nach oben

Clinical Oral Investigations

Erschienen in:

22.01.2018 | Original Article

Influence of the oscillation frequency of different side-to-side toothbrushes on noncontact biofilm removal

verfasst von: Julia C. Schmidt, Monika Astasov-Frauenhoffer, Tuomas Waltimo, Roland Weiger, Clemens Walter

Erschienen in: Clinical Oral Investigations | Ausgabe 6/2018

Einloggen, um Zugang zu erhalten

Abstract

Objectives

The objective of this study was to investigate the influence of different oscillation frequencies of three powered toothbrushes with side-to-side action for noncontact biofilm removal in an artificial interdental space model.

Materials and methods

A three-species biofilm (Porphyromonas gingivalis, Fusobacterium nucleatum and Streptococcus sanguinis) was formed in vitro on protein-coated titanium disks using a flow chamber system combined with a static biofilm growth model. The oscillation frequencies of three commercial side-to-side toothbrushes were evaluated by means of a dose response. The frequency was decreased in steps (100, 85, 70, 55, and 40%). Subsequently, the biofilm-coated substrates were exposed to the side-to-side toothbrushes. The biofilm volumes were measured using volumetric analyses (Imaris 8.1.2) with confocal laser scanning microscope images (Zeiss LSM700).

Results

Compared to maximum oscillation frequency (100%), lower oscillation frequencies (up to 40%) resulted in reduced median percentages of biofilm reduction (median biofilm reduction up to 53% for maximum oscillation frequency, and up to 13% for 40% oscillation frequency) (p ≥ 0.03). In addition, decreasing the oscillation frequencies of the side-to-side toothbrushes showed an enhanced variety in the results of repeated experiments.

Conclusions

The oscillation frequency of the tested side-to-side toothbrushes affected the biofilm reduction in an interdental space model.

Clinical relevance

Within a toothbrush, higher oscillation frequencies may lead to beneficial effects on interdental biofilm removal by noncontact brushing.

Nur mit Berechtigung zugänglich

Knöfler G, Friedl K, Fresmann S, Mausberg RF, Haak R, Ziebolz D (2017) Oral health behaviour and oral hygiene of dental professionals and laypersons—a survey performed in Lower Saxony, Germany. Oral Health Prev Dent 27:347–355

Schmidt JC, Zaugg C, Weiger R, Walter C (2013) Brushing without brushing?—a review of the efficacy of powered toothbrushes in noncontact biofilm removal. Clin Oral Investig 17(3):687–709. https://doi.org/10.1007/s00784-012-0836-8 PubMedCrossRef

Schmidt JC, Astasov-Frauenhoffer M, Hauser-Gerspach I, Schmidt JP, Waltimo T, Weiger R, Walter C (2014) Efficacy of various side-to-side toothbrushes for noncontact biofilm removal. Clin Oral Investig 18(3):793–800. https://doi.org/10.1007/s00784-013-1047-7 PubMedCrossRef

Schmidt JC, Astasov-Frauenhoffer M, Waltimo T, Weiger R, Walter C (2017) Efficacy of various side-to-side toothbrushes and impact of brushing parameters on noncontact biofilm removal in an interdental space model. Clin Oral Investig 21(5):1565–1577. https://doi.org/10.1007/s00784-016-1969-y PubMedCrossRef

Adams H, Winston MT, Heersink J, Buckingham-Meyer KA, Costerton JW, Stoodley P (2002) Development of a laboratory model to assess the removal of biofilm from interproximal spaces by powered tooth brushing. Am J Dent 15 Spec No:12B–17B

Sharma PK, Gibcus MJ, van der Mei HC, Busscher HJ (2005) Influence of fluid shear and microbubbles on bacterial detachment from a surface. Appl Environ Microbiol 71(7):3668–3673. https://doi.org/10.1128/AEM.71.7.3668-3673.2005 PubMedPubMedCentralCrossRef

Busscher HJ, Jager D, Finger G, Schaefer N, van der Mei HC (2010) Energy transfer, volumetric expansion, and removal of oral biofilms by non-contact brushing. Eur J Oral Sci 118(2):177–182. https://doi.org/10.1111/j.1600-0722.2010.00723.x PubMedCrossRef

Astasov-Frauenhoffer M, Braissant O, Hauser-Gerspach I, Weiger R, Walter C, Zitzmann NU, Waltimo T (2014) Microcalorimetric determination of the effects of amoxicillin, metronidazole, and their combination on in vitro biofilm. J Periodontol 85(2):349–357. https://doi.org/10.1902/jop.2013.120733 PubMedCrossRef

Astasov-Frauenhoffer M, Braissant O, Hauser-Gerspach I, Daniels AU, Weiger R, Waltimo T (2012) Isothermal microcalorimetry provides new insights into biofilm variability and dynamics. FEMS Microbiol Lett 337(1):31–37. https://doi.org/10.1111/1574-6968.12007 PubMedCrossRef

10.

Weiger R, Decker EM, Krastl G, Brecx M (1999) Deposition and retention of vital and dead Streptococcus sanguinis cells on glass surfaces in a flow-chamber system. Arch Oral Biol 44(8):621–628. https://doi.org/10.1016/S0003-9969(99)00061-8 PubMedCrossRef

11.

Decker EM, Maier G, Axmann D, Brecx M, Von Ohle C (2008) Effect of xylitol/chlorhexidine versus xylitol or chlorhexidine as single rinses on initial biofilm formation of cariogenic streptococci. Quintessence Int 39(1):17–22PubMed

12.

Decker EM, Weiger R, von Ohle C, Wiech I, Brecx M (2003) Susceptibility of planktonic versus attached Streptococcus sanguinis cells to chlorhexidine. Clin Oral Investig 7(2):98–102. https://doi.org/10.1007/s00784-003-0202-y PubMedCrossRef

13.

Decker EM, Weiger R, Wiech I, Heide PE, Brecx M (2003) Comparison of antiadhesive and antibacterial effects of antiseptics on Streptococcus sanguinis. Eur J Oral Sci 111(2):144–148. https://doi.org/10.1034/j.1600-0722.2003.00025.x PubMedCrossRef

14.

Hauser-Gerspach I, Kulik EM, Weiger R, Decker EM, Von Ohle C, Meyer J (2007) Adhesion of Streptococcus sanguinis to dental implant and restorative materials in vitro. Dent Mater J 26(3):361–366. https://doi.org/10.4012/dmj.26.361 PubMedCrossRef

15.

Tawakoli PN, Sauer B, Becker K, Buchalla W, Attin T (2015) Interproximal biofilm removal by intervallic use of a sonic toothbrush compared to an oral irrigation system. BMC Oral Health 15(1):91. https://doi.org/10.1186/s12903-015-0079-6 PubMedPubMedCentralCrossRef

16.

Egelberg J (1966) Permeability of the dento-gingival blood vessels. II. Clinically healthy gingivae. J Periodontal Res 1(4):276–286. https://doi.org/10.1111/j.1600-0765.1966.tb01872.x PubMedCrossRef

17.

Egelberg J (1966) Permeability of the dento-gingival blood vessels. I. Application of the vascular labelling method and gingival fluid measurements. J Periodontal Res 1(3):180–191. https://doi.org/10.1111/j.1600-0765.1966.tb01858.x PubMedCrossRef

18.

Hope CK, Petrie A, Wilson M (2003) In vitro assessment of the plaque-removing ability of hydrodynamic shear forces produced beyond the bristles by 2 electric toothbrushes. J Periodontol 74(7):1017–1022. https://doi.org/10.1902/jop.2003.74.7.1017 PubMedCrossRef

19.

Parini MR, Eggett DL, Pitt WG (2005) Removal of Streptococcus mutans biofilm by bubbles. J Clin Periodontol 32(11):1151–1156. https://doi.org/10.1111/j.1600-051X.2005.00836.x PubMedCrossRef

20.

Parini MR, Pitt WG (2005) Removal of oral biofilms by bubbles: the effect of bubble impingement angle and sonic waves. J Am Dent Assoc 136(12):1688–1693. https://doi.org/10.14219/jada.archive.2005.0112 PubMedCrossRef

21.

Parini MR, Pitt WG (2006) Dynamic removal of oral biofilms by bubbles. Colloids Surf B Biointerfaces 52(1):39–46. https://doi.org/10.1016/j.colsurfb.2006.06.005 PubMedCrossRef

22.

Pitt WG (2005) Removal of oral biofilm by sonic phenomena. Am J Dent 18(5):345–352PubMed

23.

Kolenbrander PE, Palmer RJ, Jr., Rickard AH, Jakubovics NS, Chalmers NI, Diaz PI (2006) Bacterial interactions and successions during plaque development. Periodontol 42:47–79, 1, DOI: https://doi.org/10.1111/j.1600-0757.2006.00187.x

24.

Nyvad B, Kilian M (1987) Microbiology of the early colonization of human enamel and root surfaces in vivo. Scand J Dent Res 95(5):369–380PubMed

25.

Walter C, Zahlten J, Schmeck B, Schaudinn C, Hippenstiel S, Frisch E, Hocke AC, Pischon N, Kuramitsu HK, Bernimoulin JP, Suttorp N, Krüll M (2004) Porphyromonas gingivalis strain-dependent activation of human endothelial cells. Infect Immun 72(10):5910–5918. https://doi.org/10.1128/IAI.72.10.5910-5918.2004 PubMedPubMedCentralCrossRef

26.

Walter C, Purucker P, Bernimoulin JP, Suttorp N, Meyer J, Weiger R (2005) Critical assessment of microbiological diagnostics in periodontal diseases with special focus on Porphyromonas gingivalis. Swiss Dent J 115:415–424

27.

Zahlten J, Riep B, Nichols FC, Walter C, Schmeck B, Bernimoulin JP, Hippenstiel S (2007) Porphyromonas gingivalis dihydroceramides induce apoptosis in endothelial cells. J Dent Res 86(7):635–640. https://doi.org/10.1177/154405910708600710 PubMedCrossRef

28.

Walter C, Jawor P, Bernimoulin JP, Hägewald S (2006) Moderate effect of enamel matrix derivative (Emdogain Gel) on Porphyromonas gingivalis growth in vitro. Arch Oral Biol 51(3):171–176. https://doi.org/10.1016/j.archoralbio.2005.07.005 PubMedCrossRef

29.

Jakubovics NS, Kolenbrander PE (2010) The road to ruin: the formation of disease-associated oral biofilms. Oral Dis 16(8):729–739. https://doi.org/10.1111/j.1601-0825.2010.01701.x PubMedCrossRef

30.

Zijnge V, van Leeuwen MB, Degener JE, Abbas F, Thurnheer T, Gmür R, Harmsen HJ (2010) Oral biofilm architecture on natural teeth. PLoS One 5(2):e9321. https://doi.org/10.1371/journal.pone.0009321 PubMedPubMedCentralCrossRef

31.

Lea SC, Khan A, Patanwala HS, Landini G, Walmsley AD (2007) The effects of load and toothpaste on powered toothbrush vibrations. J Dent 35(4):350–354. https://doi.org/10.1016/j.jdent.2006.11.006 PubMedCrossRef

Titel: Influence of the oscillation frequency of different side-to-side toothbrushes on noncontact biofilm removal
verfasst von: Julia C. Schmidt
Monika Astasov-Frauenhoffer
Tuomas Waltimo
Roland Weiger
Clemens Walter
Publikationsdatum: 22.01.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 6/2018
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2305-x

Neu im Fachgebiet Zahnmedizin

Parodontalbehandlung verbessert Prognose bei Katheterablation

19.04.2024 Vorhofflimmern Nachrichten

Werden Personen mit Vorhofflimmern in der Blanking-Periode nach einer Katheterablation gegen eine bestehende Parodontitis behandelt, verbessert dies die Erfolgsaussichten. Dafür sprechen die Resultate einer prospektiven Untersuchung.

Invasive Zahnbehandlung: Wann eine Antibiotikaprophylaxe vor infektiöser Endokarditis schützt

11.04.2024 Endokarditis Nachrichten

Bei welchen Personen eine Antibiotikaprophylaxe zur Prävention einer infektiösen Endokarditis nach invasiven zahnärztlichen Eingriffen sinnvoll ist, wird diskutiert. Neue Daten stehen im Einklang mit den europäischen Leitlinienempfehlungen.

Zell-Organisatoren unter Druck: Mechanismen des embryonalen Zahnwachstums aufgedeckt

08.04.2024 Zahnmedizin Nachrichten

Der Aufbau von Geweben und Organen während der Embryonalentwicklung wird von den Zellen bemerkenswert choreografiert. Für diesen Prozess braucht es spezielle sogenannte „Organisatoren“. In einer aktuellen Veröffentlichung im Fachjournal Nature Cell Biology berichten Forschende durch welchen Vorgang diese Organisatoren im Gewebe entstehen und wie sie dann die Bildung von Zähnen orchestrieren.

Die Oralprophylaxe & Kinderzahnheilkunde umbenannt

11.03.2024 Kinderzahnmedizin Nachrichten

Infolge der Umbenennung der Deutschen Gesellschaft für Kinderzahnheilkunde in Deutsche Gesellschaft für Kinderzahnmedizin (DGKiZ) wird deren Mitgliederzeitschrift Oralprophylaxe & Kinderzahnheilkunde in Oralprophylaxe & Kinderzahnmedizin umbenannt. Aus diesem Grunde trägt die erste Ausgabe in 2024 erstmalig den neuen Titel.

Bestellen Sie unseren kostenlosen Newsletter Update Zahnmedizin und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Springer Medizin

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2018

Comparison of two observational methods, scanning electron and confocal laser scanning microscopies, in the adhesive interface analysis of endodontic sealers to root dentine

The Functional Implant Prosthodontic Score (FIPS): assessment of reproducibility and observer variability

Effect of flapless ridge preservation with two different alloplastic materials in sockets with buccal dehiscence defects—volumetric and linear changes

Tranexamic acid as a local hemostasis method after dental extraction in patients on warfarin: a randomized controlled clinical study

Quantitative evaluation of dental anxiety indicators in the serum and saliva samples of children treated under general anesthesia

The effect of dental treatment on oral health-related quality of life in adolescents

Neu im Fachgebiet Zahnmedizin

Parodontalbehandlung verbessert Prognose bei Katheterablation

Invasive Zahnbehandlung: Wann eine Antibiotikaprophylaxe vor infektiöser Endokarditis schützt

Zell-Organisatoren unter Druck: Mechanismen des embryonalen Zahnwachstums aufgedeckt

Die Oralprophylaxe & Kinderzahnheilkunde umbenannt

Newsletter