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Clinical Oral Investigations

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14.04.2022 | Original Article

Evaluation of salivary and serum methylated arginine metabolites and nitric oxide synthase in advanced periodontitis patients

verfasst von: Volkan Şengül, Zeliha Güney, Şivge Kurgan, Canan Önder, Muhittin A. Serdar, Meral Günhan

Erschienen in: Clinical Oral Investigations | Ausgabe 7/2022

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Abstract

Objectives

Methylated arginine metabolites and nitric oxide synthase (NOS) play a critical role in regulating endothelial function. The aim of this study was to determine levels of NOS, and methylated arginine metabolites (ADMA, SDMA, homoarginine, arginine, and L-NMMA) and IL-6 in serum and saliva in patients with advanced periodontal diseases and identify their association with clinical parameters.

Materials and methods

The study consisted of two groups: healthy individuals (control: n = 24), and generalized Stage III Grade B periodontitis (P: n = 21). Clinical periodontal parameters (probing pocket depth, bleeding on probing, clinical attachment level) were recorded. IL 6 and NOS levels in saliva and serum were analyzed by enzyme-linked immunosorbent assay (ELISA). ADMA, SDMA, homoArg, arginine, and L-NMMA in saliva and serum were analyzed by liquid chromatography–mass spectrometry (LC MS/MS).

Results

Clinical parameters were significantly higher in the periodontitis group (p < 0.001). In periodontitis group, NOS, ADMA, and arginine levels in saliva were statistically significantly higher than control group (p < 0.05). Serum levels of SDMA were statistically significantly lower, and IL-6 was statistically significantly higher in P group than C group (p < 0.05). ADMA, NOS, and arginine levels were significantly positive correlated with all clinical periodontal parameters (p < 0.05).

Conclusions

These findings suggest that there is a relationship between severity of periodontal disease and endothelial dysfunction by means of ADMA. Salivary ADMA may be related with periodontal inflammation.

Clinical relevance

ADMA levels in periodontal inflammation are associated with endothelial dysfunction. According to the results of our study, periodontal inflammation is effective on both local and systemic methylated arginine metabolites and nitric oxide synthase levels. This may shed light on the relationship between periodontal disease and systemic status.

William’s probe, Hu-Friedy, Chicago, IL.

Human IL-6 ELISA Kit, Uscn, Cloud-Clone Corp, USA and Human NOS1 ELISA Kit, Uscn, Cloud-Clone Corp, USA.

Synergy HT Microplate Reader, Bio-Tek Instruments, Winooski, WT, USA.

TSQ Quantum Access MAX Triple Stage Quadrupole Mass, Thermo Scientific, USA.

3.1.9.2 G*Power; https://www.psychologie.hhu.de/arbeitsgruppen/allgemeine-psychologie-undarbeitspsychologie/gpower.html.

SPSS for Windows v.26, IBM SPSS Inc., New York, NY, USA.

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Titel: Evaluation of salivary and serum methylated arginine metabolites and nitric oxide synthase in advanced periodontitis patients
verfasst von: Volkan Şengül
Zeliha Güney
Şivge Kurgan
Canan Önder
Muhittin A. Serdar
Meral Günhan
Publikationsdatum: 14.04.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 7/2022
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-022-04479-w

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Evaluation of salivary and serum methylated arginine metabolites and nitric oxide synthase in advanced periodontitis patients

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Neu im Fachgebiet Zahnmedizin

Endlich: Zi zeigt, mit welchen PVS Praxen zufrieden sind

Parodontalbehandlung verbessert Prognose bei Katheterablation

Invasive Zahnbehandlung: Wann eine Antibiotikaprophylaxe vor infektiöser Endokarditis schützt

Zell-Organisatoren unter Druck: Mechanismen des embryonalen Zahnwachstums aufgedeckt

Newsletter

Live-Webinar "Chronische Unterbauch- und Viszeralschmerzen in der Praxis managen"

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Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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