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01.02.2014 | Research Article

High cofilin-1 levels correlate with cisplatin resistance in lung adenocarcinomas

verfasst von: Matheus Becker, Marco Antônio De Bastiani, Carolina Beatriz Müller, Melissa M. Markoski, Mauro Antônio A. Castro, Fábio Klamt

Erschienen in: Tumor Biology | Ausgabe 2/2014

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Abstract

High cofilin-1 levels have been shown to be an accurate prognostic biomarker in non-small cell lung cancer (NSCLC) and a predictive factor in drug resistance. Herein we explore the role of cofilin-1 in cis-diamminedichloroplatinum(II) (cisplatin) resistance. We evaluated cofilin-1 levels in intrinsically cisplatin-resistant A549 (ICR-A549) cells and determined the cisplatin toxicity in A549 cells transiently transfected and overexpressing CFL1 plasmid. Moreover, expression levels (activity) of the CFL1 gene network were analyzed in a cisplatin-resistant human lung adenocarcinoma cell panel. ICR-A549 cells, selected by challenging parental cells with 10-fold drug GI₅₀ value, presented a sixfold increase in cisplatin GI₅₀ value and an increased cofilin-1 immunocontent (P < 0.01). In addition, cells transfected with cofilin-1 became more resistant to cisplatin (P < 0.01). High activity of the CFL1 gene network was found in a cisplatin-resistant adenocarcinoma cell panel (P < 0.01). In vitro evidences suggest that cofilin-1 is a biological predictor of cisplatin resistance, supporting new treatment initiatives based on cofilin-1 levels to guide chemotherapeutic interventions in NSCLC patients.

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Titel: High cofilin-1 levels correlate with cisplatin resistance in lung adenocarcinomas
verfasst von: Matheus Becker
Marco Antônio De Bastiani
Carolina Beatriz Müller
Melissa M. Markoski
Mauro Antônio A. Castro
Fábio Klamt
Publikationsdatum: 01.02.2014
Verlag: Springer Netherlands
Erschienen in: Tumor Biology / Ausgabe 2/2014
Print ISSN: 1010-4283
Elektronische ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-013-1164-6

Springer Medizin

High cofilin-1 levels correlate with cisplatin resistance in lung adenocarcinomas

Abstract

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Springer Medizin

Abstract

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