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01.01.2010 | Original Paper

Immunohistochemical study of angiogenesis after local administration of platelet-rich plasma in a patellar tendon defect

verfasst von: Dimitris Lyras, Kostas Kazakos, Dionysios Verettas, Alexandros Polychronidis, Constantinos Simopoulos, Sotirios Botaitis, George Agrogiannis, Anna Kokka, Efstratios Patsouris

Erschienen in: International Orthopaedics | Ausgabe 1/2010

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Abstract

A full thickness defect was made in the central portion of the patellar tendon of 48 New Zealand white rabbits. Platelet-rich plasma (PRP) gel was then applied and filled the tendon defect. The same procedure was performed in the control group, without the application of PRP. Animals were sacrificed after one, two, three, and four weeks. Histological and immunohistochemical analyses using a monoclonal antibody against CD31 were performed. The histological examination showed a superior healing process in the PRP group compared with the control group. Especially in the third week, the tissue formed in the PRP group was more mature and dense with less elastic fibres remaining. Neovascularisation was significantly higher in the PRP group during the first two weeks and significantly lower in the third and fourth weeks (p < 0.0001). Histological examination and study of angiogenesis showed that the application of PRP enhances and accelerates the tendon healing process.

Ahmed IM, Lagopoulos M, McConnell P, Soames RW, Sefton GK (1998) Blood supply of the Achilles tendon. J Orthop Res 16(5):591–596CrossRefPubMed

Akeda K, An HS, Pichika R, Attawia M, Thonar EJ, Lenz ME, Uchida A, Masuda K (2006) Platelet-rich plasma (PRP) stimulates the extracellular matrix metabolism of porcine nucleus pulposus and anulus fibrosus cells cultured in alginate beads. Spine 31:959–966CrossRefPubMed

Anaguchi Y, Yasuda K, Majima T, Tohyama H, Minami A, Hayashi K (2005) The effect of transforming growth factor-beta on mechanical properties of the fibrous tissue regenerated in the patellar tendon after resecting the central portion. Clin Biomech (Bristol, Avon) 20(9):959–965CrossRef

Anitua E, Sánchez M, Nurden AT, Zalduendo M, De la Fuente M, Orive G, Azofra J, Andia I (2006) Autologous fibrin matrices: a potential source of biological mediators that modulate tendon cell activities. J Biomed Mater Res A 77(2):285–293PubMed

Aspenberg P, Virchenko O (2004) Platelet concentrate injection improves Achilles tendon repair in rats. Acta Orthop Scand 75(1):93–99PubMedCrossRef

Aspenberg P (2007) Stimulation of tendon repair: mechanical loading, GDFs and platelets. A mini-review. Int Orthop 31:783–789CrossRefPubMed

Chan KM, Liu S, Maffulli N (1998) ACL reconstruction with autogenous patellar tendon graft. Donor site consideration and potential for reharvest. In: Williams and Wilkins (ed) Controversies of orthopaedic sports medicine. Asia-Pacific Ltd., Hong Kong, pp 38–45

De Mos M, van der Windt AE, Jahr H, van Schie HT, Weinans H, Verhaar JA, van Osch GJ (2008) Can platelet-rich plasma enhance tendon repair? A cell culture study. Am J Sports Med 36(6):1171–1178CrossRefPubMed

Evans CH (1999) Cytokines and the role they play in the healing of ligaments and tendons. Sports Med 28(2):71–76CrossRefPubMed

10.

Ferrara N (2004) Vascular endothelial growth factor: Basic science and clinical progress. Endocr Rev 25:581–611CrossRefPubMed

11.

Fox SB, Leek RD, Weekes MP, Whitehouse RM, Gatter KC, Harris AL (1995) Quantitation and prognostic value of breast cancer angiogenesis: comparison of microvessel density, Chalkley count, and computer image analysis. J Pathol 177:275–283CrossRefPubMed

12.

Hoffmann A, Gross G (2007) Tendon and ligament engineering in the adult organism: mesenchymal stem cells and gene-therapeutic approaches. Int Orthop 31:791–797CrossRefPubMed

13.

Ju YJ, Tohyama H, Kondo E, Yoshikawa T, Muneta T, Shinomiya K, Yasuda K (2006) Effects of local administration of vascular endothelial growth factor on properties of the in situ frozen-thawed anterior cruciate ligament in rabbits. Am J Sports Med 34(1):84–91CrossRefPubMed

14.

Kajikawa Y, Morihara T, Sakamoto H, Matsuda K, Oshima Y, Yoshida A, Nagae M, Arai Y, Kawata M, Kubo T (2008) Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing. J Cell Physiol 215(3):837–845CrossRefPubMed

15.

Kazakos K, Lyras DN, Tilkeridis K, Verettas D, Tryfonidis M (2008) The use of autologous PRP gel as an aid in the management of acute trauma wounds. Injury, Epub 12 Aug

16.

Lucarelli E, Fini M, Beccheroni A, Giavaresi G, Di Bella C, Aldini NN, Guzzardella G, Martini L, Cenacchi A, Di Maggio N, Sangiorgi L, Fornasari PM, Mercuri M, Giardino R, Donati D (2005) Stromal stem cells and platelet-rich plasma improve bone allograft integration. Clin Orthop Relat Res 435:62–68CrossRefPubMed

17.

Marumoto JM, Mitsunaga MM, Richardson AB, Medoff RJ, Mayfield GW (1996) Late patellar tendon ruptures after removal of the central third for anterior cruciate ligament reconstruction. A report of two cases. Am J Sports Med 24:698–701CrossRefPubMed

18.

Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998) Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:638–646CrossRefPubMed

19.

Miller MD, Nichols T, Butler CA (1999) Patella fracture and proximal patellar tendon rupture following arthroscopic anterior cruciate ligament reconstruction. Arthroscopy 15:640–643PubMedCrossRef

20.

Molloy T, Wang Y, Murrell G (2003) The roles of growth factors in tendon and ligament healing. Sports Med 33(5):381–394CrossRefPubMed

21.

Nagae M, Ikeda T, Mikami Y, Hase H, Ozawa H, Matsuda KI, Sakamoto H, Tabata Y, Kawata M, Kubo T (2007) Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres. Tissue Eng 13:147–158CrossRefPubMed

22.

Roldan JC, Jepsen S, Miller J, Freitag S, Rueger DC, Acil Y, Terheyden H (2004) Bone formation in the presence of platelet-rich plasma vs. bone morphogenetic protein-7. Bone 34:80–90CrossRefPubMed

23.

Sánchez M, Anitua E, Azofra J, Andía I, Padilla S, Mujika I (2007) Comparison of surgically repaired Achilles tendon tears using platelet-rich fibrin matrices. Am J Sports Med 35(20):245–251PubMed

24.

Shelbourne KD, Rubinstein RA Jr, VanMeter CD, McCarroll JR, Rettig AC (1994) Correlation of remaining patellar tendon width with quadriceps strength after autogenous bone-patellar tendon-bone anterior cruciate ligament reconstruction. Am J Sports Med 22:774–778CrossRefPubMed

25.

Shino K, Nakagawa S, Inoue M, Horibe S, Yoneda M (1993) Deterioration of patellofemoral articular surfaces after anterior cruciate ligament reconstruction. Am J Sports Med 21:206–211CrossRefPubMed

26.

Smith JJ, Ross MW, Smith RK (2006) Anabolic effects of acellular bone marrow, platelet rich plasma, and serum on equine suspensory ligament fibroblasts in vitro. Vet Comp Orthop Traumatol 19:43–47PubMed

27.

Tria AJ Jr, Alicea JA, Cody RP (1994) Patella baja in anterior cruciate ligament reconstruction of the knee. Clin Orthop 299:229–234PubMed

Titel: Immunohistochemical study of angiogenesis after local administration of platelet-rich plasma in a patellar tendon defect
verfasst von: Dimitris Lyras
Kostas Kazakos
Dionysios Verettas
Alexandros Polychronidis
Constantinos Simopoulos
Sotirios Botaitis
George Agrogiannis
Anna Kokka
Efstratios Patsouris
Publikationsdatum: 01.01.2010
Verlag: Springer-Verlag
Erschienen in: International Orthopaedics / Ausgabe 1/2010
Print ISSN: 0341-2695
Elektronische ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-009-0728-y

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