Background
Methods
Study design
Subjects
Non OCP group | OCP group | p-value | |
---|---|---|---|
Number | n = 57 | n = 45 | |
Age (years) | 30 [23.9-35.0] | 28 [22.0-35.6] | NS |
BMI (kg/m2) | 23 [18.5-33.1] | 23 [18.4-32.8] | NS |
AFC (n) | 38 [25.9-74.3] | 38 [26.4-83.0] | NS |
Testosterone (ng/mL) | 0.37 [0.18-0.81] | 0.37 [0.13-0.77] | NS |
Δ4 androstenedione (ng/mL) | 1.76 [0.89-2.83] | 1.84 [0.80-3.01] | NS |
FSH (IU/L) | 6.2 [3.7-8.9] | 5.9 [3.9-8.2] | NS |
AMH (pmol/mL) | 52.2 [18.2-132.5] | 57.2 [14.9-138.9] | NS |
PCOS | 52.6% (n = 30) | 66.7% (n = 30) | NS |
IVF/ICSI indications | -male infertility: 66% | -male infertility: 69% | NS |
-tubal infertility: 16% | -tubal infertility:11% | ||
-ovulation induction failure: 18% | -ovulation induction failure: 20% | ||
ICSI | 66.7% (n = 38) | 68.8% (n = 31) | NS |
Controlled ovarian hyperstimulation protocol
Hormonal assays and ultrasound examination
Statistical analysis
Results
Ultrasonographic and hormonal data
Non OCP group | OCP group | p-value | ||
---|---|---|---|---|
Desensitization day
| <6 mm follicles | 29 [15.2-54.3] | 26 [15.7-65.8] | NS |
6–9 mm follicles | 5 [1.0-14.4] | 6.5 [2.0-18.6] | NS | |
LH (IU/L)
|
1.7 [0.6-4.3]
|
0.7 [0.5-2.5]
|
P = 0.0001
| |
Prog (ng/mL) | 0.2 [0.1-0.6] | 0.3 [0.1-0.6] | NS | |
Testo (ng/mL) | 0.19 [0.05-0.45] | 0.23 [0.06-1.06] | NS | |
Δ4 (ng/mL) | 1.71 [0.86-3.13] | 1.48 [0.74-5.40] | NS | |
D7
| 6–9 mm follicles | 11 [2.1-30.1] | 10 [3.9-40.5] | NS |
≥10 mm follicles | 6 [1.1-17.0] | 6 [1.0-23.0] | NS | |
E2 (pg/mL) | 396 [76.4-1269] | 287 [94.7-1680.9] | NS | |
LH (IU/L) | 0.9 [0.5-2.4] | 0.7 [0.3-1.1] | NS | |
Prog (ng/mL) | 0.3 [0.1-0.6] | 0.3 [0.2-0.5] | NS | |
Testo (ng/mL) | 0.27 [0.10-0.49] | 0.32 [0.05-0.55] | NS | |
Δ4 (ng/mL) | 2.01 [0.87-4.03] | 2.05 [0.82-4.17] | NS | |
HCG-D
| 10–12 mm follicles | 7 [3.0-13.9] | 4 [1.0-15.0] | NS |
13–15 mm follicles | 7 [2.0-23.8] | 7 [2.0-18.2] | NS | |
>15 mm follicles | 8 [4.5-15.5] | 9 [3.0-15.0] | NS | |
E2 (pg/mL) | 3135 [1704.0-5528.1] | 3131 [1120.5-5859.0] | NS | |
LH (IU/L) | 1.4 [0.5-2.2] | 1.0 [0.4-2.3] | NS | |
Prog (ng/mL) | 0.8 [0.3-2.3] | 0.7 [0.2-1.4] | NS | |
Testo (ng/mL) | 0.46 [0.31-1.34] | 0.53 [0.27-1.39] | NS | |
Δ4 (ng/mL) | 3.50 [1.78-7.81] | 3.69 [1.02-6.72] | NS |
Non OCP group | OCP group | p-value | ||
---|---|---|---|---|
Clinical outcome
| Cycles (n) | 57 | 45 | |
% cancellation before retrieval | 15.8% (n = 9) | 13.3% (n = 6) | NS | |
% oocytes retrieval (n) | 84.2% (n = 48) | 86.7% (n = 39) | NS | |
r-FSH starting dose (IU) | 125 [100–200] | 125 [100–150] | NS | |
Duration of stimulation (days) | 11 [8.45-15.55] | 11 [9.0-14.0] | NS | |
Total dose of r-FSH (IU) | 1437.5 [872.5-2555] | 1462.5 [1000–2250] | NS | |
% OHSS | 16.6% (n = 8) | 10.2% (n = 4) | NS | |
Days of GnRH agonist (n)
|
25 [20–33]
|
24 [21–29]
|
P = 0.011
| |
Endometrium thickness
|
D1 (mm)
|
2.0 [2.0-5.8]
|
3.0 [2.0-6.3]
|
P = 0.001
|
HCG-D (mm)
|
11.0 [8.5-14.8]
|
10.0 [7.9-13.0]
|
P = 0.002
| |
Delta (mm) (HCG-D – D1)
|
8.0 [4.4-12]
|
6.5 [2.3-10.3]
|
P < 0.0001
| |
Oocytes
| Total oocytes (n) | 14 [7.5-24.0] | 14 [7.0-27.0] | NS |
Lysed oocytes (n) | 1 [0–5.6] | 2 [0–6.0] | NS | |
Meta II oocytes (n) | 11 [4.0-19.0] | 8 [5.0-22.0] | NS | |
Ratio meta II oocytes/≥ 15 mm follicles | 1.44 (+/−0.92) | 1.32 (+/−0.80) | NS | |
Embryos
| Embryos (n) | 6 [0.5-14.5] | 5 [0.0-13.0] | NS |
Fertilization rate | 63% (+/−21) | 56% (+/−26) | NS | |
“Top quality” embryos (n) | 2 [0.0-7.0] | 1 [0.0-8.0] | NS | |
Cycles with embryo transfer (n) | 46 | 33 | ||
One embryo transferred (%) | 6.5 (n = 3) | 18.2 (n = 6) | NS | |
Two embryos transferred (%) | 87 (n = 42) | 81.8 (n = 27) | NS | |
Three embryos transferred (%) | 2.2 (n = 1) | 0 (n = 0) | NS | |
Cycles with freezing (%) | 58.3 (n = 28) | 51.2 (n = 20) | NS | |
Frozen embryos (n) | 4 [1.0-11.0] | 3.5 [1.0-10.0] | NS | |
Recovery rate after thawing | 72,8% | 77,9% | NS | |
Frozen-thawed embryo transfers (n) | 19 | 21 | ||
Clinical pregnancy rate per frozen-thawed embryo transfer | 31,6% (n = 6) | 38% (n = 8) | NS | |
Ongoing pregnancy rate per frozen-thawed embryo transfer | 31,6% (n = 6) | 38% (n = 8) | NS |