Background
Methods
Patients and histological samples
Immunoblotting
Immunohistochemistry
Statistical analysis
Results
Western blot analysis
Immunohistochemical study of control sections
Immunohistochemical detection of hPXR
BBDs (n = 12) | CIS (n = 10) | IDC (n = 54) | ILC (n = 23) |
p-value1
| ||||||
---|---|---|---|---|---|---|---|---|---|---|
N | C | N | C | N | C | N | C | N | C | |
hPXR1.2
| 0 | 6 (50%) | 0 | 9 (90%) | 15 (27.8%) | 54 (100%) | 7 (30.4%) | 23 (100%) | 0.048 |
<0.001
|
hPXR1
| 0 | 4 (33.4%) | 0 | 8 (80%) | 0 | 54 (100%) | 0 | 23 (100%) | 1.000 |
<0.001
|
RXR-α | 0 | 1 (8.4%) | 0 | 2 (20%) | 23 (42.6%) | 25 (46.3%) | 11 (47.8%) | 8 (34.8%) |
0.002
|
0.037
|
RXR-β | 0 | 0 | 0 | 3 (30%) | 8 (14.8%) | 30 (55.6%) | 2 (8.7%) | 12 (52.1%) | 0.301 |
0.003
|
RXR-γ | 0 | 2 (16.7%) | 0 | 4 (40%) | 8 (14.8%) | 35 (64.8%) | 5 (21.7%) | 10 (43.5%) | 0.161 |
0.002
|
Immunohistochemical expression of retinoid receptors
Statistical analysis
hPXR1.2 C | hPXR1.2 N | hPXR1 C | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Total | Weak | High | p-value | Negative | Positive | p-value | Weak | High | p-value | ||
RXR-α C
|
Negative
| 45 | 17 | 28 |
0.035
| 24 | 21 |
<0.001
| 19 | 26 |
0.007
|
Weak
| 6 | 5 | 1 | 6 | 0 | 5 | 1 | ||||
High
| 26 | 16 | 10 | 25 | 1 | 20 | 6 | ||||
Total
| 77 | 38 | 39 | 55 | 22 | 44 | 33 | ||||
RXR-α N
|
Negative
| 43 | 30 | 13 |
<0.001
| 42 | 1 |
<0.001
| 34 | 9 |
<0.001
|
Positive
| 34 | 8 | 26 | 13 | 21 | 10 | 24 | ||||
Total
| 77 | 38 | 39 | 55 | 22 | 44 | 33 | ||||
RXR-β C
|
Negative
| 35 | 18 | 17 |
0.021
| 22 | 13 |
0.045
| 18 | 17 | 0.329 |
Weak
| 18 | 13 | 5 | 17 | 1 | 13 | 5 | ||||
High
| 24 | 7 | 17 | 16 | 8 | 13 | 11 | ||||
Total
| 77 | 38 | 39 | 55 | 22 | 44 | 33 | ||||
RXR-β N
|
Negative
| 67 | 32 | 35 | 0.470 | 48 | 19 | 0.915 | 38 | 29 | 0.845 |
Positive
| 10 | 6 | 4 | 7 | 3 | 6 | 4 | ||||
Total
| 77 | 38 | 39 | 55 | 22 | 44 | 33 | ||||
RXR-γ C
|
Negative
| 32 | 21 | 11 |
0.001
| 28 | 4 |
0.001
| 24 | 8 |
<0.001
|
Weak
| 12 | 9 | 3 | 11 | 1 | 10 | 2 | ||||
High
| 33 | 8 | 25 | 16 | 17 | 10 | 23 | ||||
Total
| 77 | 38 | 39 | 55 | 22 | 44 | 33 | ||||
RXR-γ N
|
Negative
| 64 | 34 | 30 | 0.142 | 46 | 18 | 0.847 | 37 | 27 | 0.792 |
Positive
| 13 | 4 | 9 | 9 | 4 | 7 | 6 | ||||
Total
| 77 | 38 | 39 | 55 | 22 | 44 | 33 |
hPXR1.2 C | hPXR1.2 N | hPXR1 C | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Total | Weak | High | p-value | Negative | Positive | p-value | Weak | High | p-value | ||
Tumor Type
|
Ductal
| 54 | 26 | 28 | 0.613 | 39 | 15 |
0.002
| 33 | 21 | 0.374 |
Lobular
| 22 | 12 | 10 | 16 | 6 | 11 | 11 | ||||
Age
|
<50
| 19 | 13 | 6 | 0.055 | 13 | 6 | 0.738 | 12 | 7 | 0.542 |
>50
| 58 | 25 | 33 | 42 | 16 | 32 | 26 | ||||
Tumor size
|
1
| 31 | 20 | 11 | 0.083 | 27 | 4 | 0.077 | 23 | 8 | 0.088 |
2
| 33 | 15 | 18 | 21 | 12 | 16 | 17 | ||||
3
| 5 | 1 | 4 | 3 | 2 | 2 | 3 | ||||
4
| 8 | 2 | 6 | 4 | 4 | 3 | 5 | ||||
Nodal status
|
-
| 35 | 20 | 15 | 0.212 | 30 | 5 |
0.011
| 21 | 14 | 0.644 |
+
| 42 | 18 | 24 | 25 | 17 | 23 | 19 | ||||
ER
|
0
| 17 | 7 | 10 | 0.257 | 9 | 8 |
0.001
| 10 | 7 | 0.161 |
1
| 6 | 1 | 5 | 1 | 5 | 1 | 5 | ||||
2
| 12 | 6 | 6 | 9 | 3 | 6 | 6 | ||||
3
| 42 | 24 | 18 | 36 | 6 | 27 | 15 | ||||
PR
|
0
| 21 | 10 | 11 |
0.031
| 14 | 7 |
0.000
| 12 | 9 |
0.004
|
1
| 7 | 0 | 7 | 0 | 7 | 0 | 7 | ||||
2
| 13 | 6 | 7 | 10 | 3 | 6 | 7 | ||||
3
| 36 | 22 | 14 | 31 | 5 | 26 | 10 |
RXR-α C | RXR-β C | RXR-γ C | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Negative | Weak | High | p-value | Negative | Weak | High | p-value | Negative | Weak | High | p-value | ||
Tumor Type
|
Ductal
| 30 | 3 | 21 | 0.264 | 24 | 12 | 18 | 0.838 | 19 | 10 | 25 | 0.151 |
Lobular
| 14 | 3 | 5 | 10 | 6 | 6 | 13 | 2 | 7 | ||||
Age
|
<50
| 12 | 3 | 4 | 0.185 | 10 | 4 | 5 | 0.765 | 5 | 3 | 11 | 0.254 |
>50
| 33 | 3 | 22 | 25 | 14 | 19 | 27 | 9 | 22 | ||||
Tumor size
|
1
| 15 | 4 | 12 | 0.697 | 12 | 11 | 8 | 0.292 | 15 | 7 | 9 | 0.392 |
2
| 22 | 2 | 9 | 17 | 4 | 12 | 12 | 5 | 16 | ||||
3
| 3 | 0 | 2 | 1 | 2 | 2 | 2 | 0 | 3 | ||||
4
| 5 | 0 | 3 | 5 | 1 | 2 | 3 | 0 | 5 | ||||
Nodal status
|
-
| 22 | 3 | 10 | 0.678 | 17 | 12 | 6 |
0.024
| 18 | 9 | 8 |
0.003
|
+
| 23 | 3 | 16 | 18 | 6 | 18 | 14 | 3 | 25 | ||||
ER
|
0
| 10 | 0 | 7 | 0.274 | 6 | 2 | 9 | 0.060 | 2 | 3 | 12 |
0.000
|
1
| 6 | 0 | 0 | 3 | 0 | 3 | 1 | 0 | 5 | ||||
2
| 6 | 2 | 4 | 4 | 6 | 2 | 2 | 4 | 6 | ||||
3
| 23 | 4 | 15 | 22 | 10 | 10 | 27 | 5 | 10 | ||||
PR
|
0
| 13 | 1 | 7 | 0.296 | 11 | 5 | 5 | 0.480 | 9 | 2 | 10 |
0.009
|
1
| 7 | 0 | 0 | 3 | 0 | 4 | 0 | 0 | 7 | ||||
2
| 8 | 1 | 4 | 5 | 5 | 3 | 4 | 5 | 4 | ||||
3
| 17 | 4 | 15 | 16 | 8 | 12 | 19 | 5 | 12 |
RXR-α N | RXR-β N | RXR-γ N | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Negative | Positive | p-value | Negative | Positive | p-value | Negative | Positive | p-value | ||
Tumor Type
|
Ductal
| 31 | 23 | 0.819 | 46 | 8 | 0.503 | 46 | 8 | 0.406 |
Lobular
| 12 | 10 | 20 | 2 | 17 | 5 | ||||
Age
|
<50
| 9 | 10 | 0.391 | 17 | 2 | 0.713 | 17 | 2 | 0.394 |
>50
| 34 | 24 | 50 | 8 | 47 | 11 | ||||
Tumor size
|
1
| 21 | 10 | 0.304 | 27 | 4 | 0.829 | 26 | 5 | 0.123 |
2
| 17 | 16 | 28 | 5 | 30 | 3 | ||||
3
| 2 | 3 | 5 | 0 | 3 | 2 | ||||
4
| 3 | 5 | 7 | 1 | 5 | 3 | ||||
Nodal status
|
-
| 19 | 16 | 0.802 | 28 | 7 | 0.095 | 28 | 7 | 0.505 |
+
| 24 | 18 | 39 | 3 | 36 | 6 | ||||
ER
|
0
| 8 | 9 | 0.093 | 15 | 2 | 0.254 | 16 | 1 | 0.116 |
1
| 1 | 5 | 6 | 0 | 6 | 0 | ||||
2
| 6 | 6 | 12 | 0 | 11 | 1 | ||||
3
| 28 | 14 | 34 | 8 | 31 | 11 | ||||
PR
|
0
| 13 | 8 |
0.010
| 18 | 3 | 0.608 | 17 | 4 | 0.641 |
1
| 0 | 7 | 7 | 0 | 7 | 0 | ||||
2
| 6 | 7 | 12 | 1 | 11 | 2 | ||||
3
| 24 | 12 | 30 | 6 | 29 | 7 |