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European Spine Journal

Erschienen in:

01.06.2008 | Original Article

High glucose promotes collagen synthesis by cultured cells from rat cervical posterior longitudinal ligament via transforming growth factor-β1

verfasst von: Hai Li, Da Liu, Chang-Qing Zhao, Lei-Sheng Jiang, Li-Yang Dai

Erschienen in: European Spine Journal | Ausgabe 6/2008

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Abstract

Non-insulin-dependent diabetes is known as a risk factor of ossification of posterior longitudinal ligament, but the mechanism has not been well understood. We hypothesized that hyperglycemia, as a typical characteristic of diabetes, is closely associated with ligament hypertrophy in ossification of posterior longitudinal ligament. In this in vitro study, we investigated the effect of high glucose on collagen synthesis and transforming growth factor-β1 (TGF-β1) production using cells isolated from rat cervical posterior longitudinal ligament. The cells were subjected to high d-glucose concentration (25 mM) media for 4 days. Notable increases were observed in gene expression and protein synthesis of collagen types I, III in the cells. The increase was inhibited in the presence of anti-TGF-β1 antibodies. Production of TGF-β1 by the cells was also increased significantly by high glucose concentration. Exogenous application of TGF-β1 was confirmed to increase collagen synthesis of the cells. These data suggested that high glucose could promote collagen synthesis in the posterior longitudinal ligament mainly via endogenous TGF-β1, resulting in hypertrophy of the ligament.

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Titel: High glucose promotes collagen synthesis by cultured cells from rat cervical posterior longitudinal ligament via transforming growth factor-β1
verfasst von: Hai Li
Da Liu
Chang-Qing Zhao
Lei-Sheng Jiang
Li-Yang Dai
Publikationsdatum: 01.06.2008
Verlag: Springer-Verlag
Erschienen in: European Spine Journal / Ausgabe 6/2008
Print ISSN: 0940-6719
Elektronische ISSN: 1432-0932
DOI: https://doi.org/10.1007/s00586-008-0662-5

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High glucose promotes collagen synthesis by cultured cells from rat cervical posterior longitudinal ligament via transforming growth factor-β1

Abstract

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