Background
Clinical significance
Methods
Patients
Age (years) | |
---|---|
Average | 63.2 |
Median | 51 |
Gender | |
Male | 37 (66.0%) |
Female | 19 (33.9%) |
Parcial Nephrectomy | 16 (28.6%) |
Radical Nephrectomy | 40 (71.4%) |
Fuhrman’s Grade | |
1–2 | 32 (57.2%) |
3–4 | 24 (42.8%) |
Microvascular Invasion | |
Absent | 44 (78.6%) |
Present | 12 (21.4%) |
Pathological Stage (T) | |
pT1-T2 | 26 (46.4%) |
pT3-T4 | 30 (53.6%) |
Pathological Stage (N) | |
N0 | 54 (96.4%) |
N1 | 02 (3.6%) |
Pathological Stage (M) | |
MO | 48 (85.7%) |
M1 | 08 (14.3%) |
Storage of samples
miRNA isolation of RNA and cDNA synthesis
cDNA synthesis
Analysis of miRNA and RNA expression
Statistical analysis
Results
ccRCC angiogenesis gene and miRNA expression profile
mTOR | VEGFR1 | VEGFR2 | VEGFA | HIF1A | PDGF | VHL | |
---|---|---|---|---|---|---|---|
Low (n = 18) | 0.63(1.32) | 1.96(2.10) | 3.17(6.12) | 11.87 (16.14) | 1.56 (2.27) | 5.91 (11.18) | 0.29 (1.04) |
Intermediate (n = 29) | 1.32 (5.43) | 1.84(4.85) | 1.11(1.38) | 7.10 (4.47) | 1.05 (1.29) | 4.27 (9.39) | 18.28 (87.65) |
High n = 9 | 0.55 (0.63) | 4.48(6.10) | 1.51(2.26) | 4.20 (4.58) | 3.12 (5.58) | 7.05 (9.53) | 5.18 (6.73) |
p | 0.82b
| 0.37b
| 0.89b
| 0.18b
| 0.43b
| 0.76b
| 0.67b
|
Size ≥7 cm (n = 30) | 0.52 (1.11) | 1.74 (2.06) | 2.41 (5.14) | 10.33 (13,53) | 1.27 (1.97) | 6.62 (12.76) | 0.39 (1.20) |
Size <7 cm (n = 26) | 1.54 (5.84) | 2.69 (5.81) | 1.31 (1.72) | 6.32 (4.45) | 1.74 (3.10) | 3.56 (5.17) | 18.84 (85.70) |
p | 0.97a
| 0.59a
| 0.87a
| 0.17a
| 0.50a
| 0.97a
| 0.12a
|
MVI absent (n = 44) | 0.45 (0.95) | 2.03(4.11) | 2.08 (4.27) | 9.77 (11.28) | 1.10(1.63) | 5.24(10.57) | 12.54(72.60) |
MVI present (n = 12) | 3.39 (9.18) | 2.75(4.76) | 1.10 (1.71) | 3.61(3.57) | 2.91(4.36) | 4.89(7.41) | 3.66(5.73) |
p | 0.25a
| 0.61a
| 0.46a
| 0.57a
| 0.08a
| 0.91a
| 0.68a
|
Fuhrman’s Grade I e II (n = 32) | 1.43 (5.32) | 1.57 (1.79) | 2.43 (4.97) | 9.86 (13.09) | 1.54 (2.11) | 4.39 (9.21) | 0.19 (0.84) |
Fuhrman’s Grade III e IV (n = 24) | 0.40 (0.52) | 3.00 (6.10) | 1.19 (1.68) | 6.57 (4.81) | 1.45 (3.06) | 6.17 (10.97) | 20.65 (89.28) |
p | 0.81a
| 0.45a
| 0.56a
| 0.26a
| 0.89a
| 0.51a
| 0.13a
|
Metastasis Absent (n = 48) | 0.46(0.93) | 1.81 (2.86) | 1.96 (4.14) | 8.53 (11.20) | 1.54(2.76) | 5.59 (10.78) | 1.18 (3.44) |
Metastasis Present (n = 8) | 3.64(9.71) | 4.03 (8.24) | 1.41 (2.10) | 7.98(4.54) | 1.27(1.20) | 3.05(3.04) | 54.33(151.70) |
p | 0.82a
| 0.49a
| 0.71a
| 0.89a
| 0.77a
| 0.49a
| 0.79a
|
pT1–2 (n = 26) | 0.51 (1.16) | 1.33 (1.74) | 2.47 (5.31) | 10.12 (14.05) | 1.47 (2.13) | 7.07 (13.16) | 0.20 (0.86) |
pT3–4 (n = 30) | 1.48 (5.62) | 3.03 (5.63) | 1.33 (1.72) | 6.83 (4.67) | 1.53 (2.95) | 3.34 (4.95) | 19.00 (85.67) |
p | 0.38a
| 0.51a
| 0.97a
| 0.26a
| 0.80a
| 0.16a
| 0.18a
|
ccRCC angiogenesis gene and miRNA expression profile and prognostic factor
miR-199a | miR-100 | miR-99a | miR-99b | miR-126 | miR-29a | miR-29b | |
---|---|---|---|---|---|---|---|
Low (n = 18) | 60.12(104.18) | 0.51(1.34) | 17.89(34.38) | 22.01(37.5) | 2.10(4.1) | 19.25(28.23) | 3.94(7.35) |
Intermediate (n = 29) | 59.99(159.22) | 5.62(25.37) | 22.18 (49.15) | 13.07(31.1) | 0.96(2.9) | 10.90(28.49) | 2.72(2.89) |
High n = 9 | 48.64(85.19) | 0.77(1.75) | 44.44(75.01) | 65.13(159.) | 0.37(0.3) | 7.05(9.72) | 5.30(2.91) |
P | 0.65b
| 0.63b
| 0.50b
| 0.17b
| 0.29b
| 0.57b
| 0.47b
|
Size ≥7 cm (n = 30) | 61.13(91.26) | 5.69(26.31) | 19.77(39.93) | 22.79(42.3) | 1.57(3.6) | 13.80(24.88) | 3.48(6.21) |
Size <7 cm (n = 26) | 55.80(166.90) | 0.90(2.77) | 27.68(56.40) | 23.59(84.3) | 1.02(2.1) | 12.62(28.97) | 3.29(2.90) |
P | 0.88a
| 0.36a
| 0.57a
| 0.96a
| 0.49a
| 0.88a
| 0.12a
|
MVI absent (n = 44) | 63.00(145.83) | 3.90(20.87) | 15.77(34.2) | 17.73(35.1) | 1.55(3.2) | 15.24(29.69) | 3.19(5.13) |
MVI present (n = 12) | 43.28(73.27) | 0.88(1.80) | 51.97(76.99) | 43.48(127.) | 0.36(0.3) | 5.37(7.45) | 4.30(3.50) |
P | 0.65a
| 0.63a
| 0.05a
| 0.72a
| 0,43a
| 0.33a
| 0.54a
|
Fuhrman’s Grade I e II (n = 32) | 39.95(87.23) | 0.49(1.32) | 23.61(45.13) | 17.40(31.4) | 1.92(3.7) | 19.08(34.41) | 3.63(6.04) |
Fuhrman’s Grade III e IV (n = 24) | 79.35(169.23) | 6.35(26.88) | 23.28(52.73) | 29.41(88.9) | 0.55(1.2) | 5.70(7.80) | 3.11(3.06) |
p | 0.28a
| 0.33a
| 0.98a
| 0.51a
|
0.03
a
| 0.10a
| 0,71a
|
pT1–2 (n = 26) | 50.88(82.18) | 6.36(27.40) | 22.79(42.04) | 23.35(43.8) | 1.19(2.7) | 14.85(25.19) | 3.86(6.32) |
pT3–4 (n = 30) | 65.37(166.50) | 0.69(2.59) | 24.12(53.93) | 23.01(81.1) | 1.41(3.1) | 11.84(28.52) | 2.96(3.03) |
P | 0.69a
| 0.28a
| 0.92a
| 0.98a
| 0.78a
| 0.71a
| 0.51a
|
Metastasis Absent (n = 48) | 49.76(84.62) | 3.46(19.85) | 19.01(39.85) | 25.78(69.88) | 1.17(2.54) | 12.23(26.81) | 3.61(418.33) |
Metastasis Present (n = 8) | 107.88(286.69) | 1.89(4.94) | 48.92(81.75) | 6.41(6.35) | 2.20(5.18) | 18.95(28.74) | 2.24(3.24) |
p | 0.25a
| 0.83a
| 0.12a
| 0.47a
| 0.39a
| 0.57a
| 0.46a
|
miR-200b | miR-106a | miR-106b | |
---|---|---|---|
Low (n = 18) | 43.39(89.17) | 65.14(65.16) | 159.44(171.94) |
Intermediate (n = 29) | 58.51(98.63) | 110.15(141.57) | 90.68(85.67) |
High n = 9 | 563.01(1128.21) | 202.94(270.20) | 123.81(100.46) |
P |
0.01
b
| 0.23b
| 0.31b
|
Size ≥7 cm (n = 30) | 57.91(97.01) | 79.23(68.51) | 125.34(140.61) |
Size <7 cm (n = 26) | 162.81(545.59) | 139.51(196.72) | 102.81(92.03) |
P | 0.36a
| 0.766 | 0.58a
|
MVI absent (n = 44) | 55.97(95.09) | 93.38(127.07) | 119.47(126.44) |
MVI present (n = 12) | 355.35(899.73) | 173.31(213.58) | 94.63(90.02) |
p | 0.53a
| 0.17a
| 0.17a
|
Fuhrman’s Grade I e II (n = 32) | 56.44(99.57) | 103.91(158.18) | 135.37(152.50) |
Fuhrman’s Grade III e IV (n = 24) | 169.48(557.25) | 113.84(143.83) | 101.01(92.55) |
P | 0.33a
| 0.83a
| 0.40a
|
pT1–2 (n = 26) | 48.99(94.76) | 67.86(60.49) | 115.03(103.12) |
pT3–4 (n = 30) | 161.87(521.68) | 143.33(187.99) | 115.59(136.84) |
P | 0.33a
| 0.11a
| 0.98a
|
Metastasis Absent (n = 48) | 119.99(0.47) | 117.25(5.12) | 109.83(122.37) |
Metastasis Present (n = 8) | 50.56(114.07) | 54.21(43.87) | 149.38(113.15) |
p | 0.66a
| 0.38a
| 0.50a
|