nach oben

Clinical Oral Investigations

Erschienen in:

24.07.2017 | Original Article

Glass ionomer cement inhibits secondary caries in an in vitro biofilm model

verfasst von: Norbert Krämer, Miriam Schmidt, Susanne Lücker, Eugen Domann, Roland Frankenberger

Erschienen in: Clinical Oral Investigations | Ausgabe 2/2018

Einloggen, um Zugang zu erhalten

Abstract

Objectives

The objective of this study was to investigate the effect of different glass ionomer cements on secondary caries inhibition in a fully automated in vitro biofilm model.

Materials and methods

One hundred and twenty-four extracted third molars received class V cavities and were filled with one conventional (Ketac Molar/KM), and two resin-modified glass ionomer cements (Photac Fil/PF, Ketac N100/KN, 3M Espe). A bonded resin composite (Single Bond Plus/Filtek Supreme XTE) served as control. After 14 days water storage at 37 °C, specimens were thermocycled (10,000 × 5/55 °C). Over a period of 10 days, specimens were subjected to cariogenic challenge for 3/4/6 h/day. Demineralization was caused by Streptococcus mutans (DSM 20523) alternatingly being rinsed over specimens using artificial saliva. After biological loading, teeth were cut longitudinally and demineralization depths were evaluated at the margins and at a distance of 0.5 mm from the margins using fluorescence microscopy. Marginal quality was investigated under a SEM at ×200 magnification.

Results

Four-hour demineralization depths were for enamel margins (EM), enamel (E), dentin margin (DM), and dentin (D) (μm ± SD): KM: EM 12 ± 8, E 33 ± 7, DM 56 ± 11, D 79 ± 6; PF: EM 19 ± 13, E 34 ± 13, DM 53 ± 10, D 77 ± 12; and KN: EM 26 ± 5, E 38 ± 6, DM 57 ± 11, D 71 ± 7. For all glass ionomer cements (GICs), demineralization depth at the margins was less compared to 0.5 mm distance, with demineralization depth having been correlated to duration of cariogenic challenge (ANOVA [mod. LSD, p < 0.05]). Compared to the bonded resin composite, all GICs exhibited caries inhibition at restoration margins in enamel and dentin.

Conclusions

Fluoride-releasing GIC materials exhibit a secondary caries inhibiting effect in vitro.

Clinical relevance

Glass ionomer cements have a higher secondary caries inhibiting effect than resin composites.

Frankenberger R, Reinelt C, Kramer N (2014) Nanohybrid vs. fine hybrid composite in extended class II cavities: 8-year results. Clin Oral Investig 18:125–137CrossRefPubMed

Hickel R, Manhart J, Garcia-Godoy F (2000) Clinical results and new developments of direct posterior restorations. Am J Dent 13:41D–54DPubMed

Manhart J, Chen H, Hamm G, Hickel R (2004) Buonocore Memorial Lecture. Review of the clinical survival of direct and indirect restorations in posterior teeth of the permanent dentition. Oper Dent 29:481–508PubMed

Opdam NJ, van de Sande FH, Bronkhorst E, Cenci MS, Bottenberg P, Pallesen U, Gaengler P, Lindberg A, Huysmans MC, van Dijken JW (2014) Longevity of posterior composite restorations: a systematic review and meta-analysis. J Dent Res 93:943–949CrossRefPubMedPubMedCentral

Barata JS, Casagrande L, Pitoni CM, de Araujo FB, Garcia-Godoy F, Groismann S (2012) Influence of gaps in adhesive restorations in the development of secondary caries lesions: an in situ evaluation. Am J Dent 25:244–248PubMed

Da Rosa Rodolpho PA, Donassollo TA, Cenci MS et al (2011) 22-year clinical evaluation of the performance of two posterior composites with different filler characteristics. Dent Mater 27:955–963CrossRefPubMed

Frankenberger R, Kramer N, Lohbauer U, Nikolaenko SA, Reich SM (2007) Marginal integrity: is the clinical performance of bonded restorations predictable in vitro? J Adhes Dent 9(Suppl 1):107–116PubMed

Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas M, Vijay P, Van Landuyt K, Lambrechts P, Vanherle G (2003) Buonocore memorial lecture. Adhesion to enamel and dentin: current status and future challenges. Oper Dent 28:215–235PubMed

Cenci MS, Pereira-Cenci T, Cury JA, Ten Cate JM (2009) Relationship between gap size and dentine secondary caries formation assessed in a microcosm biofilm model. Caries Res 43:97–102CrossRefPubMed

10.

Demarco FF, Correa MB, Cenci MS, Moraes RR, Opdam NJ (2012) Longevity of posterior composite restorations: not only a matter of materials. Dent Mater 28:87–101CrossRefPubMed

11.

Dennison JB, Sarrett DC (2012) Prediction and diagnosis of clinical outcomes affecting restoration margins. J Oral Rehabil 39:301–318CrossRefPubMed

12.

Krämer N, Kunzelmann KH, Garcia-Godoy F, Haberlein I, Meier B, Frankenberger R (2007) Determination of caries risk at resin composite margins. Am J Dent 20:59–64PubMed

13.

Frankenberger R, Reinelt C, Petschelt A, Krämer N (2009) Operator vs. material influence on clinical outcome of bonded ceramic inlays. Dent Mater 25:960–968CrossRefPubMed

14.

Peumans M, Kanumilli P, De Munck J, Van Landuyt K, Lambrechts P, Van Meerbeek B (2005) Clinical effectiveness of contemporary adhesives: a systematic review of current clinical trials. Dent Mater 21:864–881CrossRefPubMed

15.

van de Sande FH, Opdam NJ, Truin GJ, Bronkhorst EM, de Soet JJ, Cenci MS, Huysmans MC (2014) The influence of different restorative materials on secondary caries development in situ. J Dent 42:1171–1177CrossRefPubMedPubMedCentral

16.

van Dijken JW, Pallesen U (2014) A randomized 10-year prospective follow-up of class II nanohybrid and conventional hybrid resin composite restorations. J Adhes Dent 16:585–592PubMed

17.

van Dijken JW, Pallesen U (2015) Randomized 3-year clinical evaluation of class I and II posterior resin restorations placed with a bulk-fill resin composite and a one-step self-etching adhesive. J Adhes Dent 17:81–88PubMed

18.

Frankenberger R, Krämer N, Petschelt A (2000) Technique sensitivity of dentin bonding: effect of application mistakes on bond strength and marginal adaptation. Oper Dent 25:324–330PubMed

19.

Hickel R, Manhart J, Garcia-Godoy F (2000) Clinical results and new developments of direct posterior restorations. Am J Dent 13:41D–54DPubMed

20.

Kidd EA, Beighton D (1996) Prediction of secondary caries around tooth-colored restorations: a clinical and microbiological study. J Dent Res 75:1942–1946CrossRefPubMed

21.

Krämer N, Möhwald M, Lücker S, Domann E, Zorzin JI, Rosentritt M, Frankenberger R (2015) Effect of microparticulate silver addition in dental adhesives on secondary caries in vitro. Clin Oral Investig 19:1673–1681CrossRefPubMed

22.

Paradella TC, Koga-Ito CY, Jorge AO (2008) Ability of different restorative materials to prevent in situ secondary caries: analysis by polarized light-microscopy and energy-dispersive X-ray. Eur J Oral Sci 116:375–380CrossRefPubMed

23.

Paradella TC, de Sousa FA, Koga-Ito CY, Jorge AO (2009) Microbiological or chemical models of enamel secondary caries compared by polarized-light microscopy and energy dispersive X-ray spectroscopy. J Biomed Mater Res B Appl Biomater 90:635–640CrossRefPubMed

24.

Seemann R, Bizhang M, Kluck I, Loth J, Roulet JF (2005) A novel in vitro microbial-based model for studying caries formation—development and initial testing. Caries Res 39:185–190CrossRefPubMed

25.

Kopperud SE, Tveit AB, Gaarden T, Sandvik L, Espelid I (2012) Longevity of posterior dental restorations and reasons for failure. Eur J Oral Sci 120:539–548CrossRefPubMed

26.

Turkun LS, Turkun M, Ertugrul F, Ates M, Brugger S (2008) Long-term antibacterial effects and physical properties of a chlorhexidine-containing glass ionomer cement. J Esthet Restor Dent 20:29–44CrossRefPubMed

27.

Benelli EM, Serra MC, Rodrigues AL Jr, Cury JA (1993) In situ anticariogenic potential of glass ionomer cement. Caries Res 27:280–284CrossRefPubMed

28.

Cenci MS, Tenuta LM, Pereira-Cenci T, Del Bel Cury AA, Ten Cate JM, Cury JA (2008) Effect of microleakage and fluoride on enamel-dentine demineralization around restorations. Caries Res 42:369–379CrossRefPubMed

29.

de Moraes MD, de Melo MA, Bezerra DD, Costa LS, Saboia VP, Rodrigues LK (2015) Clinical study of the caries-preventive effect of resin-modified glass ionomer restorations: aging versus the influence of fluoride dentifrice. J Investig Clin Dent, EPub ahead of print

30.

Dionysopoulos P, Kotsanos N, Koliniotou K, Papagodiannis Y (1994) Secondary caries formation in vitro around fluoride-releasing restorations. Oper Dent 19:183–188PubMed

31.

Dionysopoulos P, Kotsanos N, Koliniotou-Koubia E, Tolidis K (2003) Inhibition of demineralization in vitro around fluoride releasing materials. J Oral Rehabil 30:1216–1222CrossRefPubMed

32.

Hara AT, Turssi CP, Ando M, Gonzalez-Cabezas C, Zero DT, Rodrigues AL Jr, Serra MC, Cury JA (2006) Influence of fluoride-releasing restorative material on root dentine secondary caries in situ. Caries Res 40:435–439CrossRefPubMed

33.

Forsten L (1993) Clinical experience with glass ionomer for proximal fillings. Acta Odontol Scand 51:195–200CrossRefPubMed

34.

Diercke K, Lussi A, Kersten T, Seemann R (2009) Isolated development of inner (wall) caries like lesions in a bacterial-based in vitro model. Clin Oral Investig 13:439–444CrossRefPubMed

35.

Hayati F, Okada A, Kitasako Y, Tagami J, Matin K (2011) An artificial biofilm induced secondary caries model for in vitro studies. Aust Dent J 56:40–47CrossRefPubMed

36.

Sissons CH, Cutress TW (1987) In-vitro urea-dependent pH-changes by human salivary bacteria and dispersed, artificial-mouth, bacterial plaques. Arch Oral Biol 32:181–189CrossRefPubMed

37.

Sissons CH, Cutress TW (1988) pH changes during simultaneous metabolism of urea and carbohydrate by human salivary bacteria in vitro. Arch Oral Biol 33:579–587CrossRefPubMed

38.

Gilmour AS, Edmunds DH, Newcombe RG, Clark MF (1993) An in vitro study into the effect of a bacterial artificial caries system on the enamel adjacent to composite and amalgam restorations. Caries Res 27:169–175CrossRefPubMed

39.

Yu H, Wegehaupt FJ, Wiegand A, Roos M, Attin T, Buchalla W (2009) Erosion and abrasion of tooth-colored restorative materials and human enamel. J Dent 37:913–922CrossRefPubMed

40.

Swift EJ Jr, Bader JD, Shugars DA (1996) Glass-ionomer cement restorations and secondary caries. Quintessence Int 27:581–582PubMed

41.

Turssi CP, Hara AT, Domiciano SJ, Serra MC (2008) Study on the potential inhibition of root dentine wear adjacent to fluoride-containing restorations. J Mater Sci Mater Med 19:47–51CrossRefPubMed

42.

Yengopal V, Mickenautsch S (2011) Caries-preventive effect of resin-modified glass-ionomer cement (RM-GIC) versus composite resin: a quantitative systematic review. Eur Arch Paediatr Dent 12:5–14CrossRefPubMed

43.

Pendrys DG (2011) Resin-modified glass-ionomer cement (RM-GIC) may provide greater caries preventive effect compared with composite resin, but high-quality studies are needed. J Evid Based Dent Pract 11:180–182CrossRefPubMed

44.

Pereira PN, Inokoshi S, Tagami J (1998) In vitro secondary caries inhibition around fluoride releasing materials. J Dent 26:505–510CrossRefPubMed

45.

Rao A, Rao A, Sudha P (2011) Fluoride rechargability of a non-resin auto-cured glass ionomer cement from a fluoridated dentifrice: an in vitro study. J Indian Soc Pedod Prev Dent 29:202–204CrossRefPubMed

46.

Lennon AM, Wiegand A, Buchalla W, Attin T (2007) Approximal caries development in surfaces in contact with fluoride-releasing and non-fluoride-releasing restorative materials: an in situ study. Eur J Oral Sci 115:497–501CrossRefPubMed

47.

Frankenberger R, Garcia-Godoy F, Kramer N (2009) Clinical performance of viscous glass ionomer cement in posterior cavities over two years. Int J Dent 2009:781462CrossRefPubMed

48.

Mjör IA (1996) Glass-ionomer cement restorations and secondary caries: a preliminary report. Quintessence Int 27:171–174PubMed

Titel: Glass ionomer cement inhibits secondary caries in an in vitro biofilm model
verfasst von: Norbert Krämer
Miriam Schmidt
Susanne Lücker
Eugen Domann
Roland Frankenberger
Publikationsdatum: 24.07.2017
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 2/2018
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2184-1

Neu im Fachgebiet Zahnmedizin

Parodontalbehandlung verbessert Prognose bei Katheterablation

19.04.2024 Vorhofflimmern Nachrichten

Werden Personen mit Vorhofflimmern in der Blanking-Periode nach einer Katheterablation gegen eine bestehende Parodontitis behandelt, verbessert dies die Erfolgsaussichten. Dafür sprechen die Resultate einer prospektiven Untersuchung.

Invasive Zahnbehandlung: Wann eine Antibiotikaprophylaxe vor infektiöser Endokarditis schützt

11.04.2024 Endokarditis Nachrichten

Bei welchen Personen eine Antibiotikaprophylaxe zur Prävention einer infektiösen Endokarditis nach invasiven zahnärztlichen Eingriffen sinnvoll ist, wird diskutiert. Neue Daten stehen im Einklang mit den europäischen Leitlinienempfehlungen.

Zell-Organisatoren unter Druck: Mechanismen des embryonalen Zahnwachstums aufgedeckt

08.04.2024 Zahnmedizin Nachrichten

Der Aufbau von Geweben und Organen während der Embryonalentwicklung wird von den Zellen bemerkenswert choreografiert. Für diesen Prozess braucht es spezielle sogenannte „Organisatoren“. In einer aktuellen Veröffentlichung im Fachjournal Nature Cell Biology berichten Forschende durch welchen Vorgang diese Organisatoren im Gewebe entstehen und wie sie dann die Bildung von Zähnen orchestrieren.

Die Oralprophylaxe & Kinderzahnheilkunde umbenannt

11.03.2024 Kinderzahnmedizin Nachrichten

Infolge der Umbenennung der Deutschen Gesellschaft für Kinderzahnheilkunde in Deutsche Gesellschaft für Kinderzahnmedizin (DGKiZ) wird deren Mitgliederzeitschrift Oralprophylaxe & Kinderzahnheilkunde in Oralprophylaxe & Kinderzahnmedizin umbenannt. Aus diesem Grunde trägt die erste Ausgabe in 2024 erstmalig den neuen Titel.

Bestellen Sie unseren kostenlosen Newsletter Update Zahnmedizin und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Springer Medizin

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2018

Healing of root and surrounding periodontium after root damage with miniscrew implants: a histomorphologic study in dogs

Does professional experience save teeth? A survey among prosthodontists

Salivary biomarkers for oral cancer and pre-cancer screening: a review

Cleft palate with/without cleft lip in French children: radiographic evaluation of prevalence, location and coexistence of dental anomalies inside and outside cleft region

Comparison of sodium hypochlorite extrusion by five irrigation systems using an artificial root socket model and a quantitative chemical method

The effects of ozone application on genotoxic damage and wound healing in bisphosphonate-applied human gingival fibroblast cells

Neu im Fachgebiet Zahnmedizin

Parodontalbehandlung verbessert Prognose bei Katheterablation

Invasive Zahnbehandlung: Wann eine Antibiotikaprophylaxe vor infektiöser Endokarditis schützt

Zell-Organisatoren unter Druck: Mechanismen des embryonalen Zahnwachstums aufgedeckt

Die Oralprophylaxe & Kinderzahnheilkunde umbenannt

Newsletter