mankodi SM (2004) Comparative antiplaque and antigingivitis effectiveness of a chlorhexidine and an essential oil mouthrinse: 6-month clinical trial. J Clin Periodontol 3:878–884CrossRef
26.
Zurück zum Zitat Overholser CD, Meiller TF, DePaola LG, Minah GE, Niehaus C (1990) Comparative effects of 2 chemotherapeutic mouthrinses on the development of supragingival dental plaque and gingivitis. J Clin Periodontol 17:575–579CrossRefPubMed Overholser CD, Meiller TF, DePaola LG, Minah GE, Niehaus C (1990) Comparative effects of 2 chemotherapeutic mouthrinses on the development of supragingival dental plaque and gingivitis. J Clin Periodontol 17:575–579CrossRefPubMed
27.
Zurück zum Zitat Larsen T (2002) Susceptibility of Porphyromonas gingivalis in biofilms to amoxicillin, doxycycline and metronidazole. Oral Microbiol Immunol 17:267–271CrossRefPubMed Larsen T (2002) Susceptibility of Porphyromonas gingivalis in biofilms to amoxicillin, doxycycline and metronidazole. Oral Microbiol Immunol 17:267–271CrossRefPubMed
28.
Zurück zum Zitat Costerton JW, Stewart PS, Greenberg EP (1999) Bacterial biofilms: a common cause of persistent infections. Science 284:1318–1322CrossRefPubMed Costerton JW, Stewart PS, Greenberg EP (1999) Bacterial biofilms: a common cause of persistent infections. Science 284:1318–1322CrossRefPubMed
29.
Zurück zum Zitat Shapiro S, Giertsen E, Guggenheim B (2002) An in vitro oral biofilm model for comparing the efficacy of antimicrobial mouthrinses. Caries Res 36:93–100CrossRefPubMed Shapiro S, Giertsen E, Guggenheim B (2002) An in vitro oral biofilm model for comparing the efficacy of antimicrobial mouthrinses. Caries Res 36:93–100CrossRefPubMed
30.
Zurück zum Zitat Fine DH, Furgang D, Barnett ML (2001) Comparative antimicrobial activities of antiseptic mouthrinses against isogenic planktonic and biofilm forms of Actinobacillus actinomycetemcomitans. J Clin Periodontol 28:697–700CrossRefPubMed Fine DH, Furgang D, Barnett ML (2001) Comparative antimicrobial activities of antiseptic mouthrinses against isogenic planktonic and biofilm forms of Actinobacillus actinomycetemcomitans. J Clin Periodontol 28:697–700CrossRefPubMed
31.
Zurück zum Zitat Larsen T, Fiehn NE (1995) Development of a flow method for susceptibility testing of oral biofilms in vitro. APMIS 103:339–344CrossRefPubMed Larsen T, Fiehn NE (1995) Development of a flow method for susceptibility testing of oral biofilms in vitro. APMIS 103:339–344CrossRefPubMed
32.
Zurück zum Zitat Noiri Y, Okami Y, Narimatsu M, Takahashi Y, Kawahara T, Ebisu S (2003) Effects of chlorhexidine, minocycline, and metronidazole on Porphyromonas gingivalis strain 381 in biofilms. J Periodontol 74:1647–1651CrossRefPubMed Noiri Y, Okami Y, Narimatsu M, Takahashi Y, Kawahara T, Ebisu S (2003) Effects of chlorhexidine, minocycline, and metronidazole on Porphyromonas gingivalis strain 381 in biofilms. J Periodontol 74:1647–1651CrossRefPubMed
33.
Zurück zum Zitat Busscher HJ, Free RH, Van Weissenbruch R, Albers FW, van der Mei HC (2000) Preliminary observations on influence of dairy products on biofilm removal from silicone rubber voice prostheses in vitro. J Dairy Sci 83:641–647CrossRefPubMed Busscher HJ, Free RH, Van Weissenbruch R, Albers FW, van der Mei HC (2000) Preliminary observations on influence of dairy products on biofilm removal from silicone rubber voice prostheses in vitro. J Dairy Sci 83:641–647CrossRefPubMed
34.
Zurück zum Zitat Sliepen I, Van Essche M, Pauwels M, Van Eldere J, Hofkens J, Quirynen M, Teughels W (2008) Colonization of hard and soft surfaces by Aggregatibacter actinomycetemcomitans under hydrodynamic conditions. Oral Microbiol Immunol 23:498–504CrossRefPubMed Sliepen I, Van Essche M, Pauwels M, Van Eldere J, Hofkens J, Quirynen M, Teughels W (2008) Colonization of hard and soft surfaces by Aggregatibacter actinomycetemcomitans under hydrodynamic conditions. Oral Microbiol Immunol 23:498–504CrossRefPubMed
35.
Zurück zum Zitat Sliepen I, Hofkens J, Van Essche M, Quirynen M, Teughels W (2008) Aggregatibacter actinomycetemcomitans adhesion inhibited in a flow cell. Oral Microbiol Immunol 23:520–524CrossRefPubMed Sliepen I, Hofkens J, Van Essche M, Quirynen M, Teughels W (2008) Aggregatibacter actinomycetemcomitans adhesion inhibited in a flow cell. Oral Microbiol Immunol 23:520–524CrossRefPubMed
36.
Zurück zum Zitat Bercy P, Lasserre J (2007) Susceptibility to various oral antiseptics of Porphyromonas gingivalis W83 within a biofilm. Adv Ther 24:1181–1191CrossRefPubMed Bercy P, Lasserre J (2007) Susceptibility to various oral antiseptics of Porphyromonas gingivalis W83 within a biofilm. Adv Ther 24:1181–1191CrossRefPubMed
37.
Zurück zum Zitat Paraskevas S (2005) Randomized controlled clinical trials on agents used for chemical plaque control. Int J Dent Hyg 3:162–178CrossRefPubMed Paraskevas S (2005) Randomized controlled clinical trials on agents used for chemical plaque control. Int J Dent Hyg 3:162–178CrossRefPubMed
38.
Zurück zum Zitat Van Strydonck DA, Timmerman MF, van der Weijden GA (2005) Plaque inhibition of two commercially available chlorhexidine mouthrinses. J Clin Periodontol 32:305–309CrossRefPubMed Van Strydonck DA, Timmerman MF, van der Weijden GA (2005) Plaque inhibition of two commercially available chlorhexidine mouthrinses. J Clin Periodontol 32:305–309CrossRefPubMed
39.
Zurück zum Zitat Brecx M, Netuschil L, Reichert B, Schreil G (1990) Efficacy of Listerine, Meridol and chlorhexidine mouthrinses on plaque, gingivitis and plaque bacteria vitality. J Clin Periodontol 17:292–297CrossRefPubMed Brecx M, Netuschil L, Reichert B, Schreil G (1990) Efficacy of Listerine, Meridol and chlorhexidine mouthrinses on plaque, gingivitis and plaque bacteria vitality. J Clin Periodontol 17:292–297CrossRefPubMed
40.
Zurück zum Zitat Netuschil L, Weiger R, Preisler R, Brecx M (1995) Plaque bacteria counts and vitality during chlorhexidine, Meridol and Listerine mouthrinses. Eur J Oral Sci 103:355–361CrossRefPubMed Netuschil L, Weiger R, Preisler R, Brecx M (1995) Plaque bacteria counts and vitality during chlorhexidine, Meridol and Listerine mouthrinses. Eur J Oral Sci 103:355–361CrossRefPubMed
41.
Zurück zum Zitat Brecx M, Brownstone E, MacDonald L, Gelskey S, Cheang M (1992) Efficacy of Listerine, Meridol and chlorhexidine mouthrinses as supplements to regular tooth cleaning measures. J Clin Periodontol 19:202–207CrossRefPubMed Brecx M, Brownstone E, MacDonald L, Gelskey S, Cheang M (1992) Efficacy of Listerine, Meridol and chlorhexidine mouthrinses as supplements to regular tooth cleaning measures. J Clin Periodontol 19:202–207CrossRefPubMed
42.
Zurück zum Zitat Gunsolley JC (2006) A meta-analysis of six-month studies of antiplaque and antigingivitis agents. J Am Dent Assoc 137:1649–1657PubMed Gunsolley JC (2006) A meta-analysis of six-month studies of antiplaque and antigingivitis agents. J Am Dent Assoc 137:1649–1657PubMed
43.
Zurück zum Zitat Stoeken JE, Paraskevas S, van der Weijden GA (2007) The long-term effect of a mouthrinse containing essential oils on dental plaque and gingivitis: a systematic review. J Periodontol 78:1218–1228CrossRefPubMed Stoeken JE, Paraskevas S, van der Weijden GA (2007) The long-term effect of a mouthrinse containing essential oils on dental plaque and gingivitis: a systematic review. J Periodontol 78:1218–1228CrossRefPubMed
44.
Zurück zum Zitat Fine DH, Furgang D, Schreiner HC, Goncharoff P, Charlesworth J, Ghazwan G, Fitzgerald-Bocarsly P, Figurski DH (1999) Phenotypic variation in Actinobacillus actinomycetemcomitans during laboratory growth: implications for virulence. Microbiol 145:1335–1347CrossRef Fine DH, Furgang D, Schreiner HC, Goncharoff P, Charlesworth J, Ghazwan G, Fitzgerald-Bocarsly P, Figurski DH (1999) Phenotypic variation in Actinobacillus actinomycetemcomitans during laboratory growth: implications for virulence. Microbiol 145:1335–1347CrossRef
45.
Zurück zum Zitat Fine DH, Kaplan JB, Kachlany SC, Schreiner HC (2006) How we got attached to Actinobacillus actinomycetemcomitans: a model for infectious diseases. Periodontol 2000(42):114–157CrossRef Fine DH, Kaplan JB, Kachlany SC, Schreiner HC (2006) How we got attached to Actinobacillus actinomycetemcomitans: a model for infectious diseases. Periodontol 2000(42):114–157CrossRef
46.
Zurück zum Zitat Zambon JJ, Slots J, Genco RJ (1983) Serology of oral Actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease. Infect Immun 41:19–27PubMed Zambon JJ, Slots J, Genco RJ (1983) Serology of oral Actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease. Infect Immun 41:19–27PubMed
47.
Zurück zum Zitat Okte E, Sultan N, Dogan B, Asikainen S (1999) Bacterial adhesion of Actinobacillus actinomycetemcomitans serotypes to titanium implants: SEM evaluation. A preliminary report. J Periodontol 70:1376–1382CrossRefPubMed Okte E, Sultan N, Dogan B, Asikainen S (1999) Bacterial adhesion of Actinobacillus actinomycetemcomitans serotypes to titanium implants: SEM evaluation. A preliminary report. J Periodontol 70:1376–1382CrossRefPubMed
48.
Zurück zum Zitat Yang HW, Asikainen S, Dogan B, Suda R, Lai CH (2004) Relationship of Actinobacillus actinomycetemcomitans serotype b to aggressive periodontitis: frequency in pure cultured isolates. J Periodontol 75:592–599CrossRefPubMed Yang HW, Asikainen S, Dogan B, Suda R, Lai CH (2004) Relationship of Actinobacillus actinomycetemcomitans serotype b to aggressive periodontitis: frequency in pure cultured isolates. J Periodontol 75:592–599CrossRefPubMed
49.
Zurück zum Zitat Imbert-Bernard C, Valentin A, Reynes J, Mallie M, Bastide JM (1994) Relationship between fluconazole sensitivity of Candida albicans isolates from HIV positive patients and serotype, adherence and CD4+ lymphocyte count. Eur J Clin Microbiol Infect Dis 13:711–716CrossRefPubMed Imbert-Bernard C, Valentin A, Reynes J, Mallie M, Bastide JM (1994) Relationship between fluconazole sensitivity of Candida albicans isolates from HIV positive patients and serotype, adherence and CD4+ lymphocyte count. Eur J Clin Microbiol Infect Dis 13:711–716CrossRefPubMed
50.
Zurück zum Zitat Abbott JD, Graves JF (1972) Serotype and sulphonamide sensitivity of meningococci isolated from 1966 to 1971. J Clin Pathol 25:528–530CrossRefPubMed Abbott JD, Graves JF (1972) Serotype and sulphonamide sensitivity of meningococci isolated from 1966 to 1971. J Clin Pathol 25:528–530CrossRefPubMed
51.
Zurück zum Zitat van der Mei HC, White DJ, Atema-Smit J, Belt-Gritter E, Busscher HJ (2006) A method to study sustained antimicrobial activity of rinse and dentifrice components on biofilm viability in vivo. J Clin Periodontol 33:14–20CrossRefPubMed van der Mei HC, White DJ, Atema-Smit J, Belt-Gritter E, Busscher HJ (2006) A method to study sustained antimicrobial activity of rinse and dentifrice components on biofilm viability in vivo. J Clin Periodontol 33:14–20CrossRefPubMed
52.
Zurück zum Zitat Inoue T, Shingaki R, Sogawa N, Sogawa CA, Asaumi J, Kokeguchi S (2003) Biofilm formation by a fimbriae-deficient mutant of Actinobacillus actinomycetemcomitans. Microbiol Immunol 47:877–881PubMed Inoue T, Shingaki R, Sogawa N, Sogawa CA, Asaumi J, Kokeguchi S (2003) Biofilm formation by a fimbriae-deficient mutant of Actinobacillus actinomycetemcomitans. Microbiol Immunol 47:877–881PubMed
53.
Zurück zum Zitat Izano EA, Wang H, Ragunath C, Ramasubbu N, Kaplan JB (2007) Detachment and killing of Aggregatibacter actinomycetemcomitans biofilms by dispersin B and SDS. J Dent Res 86:618–622CrossRefPubMed Izano EA, Wang H, Ragunath C, Ramasubbu N, Kaplan JB (2007) Detachment and killing of Aggregatibacter actinomycetemcomitans biofilms by dispersin B and SDS. J Dent Res 86:618–622CrossRefPubMed
54.
Zurück zum Zitat Whitchurch CB, Tolker-Nielsen T, Ragas PC, Mattick JS (2002) Extracellular DNA required for bacterial biofilm formation. Science 295:1487CrossRefPubMed Whitchurch CB, Tolker-Nielsen T, Ragas PC, Mattick JS (2002) Extracellular DNA required for bacterial biofilm formation. Science 295:1487CrossRefPubMed
55.
Zurück zum Zitat Fine DH, Markowitz K, Furgang D, Goldsmith D, Ricci-Nittel D, Charles CH, Peng P, Lynch MC (2007) Effect of rinsing with an essential oil-containing mouthrinse on subgingival periodontopathogens. J Periodontol 78:1935–1942CrossRefPubMed Fine DH, Markowitz K, Furgang D, Goldsmith D, Ricci-Nittel D, Charles CH, Peng P, Lynch MC (2007) Effect of rinsing with an essential oil-containing mouthrinse on subgingival periodontopathogens. J Periodontol 78:1935–1942CrossRefPubMed
Metadaten
Titel
Effect of mouthrinses on Aggregatibacter actinomycetemcomitans biofilms in a hydrodynamic model
verfasst von
Isabelle Sliepen
Mark Van Essche
Marc Quirynen
Wim Teughels
Publikationsdatum
01.06.2010
Verlag
Springer-Verlag
Erschienen in
Clinical Oral Investigations / Ausgabe 3/2010
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-009-0286-0

Weitere Artikel der Ausgabe 3/2010

Clinical Oral Investigations 3/2010 Zur Ausgabe

Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Zahnmedizin und bleiben Sie gut informiert – ganz bequem per eMail.