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World Journal of Urology

Erschienen in:

01.12.2008 | Original Article

Increased α1D adrenergic receptor activity and protein expression in the urinary bladder of aged rats

verfasst von: Natalia Dmitrieva, Guohua Zhang, Hiroshi Nagabukuro

Erschienen in: World Journal of Urology | Ausgabe 6/2008

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Abstract

Objectives

A possibility that aging affects (a) expression of the α1D-adrenergic receptor (AR1D), (b) AR1D-mediated contractions and (c) sympathetic innervation in the urinary bladder in rats was studied.

Materials and methods

Contraction produced by phenylephrine and inhibition of these contractions by a non-selective α1-adrenergic antagonist prazosin and a selective AR1D antagonist BMY7378 were compared between 6- and 24-month-old Fisher rats. Expressions of VMAT and AR1D in the bladder were assessed by immunofluorescence and Western blot.

Results

Phenylephrine-induced contractions were larger and inhibition of these contractions by BMY7378 was significantly greater in 24-month-old rats. Aging increased expression of AR1D in the bladder. Density of VMAT-immunoreactive neurites was decreased in smooth muscle but elevated in the suburothelial region of 24-month-old rats.

Conclusions

The results suggest that influence of adrenergic activity on bladder contractility increases with aging is due to overexpression of the AR1D. Influence of adrenergic activity on the urothelial function may also be enhanced with aging.

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Titel: Increased α1D adrenergic receptor activity and protein expression in the urinary bladder of aged rats
verfasst von: Natalia Dmitrieva
Guohua Zhang
Hiroshi Nagabukuro
Publikationsdatum: 01.12.2008
Verlag: Springer-Verlag
Erschienen in: World Journal of Urology / Ausgabe 6/2008
Print ISSN: 0724-4983
Elektronische ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-008-0292-x

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Abstract

Objectives

Materials and methods

Results

Conclusions

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