Background
Methods
Chemicals and medicinal herbs
Extraction
Phytochemical screening
Ultra-performance (UPLC)-electrospray ionization (ESI)-Q/Orbitrap mass analysis
Cell culture and virus
Tissue culture infective dose (TCID50) determination
Cytotoxicity assay
Hepatotoxicity and hemolysis
Statistical analysis
Results
Phytochemical screening and tandem mass analysis
Plants | Extract Solvents | Total phenolic contents (mg GAE/g solid) | Total flavonoid contents (mg QE/g solid) | Total saponin contents (mg QSE/g solid) | Condensed tannin contents (mg CE/g solid) | Reducing sugar contents (mg GE/g solid) | Soluble Solid Contents (mg/mL) |
---|---|---|---|---|---|---|---|
Brassica juncea
| Ethanol | 1.4 ± 0.1 | 62.6 ± 3.4 | 23.5 ± 1.4 | 1.4 ± 0.1 | 0.7 ± 0.0 | 14.1 ± 0.4 |
Water | 17.9 ± 0.1 | 4.1 ± 0.0 | 62.7 ± 0.3 | 0.4 ± 0.0 | 3.5 ± 0.2 | 0.3 ± 0.2 | |
Forsythia suspensa
| Ethanol | 147.4 ± 5.8 | 242.3 ± 3.5 | 439.3 ± 4.9 | 14.1 ± 0.7 | 8.7 ± 0.9 | 25.8 ± 0.3 |
Water | 113.3 ± 4.3 | 40.7 ± 0.2 | 137.1 ± 2.2 | 0.7 ± 0.0 | 7.6 ± 0.2 | 42.4 ± 0.3 | |
Inula britannica
| Ethanol | 42.1 ± 3.3 | 225.7 ± 5.1 | 288.1 ± 7.8 | 10.2 ± 0.3 | 4.5 ± 1.6 | 18.0 ± 0.7 |
Water | 50.8 ± 2.5 | 51.6 ± 1.0 | 82.9 ± 2.6 | 0.8 ± 0.0 | 5.0 ± 0.2 | 12.8 ± 0.3 |
Medicinal herbs | Extract Solvents | Compounds | Retention time (min) | m/z [M-H] | MS2 fragment | Molecular formula (Neutral form) | Contents (μg/mg) | References |
---|---|---|---|---|---|---|---|---|
Brassica juncea
| Ethanol | Sinigrin | 1.14 | 358.02533 | 96.95830 | C10H17NO9S2 | 1.458 ± 0.027 | |
Chlorogenic acid | 18.03 | 353.20108 | 96.95840 | C16H18O9 | < LOQa | [35] | ||
Water | Sinigrin | 1.18 | 358.02857 | 96.95876 | C10H17NO9S2 | 4.116 ± 0.129 | ||
р-Coumaric acid | 5.67 | 163.03907 | 119.04838 | C9H8O3 | < LOQ | [35] | ||
Kaempferol | 13.43 | 285.04058 | C15H10O6 | < LOQ | ||||
Chlorogenic acid | 21.86 | 353.20112 | 96.95842 | C16H18O9 | < LOQ | [35] | ||
Forsythia suspensa
| Ethanol | Caffeic acid | 1.05 | 179.03373 | 135.04332 | C9H8O4 | < LOQ | [37] |
Arctigenin | 1.08 | 371.12045 | 325.18523 | C21H24O6 | 2.270 ± 0.122 | |||
Vanillic acid | 1.21 | 167.03438 | 123.04418, 108.02051 | C8H8O4 | 0.653 ± 0.131 | |||
Protocatechuic acid | 2.18 | 153.01786 | 109.02791 | C7H6O4 | 0.426 ± 0.026 | |||
Astragalin | 3.21 | 447.09172 | 269.10268 | C21H20O11 | 0.730 ± 0.027 | [38] | ||
Forsythoside A | 3.48 | 623.19776 | 179.03370 | C29H36O15 | 168.735 ± 1.549 | |||
Chlorogenic acid | 3.71 | 353.23580 | 191.05486, 96.95844 | C16H18O9 | 0.351 ± 0.010 | |||
Rutin | 4.56 | 609.14181 | 300.02756 | C27H30O16 | < LOQ | |||
Forsythoside G | 6.26 | 769.25908 | 179.03370 | C35H46O19 | < LOQ | [39] | ||
Quercetin | 12.27 | 301.03568 | 178.99739, 151.00303 | C15H10O7 | 0.245 ± 0.054 | |||
Kaempferol | 12.56 | 285.04062 | C15H10O6 | < LOQ | ||||
Water | Forsythoside A | 6.10 | 623.19745 | 179.03355 | C29H36O15 | 78.735 ± 1.703 | ||
Chlorogenic acid | 27.76 | 353.20124 | 96.95845 | C16H18O9 | 0.530 ± 0.014 | |||
Inula britannica
| Ethanol | Protocatechuic acid | 0.03 | 153.01790 | 109.02793 | C7H6O4 | 0.428 ± 0.010 | [13] |
Caffeic acid | 0.99 | 179.03378 | 135.04408 | C9H8O4 | < LOQ | [13] | ||
Chlorogenic acid | 1.15 | 353.20122 | 191.05492, 96.95845 | C16H18O9 | 0.579 ± 0.019 | [14] | ||
Ergolide | 1.17 | 305.07113 | 96.95837 | C17H22O5 | < LOQ | |||
Syringic acid | 1.47 | 197.80735 | 151.06027 | C9H10O5 | < LOQ | [13] | ||
Isoquercetin | 2.81 | 463.08749 | 300.02766 | C21H20O12 | < LOQ | [16] | ||
Nepitrin | 4.80 | 477.10246 | 315.05086 | C22H22O12 | < LOQ | |||
Rutin | 5.83 | 609.14910 | 300.02769 | C27H30O16 | 0.664 ± 0.010 | |||
Quercetin | 5.84 | 301.03572 | 178.99742, 151.00220 | C15H10O7 | 6.142 ± 0.390 | |||
Kaempferol | 6.34 | 285.04067 | C15H10O6 | 0.221 ± 0.025 | ||||
6-Methoxyluteolin | 6.50 | 315.05071 | 300.02767 | C16H12O7 | 4.261 ± 0.137 | |||
Patuletin | 9.60 | 331.04501 | 316.02324, 285.04065 | C16H12O8 | < LOQ | |||
Ferulic acid | 10.92 | 193.04926 | 147.02829 | C10H10O4 | 0.865 ± 0.012 | [13] | ||
Hispidulin | 13.60 | 299.05650 | 284.03236 | C16H12O6 | 0.867 ± 0.083 | |||
Water | Chlorogenic acid | 0.02 | 353.20114 | 191.05487 | C16H18O9 | < LOQ | [14] | |
Caffeic acid | 0.11 | 179.03377 | 135.04334 | C9H8O4 | 2.396 ± 0.028 | [13] | ||
Ergolide | 0.95 | 305.06863 | 96.95835 | C17H22O5 | < LOQ | |||
Quercetin | 1.02 | 301.20163 | 178.99738, 151.00216 | C15H10O7 | < LOQ | |||
Vanillic acid | 1.41 | 167.03338 | 123.04357 | C8H8O4 | 0.824 ± 0.028 | [13] | ||
Protocatechuic acid | 2.03 | 153.01789 | 109.02792 | C7H6O4 | 0.177 ± 0.025 | [13] | ||
6-Methoxyluteolin | 2.50 | 315.05063 | 300.02761 | C16H12O7 | < LOQ | |||
Kaempferol | 13.36 | 285.04061 | C15H10O6 | < LOQ |
Antiviral effects
Plants | Extract Solvents | Virus titration (Log TCID50a/25 μL) | ||||
---|---|---|---|---|---|---|
Total dilution (−fold) | ||||||
Control | 100 | 50 | 10 | 1b | ||
Brassica juncea
| Ethanol | 4.8 | 4.6 | 4.1** | 3.6** | 1.5** |
Water | 4.8 | 4.9 | 4.8 | 4.9 | 4.6 | |
Forsythia suspensa
| Ethanol | 4.8 | 4.6 | 3.9** | 4.0** | 3.5** |
Water | 4.8 | 4.6 | 4.3** | 3.9** | 3.8** | |
Inula britannica
| Ethanol | 4.8 | 4.5 | 4.1** | 2.5** | 0** |
Water | 4.8 | 4.9 | 4.9 | 4.6 | 3.9** |
Materials | CC50a (μg/mL) | EC50b (μg/mL) | SIc | |
Tamiflu | 569.25 ± 13.43 | 1.72 ± 0.21 | 330.170 | |
Chlorogenic acid | 72.34 ± 2.21 | 24.77 ± 1.03 | 2.920 | |
Kaempferol | 18.63 ± 0.06 | 2.46 ± 0.21 | 7.585 | |
Plants | Extract Solvents | CC50 (mg/mL) | EC50 (mg/mL) | SI |
Brassica juncea | Ethanol | 1.91 ± 0.11 | Not effective | Not effective |
Water | 9.73 ± 1.40 | 0.71 ± 0.06 | 13.668 | |
Forsythia suspensa | Ethanol | 0.02 ± 0.00 | Not effective | Not effective |
Water | 0.10 ± 0.02 | Not effective | Not effective | |
Inula britannica | Ethanol | 0.19 ± 0.02 | Not effective | Not effective |
Water | 0.68 ± 0.11 | Not effective | Not effective |