Our study showed the efficacy and safety of biosimilar Peg-filgrastim in post-transplant engraftment among myeloma and lymphoma patients undergoing ASCT. The median time to neutrophil and platelet engraftment was 10 and 11 days, similarly to that observed in our historical cohorts of patients receiving originator Peg-filgrastim and significantly shorter than those receiving short-acting G-CSFs (i.e., Lenograstim or biosimilar Filgrastim). Pegylated G-CSF was associated with a significantly faster neutrophil engraftment in ASCT in other studies, substantially conducted by using the originator [
17‐
19]. However, several previous reports suggested the comparability for both pharmacokinetic and pharmacodynamic properties between biosimilar Peg-filgrastim and its originator [
20]. Indeed, our study confirmed in the setting of autologous transplant the equivalence in terms of clinical efficacy of both biosimilar and originator Peg-filgrastim, being significantly superior to biosimilar Filgrastim and Lenograstim in terms of neutrophil and platelet engraftment. Data about biosimilar Peg-Filgrastim are still scarce in this context. Recently, some studies have been published, all together showing a slightly superiority of biosimilar pegylated formulations over the short-acting G-CSFs in myeloma and lymphoma patients undergoing ASCT [
11,
21]. The physiological reason for the better performance of pegylated G-CSF formulation can be found looking at the pharmacokinetics of Peg-filgrastim. Indeed, its pharmacological profile allows the same powerful effect on myeloid progenitors with the advantage of a single and fixed-dose injection given per cycle, thanks to reduced renal clearance and extended half-life [
22,
23]; the only pathway of Peg-filgrastim elimination is the neutrophil-mediated clearance [
24]. However, in some studies this biological advantage did not translate in a meaningful better time to neutrophil recovery, mainly because several confounding factors basically due to patient’s selection biases and variability were present in those studies, including population age, CD34 + -infused cells, disease stage, and prior exposure to chemotherapy or radiotherapy. Several factors, other than G-CSFs, are indeed known as able to significantly affect the neutrophil and platelet engraftment after ASCT [
25]. The most relevant are age at transplant, number of CD34 + -infused cells, disease stage, and previous radiant treatment [
25]. In our study, at least two of those factors were able to negatively influence the post-transplant engraftment in biosimilar Peg-Filgrastim patient cohort, since the median age of these patients was significantly higher and they received a significantly lower number of CD34 + . On the contrary, no significant differences were found in terms of previous chemotherapeutic lines and previous radiotherapy. In addition, we carried out a sub-analysis on lymphoma and myeloma patients separately by PSM to generate different pair wise groups with balanced distribution of specific baseline features (age, gender, and number of infused CD34 +). Even in this “unbiased” model, we observed a significant advantage at least in terms of neutrophil engraftment for pegylated G-CSF formulations compared to short-acting ones, confirming the better performance observed in the overall patient population. In our study, we observed a slightly lower incidence of febrile neutropenia episodes and documented infections with consequent lower broad-spectrum antibiotic consumption in patients who received biosimilar Peg-filgrastim; however, it is not statistically significant. This trend is consistent with that reported in the study of Martino et al. [
11] and other groups [
19,
26‐
28], where it however reached the statistical significance. Our data about febrile neutropenia episodes and documented infections could be however potentially influenced by the higher number of myeloma patients observed in the biosimilar Peg-filgrastim group. Indeed, taking a look at lymphoma sub-analysis, we can easily understand that the number of febrile episodes and antibiotic needing was quite similar among all the patient’s cohorts, suggesting that febrile neutropenia was more rarely detected in myeloma patients, irrespective to the received G-CSF formulation, in accordance with our previous published data [
29]. Further studies are warranted to better clarify whether the G-CSF formulation can potentially influence the occurrence of post-transplant febrile neutropenia, documented infections and antibiotic needing in ASCT. As a consequence of a shorter engraftment time, from our study, we observed a significantly lower transfusion needing and a shorter hospitalization time among patients receiving biosimilar and originator Peg-filgrastim. In particular, this difference was statistically significant compared to Lenograstim patient cohort, and less evident if compared to biosimilar Filgrastim. Even if hospitalization duration could be potentially affected by several confounding factors, this datum seems to be relevant in our opinion, since less hospitalization time means a better management of health resources in terms of both organization and costs. In general, it has already been widely discussed and demonstrated in the literature how the lower cost of biosimilar drugs and the consequent savings derived from their use can lift the financial burden of health care systems and increase patient access to drugs [
30‐
32]. Over the past decade, the biosimilar Filgrastim transformed patient access, with clear evidence of clinical benefits in preventing febrile neutropenia at reduced costs (savings conservatively estimated at 39% in Europe) and in 2019 the licensing in Europe of the biosimilar Peg-filgrastim provided the opportunity to offer the additional benefits of long-acting G-CSF over short-acting G-CSF at a reduced cost [
33‐
35]. Our study showed that potential cost savings per patient range from approximately € 1500 to approximately € 5500 by adopting the biosimilar Peg-filgrastim in place of biosimilar Filgrastim, originator Peg-filgrastim or Lenograstim. These benefits could be more substantial at a population level. For example, the economic impact of introducing biosimilar Peg-filgrastim compared to the current standard G-CSF practice in France was estimated to generate a cost saving from € 51007531 to € 287344835 over 5 years switching from the current standard practice to biosimilar Peg-filgrastim [
36]. In Germany, the health-economic impact of biosimilar Peg-filgrastim in the real world for healthcare system would generate a potential annual savings of up to € 56.4 million, with a saving of up to € 4199 per patient compared to originator product [
37]. Most economic evaluations of biosimilars consider only the cost of the drug, but it is fundamental in economic evaluations to estimate the real savings beyond the cost of the drug, especially in the case of differences in the form of administration or in adherence, differences in use of healthcare resources, or to consider value-added services. Our study showed that cost savings of biosimilar Peg-filgrastim were in fact mainly attributable at the inpatient management. A real-world data study on primary prophylaxis with Peg-filgrastim vs Filgrastim in cancer patients at intermediate-to-high risk of febrile neutropenia showed that biosimilar Peg-filgrastim was dominant (with a cost saving of $ 5703 and a gain of 0.28 quality-adjusted life year (QALY)) compared with biosimilar Filgrastim for the high-risk group and a cost/QALY of $ 14502 for the intermediate-risk group [
38]. In line with our study, the main saving of biosimilar Peg-filgrastim vs biosimilar Filgrastim was primarily driven by a lower cost of inpatient febrile neutropenia management for patients receiving biosimilar Peg-filgrastim [
38]. The savings arising from the cost containment using biosimilar Peg-filgrastim could be reallocated to increase patient access to innovative therapies, to move therapy to an earlier line of treatment, to increase the number of healthcare staff thus resulting in a better health outcome for more patients. Peg-filgrastim administration was safe, with no reported grade 3–4 adverse events and the safety profile was similar to that seen for the other G-CSF formulations. At the same time, we observed a superimposable rate of mucositis and diarrhea among the four patient cohorts and the TRM was quite similar, overall ranging between 1 and 4%. This is quite in contrast to some previous report [
11,
39] in which the authors showed a lower incidence of mucositis and grade 2–3 diarrhea in patients who received pegylated G-CSFs both in myeloma patients undergoing ASCT and in breast cancer patients, respectively. Multiple factors can potentially explain this difference, keeping in mind that post-transplant gastrointestinal toxicities are usually related to several variables not always easy to predict and control. The present study has some limitations, basically due to the study design, including the use of historical controls. The differences detected in the baseline demographic and clinical characteristics among the four patient cohorts can potentially affect the reliability of our findings. In addition, we analyzed only a small amount of lymphoma patients who received biosimilar Peg-filgrastim after ASCT. Even in this last group, although we used a PSM to generate different pair-wise groups with balanced distribution of specific baseline features in order to maximize the comparability among the different patient cohorts, our results should be read carefully particularly if we consider the limited sample size. In conclusion, being aware of the limitations discussed above, from our study biosimilar Peg-filgrastim seems to be as effective as the originator and more effective than short-acting G-CSF formulations (Lenograstim and biosimilar Filgrastim) in terms of post-transplant engraftment in myeloma and lymphoma patients undergoing ASCT. In addition, pegylated formulations seem to be associated to a better patient clinical management in terms of transfusion needing, febrile neutropenia, and hospitalization duration. Finally, from our pharmacoeconomic evaluation, biosimilar Peg-filgrastim was cost-effective when compared with the other G-CSF formulations and savings derived from its use may contribute to an expansion of medical treatment options for patients, hence concomitantly contributing to the long-term sustainability of the healthcare system. We believe that our findings could help clinicians and healthcare decision-makers in the better management of febrile neutropenia prophylaxis of myeloma and lymphoma patients undergoing ASCT.