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01.05.2014 | Original Article

Hepatitis B virus and hepatitis D virus in blood donors from Argentina: circulation of HBsAg and reverse transcriptase mutants

verfasst von: Cecilia María Delfino, Emiliano Alberto Gentile, Amalia Inés Castillo, María Luján Cuestas, Gabriela Pataccini, Camila Cánepa, Richard Malan, Jorgelina Blejer, Carolina Berini, María Emilia Eirin, Williams Pedrozo, José Raúl Oubiña, Mirna Marcela Biglione, Verónica Lidia Mathet

Erschienen in: Archives of Virology | Ausgabe 5/2014

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Abstract

In Argentina, current procedures to ensure the safety of the blood supply for transfusion include the serologic detection of specific blood-borne infections. The aim of this study was to evaluate the prevalence and the genetic diversity of hepatitis B virus (HBV) and hepatitis D virus (HDV) in blood donor populations from two distantly located Argentine regions. Data from 56,983 blood donations from the Favaloro Foundation, in the city of Buenos Aires (Central Region), and the Central Blood Bank of Misiones Province (Northeast Region) were analyzed. Samples that were reactive for HBsAg were analyzed for HBV-DNA characterization and HDV serological and molecular analysis. The HBV prevalence was 0.12 % for HBsAg and 1.68 % for anti-HBc antibodies in Buenos Aires, and 0.73 % and 8.55 %, respectively, in Misiones. Seventy-seven HBsAg-reactive samples were analyzed by polymerase chain reaction for HBV-DNA. Subgenotypes A2, B2, C2, F1b and F4 (Buenos Aires) and F1b and D3 (Misiones) were detected. Several mutations within the major hydrophilic region of HBsAg, the reverse transcriptase, the basal core promoter, and the precore/core were detected. HDV genotype 1 was identified in Buenos Aires. This study confirms the circulation of several HBV subgenotypes, as well as known and newly identified variants, and the presence of HDV1 in this population. A thorough investigation has to be carried out to evaluate the clinical importance of some of the documented mutations as well as those detected in the HDV1 case.

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Titel: Hepatitis B virus and hepatitis D virus in blood donors from Argentina: circulation of HBsAg and reverse transcriptase mutants
verfasst von: Cecilia María Delfino
Emiliano Alberto Gentile
Amalia Inés Castillo
María Luján Cuestas
Gabriela Pataccini
Camila Cánepa
Richard Malan
Jorgelina Blejer
Carolina Berini
María Emilia Eirin
Williams Pedrozo
José Raúl Oubiña
Mirna Marcela Biglione
Verónica Lidia Mathet
Publikationsdatum: 01.05.2014
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 5/2014
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-013-1917-y

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