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30.10.2019 | Gynecologic Endocrinology and Reproductive Medicine

Endometrial expression of receptivity markers subject to ovulation induction agents

verfasst von: Alexander Freis, Ariane Germeyer, Julia Jauckus, Edison Capp, Thomas Strowitzki, Markus Zorn, Amanda Machado Weber

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 6/2019

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Abstract

Purpose

Implantation rates differ according to ovulation induction agents in ART. This study investigates the different local endometrial effects of LH- versus hCG-induced ovulation.

Methods

Endometrial stromal cells from healthy patients were cultured with hCG or LH in different concentrations, supplemented with 250 ng/mL hCG and progesterone after 2 and 5 days. In addition after decidualization induction, cells were treated with hCG (50 or 250 ng/mL) or LH (10 or 50 ng/mL) for 3 days. Receptivity markers expression was evaluated by real-time quantitative PCR on day 3 and 6.

Results

On day 3, non-decidualized cells treated with LH showed an increased expression of IGFBP1, IL-8 and CXCL12 compared to hCG. The expression pattern changed on day 6, where cells treated with hCG showed higher expression of implantation markers compared to LH-treated cells. Furthermore, on day 3, decidualized cells treated with hCG250 showed an increased IL8 and CXCL12 expression compared to LH10.

Conclusions

LH seems to modulate the local endometrial expression of receptivity markers earlier compared to hCG; however, the effect is not sustained over time in cells without prior decidualization. Though, in decidualized cells, pattern changed and an earlier positive effect of hCG was shown on IL-8 and CXCL12.

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Titel: Endometrial expression of receptivity markers subject to ovulation induction agents
verfasst von: Alexander Freis
Ariane Germeyer
Julia Jauckus
Edison Capp
Thomas Strowitzki
Markus Zorn
Amanda Machado Weber
Publikationsdatum: 30.10.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 6/2019
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-019-05346-y

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Endometrial expression of receptivity markers subject to ovulation induction agents