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Lasers in Medical Science

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12.05.2020 | Original Article

Effects of photobiomodulation at different wavelengths on orthodontically induced root resorption in orthodontic retention period: a micro-CT and RT-PCR study

verfasst von: Taner Ozturk, Nisa Gul Amuk

Erschienen in: Lasers in Medical Science | Ausgabe 6/2020

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Abstract

The aim of this study was to evaluate and compare the reparative and inhibitory effects of single wavelength photobiomodulation (SW-PBM) and of cumulative increased wavelength photobiomodulation (CW-PBM) on orthodontically induced inflammatory root resorption (OIIRR). Thirty-three Wistar albino rats were divided into five groups: untreated group (negative control), only relapse group (positive control-1), only retention group (positive control-2), SW-PBM group (650 nm, 100 mW/cm²), and CW-PBM group (532–650–940 nm, 100 mW/cm²). Orthodontic tooth movement was induced experimentally in rats for 10 days with an applied force of 50 cN; retention and therapeutic approaches were performed concurrently. At the end of the experiment, maxillary quadrants were prepared for micro-CT analysis and real-time polymerase chain reaction (RT-PCR) analysis. After the Shapiro-Wilk normality test, Kruskal-Wallis test followed post hoc Bonferroni test and paired samples t test was used for statistical evaluation of the data. Resorption lacunae volume (p < 0.001), number of resorption lacunae (p < 0.05), and percentage of the resorption (PR) lacunae (p < 0.001) decreased with PBM applications when compared with the positive control groups, and the mean PR was similar in the negative control group when compared with SW-PBM group. Receptor activator of nuclear factor kappa B ligand (RANKL) levels of the PBM groups were lower (p < 0.05) than those of the positive control groups. Cyclooxygenase-2 (COX-2) expression levels significantly decreased with PBM administration (p < 0.05). No significant change was found in osteoprotegerin (OPG) expression levels and OPG/RANKL ratios (p > 0.05). PBM applications showed marked inhibitory and reparative effects on OIIRR by modulating the RANKL and COX-2 expression levels. However, the effects of the different wavelengths were similar to each other.

Graphical abstract

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Titel: Effects of photobiomodulation at different wavelengths on orthodontically induced root resorption in orthodontic retention period: a micro-CT and RT-PCR study
verfasst von: Taner Ozturk
Nisa Gul Amuk
Publikationsdatum: 12.05.2020
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 6/2020
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03014-1

Springer Medizin

Abstract

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