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14.08.2018 | Original Article

Effects of the extracorporeal shock wave therapy on the skin: an experimental study

verfasst von: Thayná Melo de Lima Morais, Patrícia Froes Meyer, Liliane Santos de Vasconcellos, Julio Costa e Silva, Ito Ferreira e Andrade, Vinicius Alves Freire de Farias, Igor Candido da Silva, Roberta Marinho Falcão Gondim Araújo, Rodrigo Marcel Valentim da Silva, Esteban Fortuny Pacheco, Ciro Dantas Soares

Erschienen in: Lasers in Medical Science | Ausgabe 2/2019

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Abstract

Extracorporeal shock wave therapy (ESWT) has been extensively studied for its multiple biological properties, and although it is widely applied in esthetical procedures, little is known about its effects on the epidermis and dermis. In this study, a histological and immunohistochemical study of the effects of ESWT was performed on rat skin. Forty-five female rats were treated with one or two sessions of ESWT and sacrificed on days 1, 7, 14, and 21 after treatment. The samples were histologically processed and then morphometric analyses were performed to assess the epidermis, dermis, and subcutaneous fat tissue thickness. Immunohistochemical reactions were also performed against the antibodies: basic fibroblastic growth factor (FGF2), its receptor (FGFR1), and α-smooth muscle actin. Slides were scanned and digitally assessed, to determine the microvessel density (MVD) and digital scoring of the immunohistochemical staining. The results showed that ESWT produced a significantly higher collagen content, MVD, and epidermis and dermis thickness than the control, non-treated group. Both in epidermis and dermis, FGF2 was overexpressed in the ESWT-treated groups, whereas FGFR1 was increased only in the group treated with two ESWT sessions at 21-days post-treatment. The ESWT-treated groups have also shown diminished thickness of subcutaneous fat tissue. In conclusion, ESWT induces neocollagenesis and neoangiogenesis, and upregulates the FGF2 expression, particularly in the groups treated with two sessions. Furthermore, it was demonstrated that overexpression of FGF2 on skins treated with ESWT seems to be a key role on its mechanism of action.

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Titel: Effects of the extracorporeal shock wave therapy on the skin: an experimental study
verfasst von: Thayná Melo de Lima Morais
Patrícia Froes Meyer
Liliane Santos de Vasconcellos
Julio Costa e Silva
Ito Ferreira e Andrade
Vinicius Alves Freire de Farias
Igor Candido da Silva
Roberta Marinho Falcão Gondim Araújo
Rodrigo Marcel Valentim da Silva
Esteban Fortuny Pacheco
Ciro Dantas Soares
Publikationsdatum: 14.08.2018
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 2/2019
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-018-2612-8

Springer Medizin

Abstract

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