Background
Methods
Animals and experimental grouping
Drug preparation
Voiding stain on paper (VSOP) analysis
Urodynamic test
In vitro experimental protocols
ATP release measurements
RT-PCR
cDNA/product sizes | Sequence (5′-3′) | Amplified length (bp) |
---|---|---|
GAPDH | Forward primers: ggtgaaggtcggtgtgaacg | 233 |
Reverse primers: ctcgctcctggaagatggtg | ||
TRPV1 | Forward primers: gtttacctcgtccaccctga | 106 |
Reverse primers: agagagccatcaccatcctg | ||
P2X3 | Forward primers: tctccagcagagacatcagca | 163 |
Reverse primers: gggagcatcttggtgaactcag |
Western blot analysis
Data analyses
Results
Group | Number | Micturition volume (μL) | Micturition times (times) | Micturition interval (mins) |
---|---|---|---|---|
WT | 10 | 93.03 ± 18.6 | 2.47 ± 0.64 | 104.3 ± 23.7 |
KO | 8 | 116.1 ± 21.7* | 1.92 ± 0.76* | 132.2 ± 32.1* |
KO + SQW 1170 mg/kg | 8 | 101.27 ± 35.6 | 2.07 ± 0.47 | 123.3 ± 32.1 |
KO + SQW 585 mg/kg | 8 | 103.7 ± 43.6 | 2.12 ± 0.51 | 118.4 ± 36.1 |