Background
Methods
Mosquito collections and species identification
Microsatellite analysis
Data analysis
Results
Species and molecular form identification
Within population genetic variability
Locus | Annobón [M] Far-island (24) | Malabo [M] Near-island (36) | Sácriba [S] Near-island (35) | Bata [M] Mainland (28) | Ngonamanga [M] Mainland (45) | Libreville [S] Mainland (45) | All Samples (213) | |
---|---|---|---|---|---|---|---|---|
Ag3H242 |
R
s
| 3 | 5 | 7 | 4 | 6 | 5 | 5 |
H
e
| 0.613 | 0.654 | 0.635 | 0.634 | 0.679 | 0.646 | 0.644 | |
Ag3H128 |
R
s
| 4 | 10 | 10 | 16 | 17 | 7 | 11 |
H
e
| 0.659 | 0.849 | 0.721 | 0.919 | 0.921 | 0.666 | 0.789 | |
Ag3H249 |
R
s
| 5 | 6 | 8 | 8 | 7 | 7 | 7 |
H
e
| 0.702 | 0.811 | 0.789 | 0.835 | 0.795 | 0.792 | 0.787 | |
Ag3H119 |
R
s
| 2 | 6 | 10 | 5 | 7 | 9 | 7 |
H
e
| 0.190 | 0.688 | 0.839 | 0.745 | 0.738 | 0.826 | 0.671 | |
Ag3H555 |
R
s
| 1 | 6 | 7 | 7 | 5 | 6 | 5 |
H
e
| - | 0.763 | 0.748 | 0.738 | 0.614 | 0.785 |
0.608
| |
Ag3H577 |
R
s
| 2 | 5 | 5 | 4 | 5 | 5 | 4 |
H
e
| 0.386 | 0.569 | 0.626 | 0.536 | 0.611 | 0.509 | 0.540 | |
Ag3H59 |
R
s
| 4 | 7 | 10 | 7 | 7 | 7 | 7 |
H
e
| 0.727 | 0.652 | 0.873 | 0.705 | 0.763 | 0.746 | 0.744 | |
Ag3H88 |
R
s
| 3 | 10 | 7 | 10 | 10 | 6 | 8 |
H
e
| 0.519 |
0.840
| 0.801 | 0.838 | 0.871 |
0.731
|
0.767
| |
Ag3H758 |
R
s
| 2 | 11 | 9 | 12 | 13 | 7 | 9 |
H
e
| 0.500 | 0.882 | 0.787 | 0.886 |
0.896
| 0.669 |
0.770
| |
Ag3H93 |
R
s
| 2 | 5 | 7 | 7 | 7 | 12 | 7 |
H
e
| 0.504 | 0.757 |
0.675
| 0.731 | 0.718 | 0.862 |
0.708
| |
45C1 |
R
s
| 1 | 5 | 5 | 5 | 4 | 5 | 4 |
H
e
| - | 0.701 | 0.651 | 0.722 | 0.694 | 0.593 | 0.560 | |
All loci |
R
s
| 3 | 7 | 8 | 8 | 8 | 7 | 7 |
H
e
| 0.436 |
0.742
|
0.740
|
0.754
|
0.755
| 0.711 |
0.690
| |
N = 24 |
R
s
| 3 | 8 | 7 | 8 | 7 | 7 | 9 |
H
e
| 0.436 |
0.768
|
0.744
|
0.763
| 0.740 | 0.706 |
0.693
|
SMM | TPM (90%) | TPM (80%) | TPM (70%) | ||
---|---|---|---|---|---|
Annobón [M] (far-island) | H
e
> H
eq
| 8 | 8 | 8 | 8 |
P
(He > Heq)
|
0.005
|
0.003
|
0.002
|
0.002
| |
Malabo [M] (near-island) | H
e
> H
eq
| 3 | 6 | 7 | 8 |
P
(He > Heq)
| 0.913 | 0.517 | 0.289 | 0.160 | |
Sácriba [S] (near-island) | H
e
> H
eq
| 1* | 2* | 3 | 4 |
P
(He > Heq)
| 1.000 | 0.998 | 0.991 | 0.926 | |
Bata [M] (mainland) | H
e
> H
eq
| 4* | 4 | 4 | 5 |
P
(He > Heq)
| 0.992 | 0.794 | 0.585 | 0.382 | |
Ngonamanga [M] (mainland) | H
e
> H
eq
| 2* | 2 | 5 | 5 |
P
(He > Heq)
| 0.998 | 0.966 | 0.768 | 0.740 | |
Libreville [S] (mainland) | H
e
> H
eq
| 1* | 2* | 4 | 5 |
P
(He > Heq)
| 1.000 | 0.995 | 0.912 | 0.768 |
Genetic differentiation and isolation by distance
Annobón | Malabo | Sácriba | Bata | Ngonamanga | |
---|---|---|---|---|---|
Annobón [M] (far-island) | - | ||||
Malabo [M] (near-island) |
0.212
| - | |||
Sácriba [S] (near-island) |
0.269
|
0.089
| - | ||
Bata [M] (mainland) |
0.196
|
0.023
|
0.080
| - | |
Ngonamanga [M] (mainland) |
0.187
|
0.042
|
0.079
| 0.003 | - |
Libreville [S] (mainland) |
0.267
|
0.109
|
0.050
|
0.100
|
0.099
|
Annobón [M] | Malabo [M] | Sácriba [S] | Bata [M] | Ngonamanga [M] | Libreville [S] | |
---|---|---|---|---|---|---|
Annobón [M] (far-island) |
0.88
|
-
|
-
| 0.12 |
-
|
-
|
Malabo [M] (near-island) |
-
|
0.44
| 0.08 | 0.42 | 0.06 |
-
|
Sácriba [S] (near-island) |
-
|
-
|
0.89
| 0.03 | 0.05 | 0.03 |
Bata [M] (mainland) |
-
|
-
| 0.07 |
0.82
| 0.11 |
-
|
Ngonamanga [M] (mainland) |
-
|
-
| 0.16 | 0.57 |
0.27
|
-
|
Libreville [S] (mainland) |
-
|
-
| 0.31 |
-
|
-
|
0.69
|