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14.11.2018 | Original Article

In vivo evaluation of the genotoxicity and oxidative damage in individuals exposed to 10% hydrogen peroxide whitening strips

verfasst von: José Francisco Del Real García, Fausto Rubén Saldaña-Velasco, Susana Vanessa Sánchez-de la Rosa, Yveth Marlene Ortiz-García, Gabriela Morales-Velazquez, Belinda Claudia Gómez-Meda, Guillermo Moisés Zúñiga-González, María Guadalupe Sánchez-Parada, Ana Lourdes Zamora-Perez

Erschienen in: Clinical Oral Investigations | Ausgabe 7/2019

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Abstract

Objective

This study assessed the impact of 10% hydrogen peroxide whitening strip exposure on the genotoxicity and oxidative damage by means of the buccal micronucleus cytome assay by counting nuclear abnormalities (NAs) in buccal mucosa and attached gingiva cells and by analyzing in whole saliva the molecule 8-hydroxy-2′-deoxyguanosine (8-OHdG).

Materials and methods

The study was conducted on 113 subjects divided into two groups: group 1 or control (n = 53), non-whitening strip exposed, and group 2 (n = 60), whitening strip exposed (Crest® 3D Whitestrips® premium plus, 10% hydrogen peroxide). Oral epithelial cells and whole saliva samples were taken at the beginning and 30 days later for group 1 and immediately before bleaching and 15 and 30 days after the end of the bleaching for group 2.

Results

An increased frequency of NAs (p < 0.05) and higher levels of 8-OHdG (p < 0.05) were observed after bleaching exposure. Also, a positive correlation exists between oxidative stress produced by hydrogen peroxide and micronuclei was found.

Conclusion

Individuals exposed to 10% hydrogen peroxide whitening strips exhibit NAs increased in oral epithelial cells and 8-OHdG in saliva, which is directed related to nuclear and oxidative DNA damage, respectively.

Clinical significance

Hydrogen peroxide is the active agent of tooth whitening and this compound induced DNA damage. Individuals exposed to whitening strips with 10% hydrogen peroxide exhibit increased genotoxic and oxidative damage. Therefore, self-application of bleaching agents should be handled carefully since it could be a risk to human health.

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Titel: In vivo evaluation of the genotoxicity and oxidative damage in individuals exposed to 10% hydrogen peroxide whitening strips
verfasst von: José Francisco Del Real García
Fausto Rubén Saldaña-Velasco
Susana Vanessa Sánchez-de la Rosa
Yveth Marlene Ortiz-García
Gabriela Morales-Velazquez
Belinda Claudia Gómez-Meda
Guillermo Moisés Zúñiga-González
María Guadalupe Sánchez-Parada
Ana Lourdes Zamora-Perez
Publikationsdatum: 14.11.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 7/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2716-3

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Abstract

Objective

Materials and methods

Results

Conclusion

Clinical significance

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