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Lasers in Medical Science

Erschienen in:

01.05.2014 | Original Article

Evidence for A₁ and A₃ receptors mediating adenosine-induced intracellular calcium release in the dorsal root ganglion neurons by using confocal microscopy imaging

verfasst von: Liqin Zheng, Jiangxu Chen, Yimei Huang, Yuhua Wang, Hongqin Yang, Yanding Zhang, Shusen Xie

Erschienen in: Lasers in Medical Science | Ausgabe 3/2014

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Abstract

Adenosine exerts a key role in analgesia. In the present study, adenosine-induced Ca²⁺ responses were revealed by using confocal microscopy imaging in the rat dorsal root ganglia (DRG) neurons in vitro. Our results showed that adenosine could evoke increases in the intracellular Ca²⁺ concentration in the DRG neurons. In addition, by application of selective receptor antagonists, two types of receptors, A₁R and A₃R, were identified to be involved in the adenosine-induced Ca²⁺ release from intracellular stores in neurons. Altogether, these results suggest that confocal microscopy imaging combined with fluorescent dyes could help to detect the analgesic-induced ion signaling in single cell.

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Titel: Evidence for A1 and A3 receptors mediating adenosine-induced intracellular calcium release in the dorsal root ganglion neurons by using confocal microscopy imaging
verfasst von: Liqin Zheng
Jiangxu Chen
Yimei Huang
Yuhua Wang
Hongqin Yang
Yanding Zhang
Shusen Xie
Publikationsdatum: 01.05.2014
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 3/2014
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-013-1511-2

Springer Medizin

Abstract

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