Background
Methods
Tissue specimens and antibodies
Histology and immunohistochemistry
Reverse transcription and polymerase chain reaction (RT-PCR)
Gene | Primers | Product Size | |
---|---|---|---|
Collagen types I | Forward | GTCGAGGGCCAAGACGAAG | 143 bp |
Reverse | CAGATCACGTCATCGCACAAC | ||
Collagen type III | Forward | TGGTCCCCAAGGTGTCAAAG | 117 bp |
Reverse | GGGGGTCCTGGGTTACCATTA | ||
TGF-β1 | Forward | GGCCAGATCCTGTCCAAGC | 201 bp |
Reverse | GTGGGTTTCCACCATTAGCAC | ||
TGF-β2 | Forward | TGCCATCCCGCCCACTTTC | 252 bp |
Reverse | GAGAGCCATTCGCCTTCTGC | ||
TGF-β3 | Forward | CTGTGCGTGAGTGGCTGTTG | 406 bp |
Reverse | GTGCTGTGGGTTGTGTCTGC | ||
β-actin | Forward | ATCGTGCGTGACATTAAGGAGAAG | 179 bp |
Reverse | AGGAAGGAAGGCTGGAAGAGTG |
Western blot analysis
Statistical analyses
Results
Histology analysis
Expression of collagen types I and III is up-regulated in the contraction band
Collagen Type I | Collagen Type III | TGF-β1 | TGF-β2 | TGF-β3 | |
---|---|---|---|---|---|
Adjacent Muscle | + | - | |||
Fibroblasts | + |
-
| + | ||
Endothelial cell | + |
-
| + | ||
Contraction Band | +++ | ++ | |||
Fibroblasts | +++ | + | ++ | ||
Endothelial cell | +++ | + | ++ |