Erschienen in:
01.06.2010 | Original Article
Effect of mouthrinses on Aggregatibacter actinomycetemcomitans biofilms in a hydrodynamic model
verfasst von:
Isabelle Sliepen, Mark Van Essche, Marc Quirynen, Wim Teughels
Erschienen in:
Clinical Oral Investigations
|
Ausgabe 3/2010
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Abstract
The aim of the study was to evaluate the effects of Listerine®, Meridol®, and Perioaid® on the viability and total number of bacteria in established biofilms using an in vitro model under hydrodynamic conditions. Biofilms of Aggregatibacter actinomycetemcomitans were placed in a modified Robbins device and rinsed twice daily during 4 days. Bacteria were quantified by culture and quantitative polymerase chain reaction. Visualization of the samples was performed by scanning electron and confocal laser scanning microscopy, combined with a fluorescent vital staining. All three mouthrinses caused a significant reduction in the number of cultivable A. actinomycetemcomitans in a biofilm. Perioaid® was significantly the most powerful in killing the biofilm-protected bacteria and also in counteracting the development of thick dense microbial communities. The total amount of bacteria was not significantly affected by Listerine® and Meridol®.