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30.10.2017 | Original Article

Enterococcus faecium and Enterococcus faecalis in endodontic infections: antibiotic resistance profile and susceptibility to photodynamic therapy

verfasst von: Ana Carolina Chipoletti Prado, Patrícia Pimentel De Barros, Jéssica Diane Dos Santos, Luciane Dias De Oliveira, Claudio Antônio Talge Carvalho, Marcia Carneiro Valera, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira

Erschienen in: Lasers in Dental Science | Ausgabe 2-4/2017

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Abstract

Introduction

Enterococcus faecium has become an important microorganism in nosocomial infections with great ability to acquire antibiotic resistance. However, little is known about their presence on the oral cavity. Therefore, our objective was to verify the presence of E. faecium and E. faecalis in endodontic infections and compare their susceptibility to conventional antibiotics and photodynamic therapy.

Methods

We performed 38 collections from the root canals of different patients. Positive Enterococcus agar samples were submitted to phenotypic and genotypic testing for species-specific confirmation. The isolates identified as E. faecium and E. faecalis were tested for susceptibility to antibiotics by the E-test method. After that, the isolates were evaluated for susceptibility to photodynamic therapy (PDT) using methylene blue and gallium arsenide aluminum laser with a wavelength of 660 nm and fluence of 39.5 J/cm² (energy of 15 J and time of 428 s).

Results

Cultures positive for E. faecalis were found in 22 patients (58%). Among these patients, only two had E. faecium in mixed infections with E. faecalis. In the isolates of E. faecalis, 27% were resistant to antibiotics, including tetracycline, ciprofloxacin, and azithromycin. The isolates of E. faecium showed no resistance to the antibiotics tested. Both the isolates of E. faecium and E. faecalis exhibit significant susceptibility to PDT, including the isolates resistant to antibiotics. The reductions achieved by PDT ranged of 2.76 to 4.31 log₁₀ for E. faecalis strains and of 3.93 to 4.33 log₁₀ for E. faecium strains.

Conclusion

E. faecium showed lower prevalence in endodontic infections and higher susceptibility to antibiotics when compared to E. faecalis. In in vitro assays, PDT had a significant antimicrobial activity for both strains.

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Titel: Enterococcus faecium and Enterococcus faecalis in endodontic infections: antibiotic resistance profile and susceptibility to photodynamic therapy
verfasst von: Ana Carolina Chipoletti Prado
Patrícia Pimentel De Barros
Jéssica Diane Dos Santos
Luciane Dias De Oliveira
Claudio Antônio Talge Carvalho
Marcia Carneiro Valera
Antonio Olavo Cardoso Jorge
Juliana Campos Junqueira
Publikationsdatum: 30.10.2017
Verlag: Springer International Publishing
Erschienen in: Lasers in Dental Science / Ausgabe 2-4/2017
Elektronische ISSN: 2367-2587
DOI: https://doi.org/10.1007/s41547-017-0011-2

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Enterococcus faecium and Enterococcus faecalis in endodontic infections: antibiotic resistance profile and susceptibility to photodynamic therapy