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10.02.2018 | Original Article

Essential oil from waste leaves of Curcuma longa L. alleviates skin inflammation

verfasst von: Anant Kumar, Karishma Agarwal, Monika Singh, Archana Saxena, Pankaj Yadav, Anil Kumar Maurya, Anju Yadav, Sudeep Tandon, Debabrata Chanda, Dnyaneshwar U. Bawankule

Erschienen in: Inflammopharmacology | Ausgabe 5/2018

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Abstract

Background

Curcuma longa L. is an important industrial crop used by medicinal and cosmetic industries in the world. Its leaves are a waste material after harvesting rhizomes. The aim of the study was to evaluate the chemical and pharmacological profile of essential oil from waste leaves of Curcuma longa (EOCl) against skin inflammation.

Methods

EOCl was subjected to gas chromatography (GC) analysis for identification of essential oil constituents and its anti-inflammatory evaluation through in vitro and in vivo models.

Results

Chemical fingerprinting using GC and GC–MS analysis of EOCl revealed the presence of 11 compounds, representing 90.29% of the oil, in which terpinolene (52.88%) and α-phellandrene (21.13%) are the major components. In the in vitro testing EOCl inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) in lipopolysaccharide (LPS) and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in the human keratinocyte cell line (HaCaT). Topical application of EOCl produced anti-inflammatory effects by reducing ear thickness, ear weight and ameliorating the level of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) at protein and mRNA levels as well as regulating the overproduction of oxidative markers and restoring the histopathological damage in a TPA-induced mouse model of inflammation.

Conclusion

These findings of topical anti-inflammatory properties of EOCl provide a scientific basis for medicinal use of this plant material against inflammatory disorders.

Graphical Abstract

Adams RP (2001) Identification of essential oil components by gas chromatography/mass spectrometry. Allured, Carol Stream

Aroonrerk N, Kamkaen N (2009) Anti-inflammatory activity of Quercus infectoria, Glycyrrhiza uralensis, Kaempferia galangal and Coptis chinensis, the main components of thai herbal remedies for aphthous ulcer. J Health Res 23(1):17–22

Bradley PP, Priebat DA, Christensen RD, Rothstein G (1982) Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol 78:206–209CrossRef

Bralley EE, Greenspan P, Hargrove JL, Wicker L, Hartle DK (2008) Topical anti-inflammatory activity of Polygonum cuspidatum extract in the TPA model of mouse ear inflammation. J Inflamm 5(1):1–7CrossRef

Chung WY, Park JH, Kim MJ, Kim HO, Hwang JK, Lee SK, Park KK (2007) Xanthorrhizol inhibits 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced acute inflammation and two-stage mouse skin carcinogenesis by blocking the expression of ornithine decarboxylase, cyclooxygenase-2 and inducible nitric oxide synthase through mitogen-activated protein kinases and/or the nuclear factor-kB. Carcinogenesis 28(6):1224–1231CrossRef

De Young LM, Kheifets JB, Ballaron SJ, Young JM (1989) Edema and cell infiltration in the phorbol ester-treated mouse ear are temporally separate and can be differentially modulated by pharmacologic agents. Agents Actions 26(3–4):335–341CrossRef

Del Rio D, Stewart AJ, Pellegrini N (2005) A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis 15:316–328CrossRef

Halliwell B, Gutteridge JM (1990) Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol 186:1–85CrossRef

Jain S, Shrivastava S, Nayak S, Sumbhate S (2007) Recent trends in Curcuma Longa Linn. Pharmacogn Rev 1(1):119–128

Jena S, Ray A, Banerjee A, Sahoo A, Nasim N, Sahoo S, Basudeba Kar, Patnaik J, Panda PC, Nayak S (2017) Chemical composition and antioxidant activity of essential oil from leaves and rhizomes of Curcuma angustifolia Roxb. Nat Prod Res 31(18):2188–2191CrossRef

Kim HP, Son KH, Chang HW, Kang SS (2004) Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci 96(3):229–245CrossRef

Kim JY, Park SJ, Yun KJ, Cho YW, Park HJ, Lee KT (2008) Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-kappaB in RAW 264.7 macrophages. Eur J Pharmacol 584(1):175–184CrossRef

Kupper TS, Fuhlbrigge RC (2004) Immune surveillance in the skin: mechanisms and clinical consequences. Nat Rev Immunol 4(3):211–222CrossRef

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25(4):402–408CrossRef

Marklund S, Marklund J (1974) Involvement of superoxide anion radicals in the autoxidation of pyrogallol and a convenient assay of superoxide dismutase. Eur J Biochem 47(1974):469–474CrossRef

Maurya AK, Mohanty S, Pal A, Chanotiya CS, Bawankule DU (2018) The essential oil from Citrus limetta Risso peels alleviates skin inflammation: in-vitro and in-vivo study. J Ethnopharmacol 212:86–94CrossRef

Murphy JE, Robert C, Kupper TS (2000) Interleukin-1 and cutaneous inflammation: a crucial link between innate and acquired immunity. J Invest Dermatol 114(3):602–608CrossRef

Padmaja M, Sravanthi M, Hemalatha KPJ (2011) Evaluation of antioxidant activity of two Indian medicinal plants. J Phytol 3(3):86–91

Reische DW, Lillard DA, Eitenmiller RR (1998) Antioxidants in food lipids. In: Ahoh CC, Min DB (eds) Chemistry, nutrition and biotechnology. Dekker, New York, pp 423–448

Sharma S, Chattopadhyay SK, Yadav DK, Khan F, Mohanty S, Maurya A, Bawankule DU (2012) QSAR, docking and in vitro studies for anti-inflammatory activity of cleomiscosin A methyl ether derivatives. Eur J Pharm Sci 47(5):952–964CrossRef

Singh M, Hamid AA, Maurya AK, Prakash O, Khan F, Kumar A, Aiyelaagbe OO, Negi AS, Bawankule DU (2014) Synthesis of diosgenin analogues as potential anti-inflammatory agents. J Steroid Biochem Mol Biol 143:323–333CrossRef

Srivilai J, Waranuch N, Tangsumranjit A, Khorana N, Ingkaninan K (2017) Germacrone and sesquiterpene-enriched extracts from Curcuma aeruginosa Roxb increase skin penetration of minoxidil, a hair growth promoter. Drug Deliv Transl Res. https://doi.org/10.1007/s13346-017-0447-71-10 CrossRef

Tripathi AK, Prajapati V, Verma N, Bahl JR, Bansal RP, Khanuja SP, Kumar S (2002) Bioactivities of the leaf essential oil of Curcuma Longa (Var. Ch-66) on three species of stored-product beetles (Coleoptera). J Econ Entomol 95:183–189CrossRef

Trouba KJ, Hamadeh HK, Amin RP, Germolec DR (2002) Oxidative stress and its role in skin disease. Antioxid Redox Signal 4:665–673CrossRef

Verma AK, Wheeler DL, Aziz MH, Manoharan H (2006) Protein kinase and development of squamous cell carcinoma, the nonmelanoma human skin cancer. Mol Carcinog 45:381–388CrossRef

Wilmer JL, Burleson FG, Kayama F, Kanno J, Luster MI (1994) Cytokine induction in human epidermal keratinocytes exposed to contact irritants and its relation to chemicalinduced inflammation in mouse skin. J Invest Dermatol 102(6):915–922CrossRef

Yadav DK, Mudgal V, Agrawal J, Maurya AK, Bawankule DU, Chnotiya CS, Khan F, Thul ST (2013) Molecular docking and ADME studies of natural compounds of Agarwood oil for topical anti-inflammatory activity. Curr Comput Aided Drug Des 9(3):360–370CrossRef

Young CN, Koepke JI, Terlecky LJ, Borkin MS, Boyd Savo L, Terlecky SR (2008) Reactive oxygen species in tumor necrosis factor-aactivated primary human keratinocytes: implications for psoriasis and inflammatory skin disease. J Invest Dermatol 128:2606–2614CrossRef

Titel: Essential oil from waste leaves of Curcuma longa L. alleviates skin inflammation
verfasst von: Anant Kumar
Karishma Agarwal
Monika Singh
Archana Saxena
Pankaj Yadav
Anil Kumar Maurya
Anju Yadav
Sudeep Tandon
Debabrata Chanda
Dnyaneshwar U. Bawankule
Publikationsdatum: 10.02.2018
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 5/2018
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-018-0447-3

Springer Medizin

Abstract

Background

Methods

Results

Conclusion

Graphical Abstract

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