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Lasers in Medical Science

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02.03.2019 | Original Article

Irradiation by blue light-emitting diode enhances osteogenic differentiation in gingival mesenchymal stem cells in vitro

verfasst von: Tingting Zhu, Yan Wu, Xiangyu Zhou, Yaoyao Yang, Yao Wang

Erschienen in: Lasers in Medical Science | Ausgabe 7/2019

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Abstract

The aim of this study was to investigate the effects of blue light irradiation on the process of osteogenic differentiation in stem cells. The cells used in this study were derived from human gingival mesenchymal stem cells (hGMSCs), and were treated with 0 (control group), 1, 2, 4 or 6 J/cm² blue light using blue light-emitting diodes. Cell growth was assessed by the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-Diphenyl-2H-tetrazolium bromide (MTT) cell proliferation assay and osteogenic differentiation was evaluated by monitoring alkaline phosphatase (ALP) activity, alizarin red staining and real-time PCR (RT-PCR). The results of the MTT assay indicated that blue light inhibited hGMSC proliferation, and the ALP and alizarin red results showed that blue light promoted osteogenesis. The expression levels of the osteogenic genes runt-related transcription factor2 (Runx2), collagen type I (Col1) and osteocalcin (OCN) increased significantly (P < 0.05) when cells were irradiated with 2 or 4 J/cm² of blue light. In conclusion, irradiation with blue light inhibits the proliferation of hGMSC and promotes osteogenic differentiation.

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Titel: Irradiation by blue light-emitting diode enhances osteogenic differentiation in gingival mesenchymal stem cells in vitro
verfasst von: Tingting Zhu
Yan Wu
Xiangyu Zhou
Yaoyao Yang
Yao Wang
Publikationsdatum: 02.03.2019
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 7/2019
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-019-02750-3

Springer Medizin

Abstract

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