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Clinical Oral Investigations

Erschienen in:

01.04.2013 | Original Article

Influence of a mouthwash containing hydroxyapatite microclusters on bacterial adherence in situ

verfasst von: C. Hannig, S. Basche, T. Burghardt, A. Al-Ahmad, M. Hannig

Erschienen in: Clinical Oral Investigations | Ausgabe 3/2013

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Abstract

Objective

The aim of the present study was to investigate the efficacy of a new preparation in dental prophylaxis containing zinc-carbonate hydroxyapatite microclusters (Biorepair) for oral biofilm management.

Methods and materials

Initial biofilm formation was carried out in situ with bovine enamel slabs fixed to individual upper jaw splints worn by six subjects. Rinses with the customary preparation as well as with subfractions (hydroxyapatite microclusters in saline solution; liquid phase without particles) were adopted for 1 min in situ after 1 min of pellicle formation, and the bacterial colonization was recorded after 6 h and 12 h, respectively. Rinses with chlorhexidine served as a reference. The adherent microorganisms were quantified and visualized using DAPI staining and live–dead staining (BacLight). Furthermore, the effects on Streptococcus mutans bacteria were tested in vitro (BacLight).

Results

Application of the customary preparation and of the separate components distinctly reduced the initial bacterial colonization of the enamel surface in situ as visualized and quantified with all techniques. After 12 h, 1.3 × 10⁷ ± 2.0 × 10⁷ bacteria/cm² were detected on unrinsed control samples with DAPI staining; 2.4 × 10⁶ ± 3.3 × 10⁶ after application of Biorepair (12 h after CHX-rinse; 1.3 × 10⁵ ± 9.2 × 10⁴). Also, pure hydroxyapatite microclusters in saline solution (2.1 × 10⁶ ± 3.0 × 10⁶) as well as the liquid phase without particles (5.1 × 10⁵ ± 3.3 × 10⁵) reduced the amount of adherent bacteria. Furthermore, antimicrobial effects on S. mutans were observed in vitro.

Conclusion

The preparation is an effective compound for biofilm management in the oral cavity due to antiadherent and antibacterial effects.

Clinical relevance

The tested mouthrinse seems to be a reasonable amendment for dental prophylaxis.

Ismail AI, Hasson H (2008) Fluoride supplements, dental caries and fluorosis: a systematic review. J Am Dent Assoc 139:1457–1468PubMed

Griffin SO, Regnier E, Griffin PM et al (2007) Effectiveness of fluoride in preventing caries in adults. J Dent Res 86:410–415PubMedCrossRef

Hellwig E, Lennon AM (2004) Systemic versus topical fluoride. Caries Res 38:258–262PubMedCrossRef

Marsh PD (2004) Dental plaque as a microbial biofilm. Caries Res 38:204–211PubMedCrossRef

Hannig C, Spies B, Spitzmuller B et al (2010) Efficacy of enzymatic mouth rinses for immobilisation of protective enzymes in the in situ pellicle. Arch Oral Biol 55:1–6PubMedCrossRef

Cross KJ, Huq NL, Reynolds EC (2007) Casein phosphopeptides in oral health—chemistry and clinical applications. Curr Pharm Des 13:793–800PubMedCrossRef

Hannig M, Hannig C (2010) Nanomaterials in preventive dentistry. Nat Nanotechnol 5:565–569PubMedCrossRef

Hannig C, Hannig M (2010) Natural enamel wear—a physiological source of hydroxylapatite nanoparticles for biofilm management and tooth repair? Med Hypotheses 74:670–672PubMedCrossRef

Roveri N, Palazzo B, Iafisco M (2008) The role of biomimetism in developing nanostructured inorganic matrices for drug delivery. Expert Opin Drug Deliv 5:861–877PubMedCrossRef

10.

Battistella E, Lelli M, Palazzo B et al. (2009) Surface enamel remineralisation: biomimetic apatite nanocrystals and fluoride ions different effects. J Nanomaterials

11.

Tschoppe P, Zandim DL, Martus P et al (2011) Enamel and dentine remineralization by nano-hydroxyapatite toothpastes. J Dent 39:430–437PubMedCrossRef

12.

Poggio C, Lombardini M, Colombo M et al (2010) Impact of two toothpastes on repairing enamel erosion produced by a soft drink: an AFM in vitro study. J Dent 38:868–874PubMedCrossRef

13.

Hannig C, Hannig M, Rehmer O et al (2007) Fluorescence microscopic visualization and quantification of initial bacterial colonization on enamel in situ. Arch Oral Biol 52:1048–1056PubMedCrossRef

14.

Hannig C, Follo M, Hellwig E et al (2010) Visualization of adherent microorganisms using different techniques. J Med Microbiol 59:1–7PubMedCrossRef

15.

Hannig C, Kirsch J, Al-Ahmad A et al. (2012) Do edible oils reduce bacterial colonization of enamel in situ? Clinical oral investigations:in press

16.

Jung DJ, Al-Ahmad A, Follo M et al (2010) Visualization of initial bacterial colonization on dentine and enamel in situ. J Microbiol Methods 81:166–174PubMedCrossRef

17.

Al-Ahmad A, Follo M, Selzer AC et al (2009) Bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization. J Med Microbiol 58:1359–1366PubMedCrossRef

18.

Schwartz T, Hoffmann S, Obst U (2003) Formation of natural biofilms during chlorine dioxide and UV disinfection in a public drinking water distribution system. J Appl Microbiol 95:591–601PubMedCrossRef

19.

Faul F, Erdfelder E, Buchner A et al (2009) Statistical power analyses using G*Power 3.1: tests for correlation and regression analyses. Behav Res Methods 41:1149–1160PubMedCrossRef

20.

Faul F, Erdfelder E, Lang AG et al (2007) G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods 39:175–191PubMedCrossRef

21.

Hannig C, Hannig M (2009) The oral cavity—a key system to understand substratum-dependent bioadhesion on solid surfaces in man. Clin Oral Investig 13:123–139PubMedCrossRef

22.

Hannig M (1999) Transmission electron microscopy of early plaque formation on dental materials in vivo. Eur J Oral Sci 107:55–64PubMedCrossRef

23.

Hannig C, Sorg J, Spitzmuller B et al (2009) Polyphenolic beverages reduce initial bacterial adherence to enamel in situ. J Dent 37:560–566PubMedCrossRef

24.

Hannig C, Spitzmuller B, Al-Ahmad A et al (2008) Effects of Cistus-tea on bacterial colonization and enzyme activities of the in situ pellicle. J Dent 36:540–545PubMedCrossRef

25.

Gjermo P, Saxton CA (1991) Antibacterial dentifrices. Clinical data and relevance with emphasis on zinc/triclosan. J Clin Periodontol 18:468–473PubMedCrossRef

26.

Soderberg TA (1990) Effects of zinc oxide, rosin and resin acids and their combinations on bacterial growth and inflammatory cells. Scand J Plast Reconstr Surg Hand Surg Suppl 22:1–87PubMed

27.

Raghupathi KR, Koodali RT, Manna AC (2011) Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir 27:4020–4028PubMedCrossRef

28.

Bowen WH (1994) Food components and caries. Adv Dent Res 8:215–220PubMed

29.

Mandel ID (1994) Antimicrobial mouthrinses: overview and update. J Am Dent Assoc 125(Suppl 2):2S–10SPubMed

30.

Sarikaya M, Tamerler C, Jen AK et al (2003) Molecular biomimetics: nanotechnology through biology. Nat Mater 2:577–585PubMedCrossRef

31.

Venegas SC, Palacios JM, Apella MC et al (2006) Calcium modulates interactions between bacteria and hydroxyapatite. J Dent Res 85:1124–1128PubMedCrossRef

32.

Kezic S, Kammeyer A, Calkoen F et al (2009) Natural moisturizing factor components in the stratum corneum as biomarkers of filaggrin genotype: evaluation of minimally invasive methods. Br J Dermatol 161:1098–1104PubMedCrossRef

33.

Rose RK (2000) Binding characteristics of Streptococcus mutans for calcium and casein phosphopeptide. Caries Res 34:427–431PubMedCrossRef

34.

Rose RK (2000) The role of calcium in oral streptococcal aggregation and the implications for biofilm formation and retention. Biochim Biophys Acta 1475:76–82PubMedCrossRef

35.

Rose RK (2000) Effects of an anticariogenic casein phosphopeptide on calcium diffusion in streptococcal model dental plaques. Arch Oral Biol 45:569–575PubMedCrossRef

36.

Boverhof DR, David RM (2010) Nanomaterial characterization: considerations and needs for hazard assessment and safety evaluation. Anal Bioanal Chem 396:953–961PubMedCrossRef

37.

Holgate ST (2010) Exposure, uptake, distribution and toxicity of nanomaterials in humans. J Biomed Nanotechnol 6:1–19PubMedCrossRef

38.

Buzea C, Pacheco I, Robbie K (2007) Nanomaterials and nanoparticles: sources and toxicity. Biointerphases 2:MR17–71PubMedCrossRef

39.

Frohlich E, Roblegg E (2012) Models for oral uptake of nanoparticles in consumer products. Toxicology 291:10–17PubMedCrossRef

Titel: Influence of a mouthwash containing hydroxyapatite microclusters on bacterial adherence in situ
verfasst von: C. Hannig
S. Basche
T. Burghardt
A. Al-Ahmad
M. Hannig
Publikationsdatum: 01.04.2013
Verlag: Springer-Verlag
Erschienen in: Clinical Oral Investigations / Ausgabe 3/2013
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-012-0781-6

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Abstract

Objective

Methods and materials

Results

Conclusion

Clinical relevance

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