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International Orthopaedics
Erschienen in: International Orthopaedics 4/2012

01.04.2012 | Original Paper

Effect of rat brain tissue extracts on osteoblast proliferation and differentiation

verfasst von: Gang-Yong Huang, Xin Ma, Xin-Lei Xia, Jian-Yuan Jiang, Wei-Fang Jin, Jian-Jun Gao, Huang-Yuan Huang

Erschienen in: International Orthopaedics | Ausgabe 4/2012

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Abstract

Purpose

The reason for enhanced fracture healing in traumatic brain injury patients is not clearly understood. It is possible that factors inherent in the brain passing through the blood–brain barrier to the peripheral circulation, or a disruption of central nervous system (CNS) control of the sympathetic nervous system (SNS), stimulates the process of fracture healing.

Methods

In this study, we assessed proliferation [using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay] and differentiation [using alkaline phosphatase (ALP)] in rat osteoblasts incubated with gray matter or other tissue extracts with and without the addition of an α- or β-adrenergic receptor blocker (phentolamine or propranolol).

Results

Gray matter extract from normal brain caused a dose-dependent increase in osteoblast proliferation and differentiation. Serum from normal rats enhanced differentiation but not proliferation. Alpha-receptor blockade had no effect on proliferation or differentiation. Beta-receptor blockade caused a partial, but statistically significant, decrease in gray matter stimulation of osteoblast differentiation.

Conclusion

The results of this study indicate that gray matter extract from normal brain increases osteoblast proliferation and differentiation and that β receptors may be involved in differentiation under these conditions.
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Metadaten
Titel
Effect of rat brain tissue extracts on osteoblast proliferation and differentiation
verfasst von
Gang-Yong Huang
Xin Ma
Xin-Lei Xia
Jian-Yuan Jiang
Wei-Fang Jin
Jian-Jun Gao
Huang-Yuan Huang
Publikationsdatum
01.04.2012
Verlag
Springer-Verlag
Erschienen in
International Orthopaedics / Ausgabe 4/2012
Print ISSN: 0341-2695
Elektronische ISSN: 1432-5195
DOI
https://doi.org/10.1007/s00264-011-1423-3

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