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01.02.2013 | Original Article

Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: a histological and histometric study in rat calvaria

verfasst von: Valdir Gouveia Garcia, Juliana Mendonça da Conceição, Leandro Araújo Fernandes, Juliano Milanezi de Almeida, Maria José Hitomi Nagata, Alvaro Francisco Bosco, Leticia Helena Theodoro

Erschienen in: Lasers in Medical Science | Ausgabe 2/2013

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Abstract

The purpose of this study was to analyze histologically the effect of low-level laser therapy (LLLT) in combination with bisphosphonate on bone healing in surgically created critical size defects (CSD) in rat calvaria. One hundred Wistar female rats sham operated (sham) and ovariectomized (Ovx) were maintained untreated for 1 month to allow for the development of osteopenia in the Ovx animals. A CSD was made in the calvarium of each rat, and the animals were divided into five groups according to following treatments: (1) sham rats (control), (2) Ovx rats, (3) Ovx rats treated with LLLT, (4) Ovx rats treated with bisphosphonate, and (5) Ovx rats treated with bisphosphonate and LLLT. Groups 4 and 5 were irrigated with 1 ml of bisphosphonate, and groups 3 and 5 were submitted to LLLT (GaAlAs), 660 nm, 24 J, and 0.4285 W/cm² on the CSD. Ten animals of each treatment were killed at 30 and 60 days. Histomorphometric assessments, using image analysis software, and histological analyses were performed. No defect was completely regenerated with the bone. Histometrically, it can be observed that groups 3 (37.49 ± 1.94%, 43.11 ± 2.39%) and 5 (35.05 ± 1.57%, 41.07 ± 1.89%) showed a significant bone neoformation when compared to groups 1 (16.81 ± 1.57%, 27.54 ± 1.49%), 2 (11.68 ± 0.98%, 22.51 ± 1.05%), and 4 (14.62 ± 1.70%, 25.67 ± 1.41%) in all experimental periods (P < 0.05). It was possible to conclude that the LLLT associated or not with bisphosphonate treatment was effective for stimulating bone formation in CSD in the calvaria of rats submitted to ovariectomy.

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Titel: Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: a histological and histometric study in rat calvaria
verfasst von: Valdir Gouveia Garcia
Juliana Mendonça da Conceição
Leandro Araújo Fernandes
Juliano Milanezi de Almeida
Maria José Hitomi Nagata
Alvaro Francisco Bosco
Leticia Helena Theodoro
Publikationsdatum: 01.02.2013
Verlag: Springer-Verlag
Erschienen in: Lasers in Medical Science / Ausgabe 2/2013
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-012-1068-5

Springer Medizin

Abstract

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