nach oben

Medical Oncology

Erschienen in:

01.09.2012 | Original Paper

Immunohistochemical detection of HSP27 and hnRNP K as prognostic and predictive biomarkers for colorectal cancer

verfasst von: Feng Wang, Peng Zhang, Chenzhang Shi, Yongzhi Yang, Huanlong Qin

Erschienen in: Medical Oncology | Ausgabe 3/2012

Einloggen, um Zugang zu erhalten

Abstract

The present study was aimed at evaluating the expression of heat shock protein 27 (HSP27) and heterogeneous nuclear ribonucleoprotein K (hnRNP K), two potential biomarkers of many cancers, in colorectal cancer (CRC) and their clinical significance. Expression of HSP27 and hnRNP K were investigated by immunohistochemistry (IHC) in a series of tissue microarrays containing 175 primary colorectal cancers and their corresponding normal mucosa samples and matched with clinicopathological features and patient survival. HSP27 and hnRNP K displayed more frequent strong immunoreactivity in primary colorectal tumor samples compared with adjacent non-cancer tissue (P < 0.001). Increased cytoplasmic expression of HSP27 and hnRNP K were associated with tumor location (P = 0.032 and P < 0.001, respectively), poorer overall survival (P = 0.004 and P = 0.02, respectively) and to an unfavorable prognosis for CRC patients in multivariate analysis (P = 0.019 and P = 0.01, respectively). Their overexpression combination identified a subset of patients with definitively worse prognosis than any other combination (P < 0.001). Overexpression of HSP27 and hnRNPK were independent markers of poor prognosis, and their combination predicted definitively adverse outcomes in CRC patients.

Center MM, Jemal A, Smith RA, Ward E. Worldwide variations in colorectalcancer. CA Cancer J Clin. 2009;59:366–78.PubMedCrossRef

O’Connell JB, Maggard MA, Ko CY. Colon cancer survival rates with the new American joint committee on cancer sixth edition staging. J Natl Cancer Inst. 2004;96:1420–5.PubMedCrossRef

Celis JE, Gromov P. Proteomics in translational cancer research: toward an integrated approach. Cancer Cell. 2003;3:9–15.PubMedCrossRef

Eschrich S, Yang I, Bloom G, et al. Molecular staging for survival prediction of colorectal cancer patients. J Clin Oncol. 2005;23:3526–35.PubMedCrossRef

Kim IJ, Lim SB, Kang HC, et al. Microarray gene expression profiling for predicting complete response to preoperative chemoradiotherapy in patients with advanced rectal cancer. Dis Colon Rectum. 2007;50:1342–53.PubMedCrossRef

Battifora H, Mehta P. The checkerboard tissue block. An improved multitissue control block. Lab Invest. 1990;63:722–4.PubMed

Kononen J, Bubendorf L, Kallioniemi A, et al. Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med. 1998;4:844–7.PubMedCrossRef

Rosen DG, Huang X, Deavers MT, et al. Validation of tissue microarray technology in ovarian carcinoma. Mod Pathol. 2004;17:790–7.PubMedCrossRef

Kleiner HE, Krishnan P, Tubbs J, et al. Tissue microarray analysis of eIF4E and its downstream effector proteins in human breast cancer. J Exp Clin Cancer Res. 2009;28:5.

10.

Zhang P, Huang L, Ma YL, et al. HnRNP K and PDI marked response to chemotherapy to human colorectal cancer cells. Electrophoresis. 2010;31:1731–8.PubMedCrossRef

11.

Liu W, Ma Y, Huang L, et al. Identification of HSP27 as a potential tumor marker for colorectal cancer by the two-dimensional polyacrylamide gel electrophoresis. Mol Biol Rep. 2010;37:3207–16.PubMedCrossRef

12.

Walter SD, Feinstein AR, Wells CK. A comparison of multivariable mathematical methods for predicting survival–II. Statistical selection of prognostic variables. J Clin Epidemiol. 1990;43:349–59.PubMedCrossRef

13.

Ciocca DR, Calderwood SK. Heat shock proteins in cancer: diagnostic, prognostic, predictive, and treatment implications. Cell Stress Chaperones. 2005;10:86–103.PubMedCrossRef

14.

Kurahashi T, Miyake H, Hara I, Fujisawa M. Expression of major heat shock proteins in prostate cancer: correlation with clinicopathological outcomes in patients undergoing radical prostatectomy. J Urol. 2007;177:757–61.PubMedCrossRef

15.

Foster CS, Dodson AR, Ambroisine L, et al. Hsp-27 expression at diagnosis predicts poor clinical outcome in prostate cancer independent of ETS-gene rearrangement. Br J Cancer. 2009;101:1137–44.PubMedCrossRef

16.

Geisler JP, Tammela JE, Manahan KJ, et al. HSP27 in patients with ovarian carcinoma: still an independent prognostic indicator at 60 months follow-up. Eur J Gynaecol Oncol. 2004;25:165–8.PubMed

17.

Kapranos N, Kominea A, Konstantinopoulos PA, et al. Expression of the 27-kDa heat shock protein (HSP27) in gastric carcinomas and adjacent normal, metaplastic, and dysplastic gastric mucosa, and its prognostic significance. J Cancer Res Clin Oncol. 2002;128:426–32.PubMedCrossRef

18.

Feng JT, Liu YK, Song HY, et al. Heat-shock protein 27: a potential biomarker for hepatocellular carcinoma identified by serum proteome analysis. Proteomics. 2005;5:4581–8.PubMedCrossRef

19.

Geisler JP, Geisler HE, Tammela J, et al. A study of heat shock protein 27 in endometrial carcinoma. Gynecol Oncol. 1999;72:347–50.PubMedCrossRef

20.

Nakajima M, Kuwano H, Miyazaki T, et al. Significant correlation between expression of heat shock proteins 27, 70 and lymphocyte infiltration in esophageal squamous cell carcinoma. Cancer Lett. 2002;178:99–106.PubMedCrossRef

21.

Wang A, Liu X, Sheng S, et al. Dysregulation of heat shock protein 27 expression in oral tongue squamous cell carcinoma. BMC Cancer 2009;9:167.

22.

Pei H, Zhu H, Zeng S, et al. Proteome analysis and tissue microarray for profiling protein markers associated with lymph node metastasis in colorectal cancer. J Proteome Res. 2007;6:2495–501.PubMedCrossRef

23.

Zhao L, Liu L, Wang S, et al. Differential proteomic analysis of human colorectal carcinoma cell lines metastasis-associated proteins. J Cancer Res Clin Oncol. 2007;133:771–82.PubMedCrossRef

24.

Tweedle EM, Khattak I, Ang CW, et al. Low molecular weight heat shock protein HSP27 is a prognostic indicator in rectal cancer but not colon cancer. Gut. 2010;59:1501–10.PubMedCrossRef

25.

Frattini M, Balestra D, Suardi S, et al. Different genetic features associated with colon and rectal carcinogenesis. Clin Cancer Res. 2004;10:4015–21.PubMedCrossRef

26.

Dreyfuss G, Matunis MJ, Pinol-Roma S, Burd CG. hnRNP proteins and the biogenesis of mRNA. Annu Rev Biochem. 1993;62:289–321.PubMedCrossRef

27.

He Y, Smith R. Nuclear functions of heterogeneous nuclear ribonucleoproteins A/B. Cell Mol Life Sci. 2009;66:1239–56.PubMedCrossRef

28.

Bomsztyk K, Denisenko O, Ostrowski J. hnRNP K: one protein multiple processes. Bioessays. 2004;26:629–38.PubMedCrossRef

29.

Carpenter B, McKay M, Dundas SR, et al. Heterogeneous nuclear ribonucleoprotein K is over expressed, aberrantly localised and is associated with poor prognosis in colorectal cancer. Br J Cancer. 2006;95:921–7.PubMedCrossRef

30.

Klimek-Tomczak K, Mikula M, Dzwonek A, et al. Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa. Br J Cancer. 2006;94:586–92.PubMedCrossRef

31.

Hope NR, Murray GI. The expression profile of RNA-binding proteins in primary and metastatic colorectal cancer: relationship of heterogeneous nuclear ribonucleoproteins with prognosis. Hum Pathol. 2011;42:393–402.PubMedCrossRef

32.

Evans JR, Mitchell SA, Spriggs KA, et al. Members of the poly (rC) binding protein family stimulate the activity of the c-myc internal ribosome entry segment in vitro and in vivo. Oncogene. 2003;22:8012–20.PubMedCrossRef

33.

Lee PT, Liao PC, Chang WC, Tseng JT. Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover. Mol Biol Cell. 2007;18:5004–13.PubMedCrossRef

34.

Notari M, Neviani P, Santhanam R, et al. A MAPK/HNRPK pathway controls BCR/ABL oncogenic potential by regulating MYC mRNA translation. Blood. 2006;107:2507–16.PubMedCrossRef

35.

Inoue A, Sawata SY, Taira K, Wadhwa R. Loss-of-function screening by randomized intracellular antibodies: identification of hnRNP-K as a potential target for metastasis. Proc Natl Acad Sci USA. 2007;104:8983–8.PubMedCrossRef

36.

Neo JC, Rose P, Ong CN, Chung MC. Beta-Phenylethyl isothiocyanate mediated apoptosis: a proteomic investigation of early apoptotic protein changes. Proteomics. 2005;5:1075–82.PubMedCrossRef

37.

Rose P, Armstrong JS, Chua YL, et al. Beta-phenylethyl isothiocyanate mediated apoptosis; contribution of Bax and the mitochondrial death pathway. Int J Biochem Cell Biol. 2005;37:100–19.PubMedCrossRef

38.

Rahman-Roblick R, Roblick UJ, Hellman U, et al. p53 targets identified by protein expression profiling. Proc Natl Acad Sci USA. 2007;104:5401–6.PubMedCrossRef

Titel: Immunohistochemical detection of HSP27 and hnRNP K as prognostic and predictive biomarkers for colorectal cancer
verfasst von: Feng Wang
Peng Zhang
Chenzhang Shi
Yongzhi Yang
Huanlong Qin
Publikationsdatum: 01.09.2012
Verlag: Springer US
Erschienen in: Medical Oncology / Ausgabe 3/2012
Print ISSN: 1357-0560
Elektronische ISSN: 1559-131X
DOI: https://doi.org/10.1007/s12032-011-0037-3

Springer Medizin

Immunohistochemical detection of HSP27 and hnRNP K as prognostic and predictive biomarkers for colorectal cancer

Abstract

Neu im Fachgebiet Onkologie

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Bei Senioren mit Prostatakarzinom auf Anämie achten!

ICI-Therapie in der Schwangerschaft wird gut toleriert

Update Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2012

Prognostic factors of Chinese patients with T/NK-cell lymphoma: a single institution study of 170 patients

Clinicopathological significance and prognostic value of Xeroderma pigmentosum complementary group C (XPC) expression in sporadic breast cancer patients

An essential role of PDCD4 in progression and malignant proliferation of gastrointestinal stromal tumors

Increased serum level of Nup88 protein is associated with the development of colorectal cancer

NDRG2 down-regulation and CD24 up-regulation promote tumor aggravation and poor survival in patients with gallbladder carcinoma

Urban and non-urban disparities in the use of post-mastectomy radiation for breast cancer

Neu im Fachgebiet Onkologie

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Bei Senioren mit Prostatakarzinom auf Anämie achten!

ICI-Therapie in der Schwangerschaft wird gut toleriert

Update Onkologie