Background
Methods
Subjects
Ethical approval and sample collection
Radiography
Extraction and digestion of proteins
Separation of digested peptides
Mass spectrometry data acquisition
Protein identification
Statistical analysis
Results
Subjects | Age | Crown length; C1 (mm) | Crown length; C2 (mm) | Difference of Crown length C1-C2 (mm) | Root length; R1 (mm) | Root length; R2) (mm) | Difference of Root length R1-R2 (mm) |
---|---|---|---|---|---|---|---|
1 | 25 | 9.37 ± 0.43 | 9.08 ± 0.20 | 0.29 | 17.85 ± 0.12 | 17.50 ± 0.02 | 0.35 |
2 | 24 | 8.14 ± 0.07 | 7.89 ± 0.06 | 0.25 | 15.15 ± 0.17 | 14.71 ± 0.12 | 0.44 |
3 | 17 | 9.18 ± 0.26 | 8.96 ± 0.20 | 0.22 | 15.90 ± 0.29 | 15.28 ± 0.10 | 0.62 |
4* | 25 | 10.75 ± 0.66 | 10.23 ± 0.41 | 0.52 | 18.17 ± 0.36 | 17.30 ± 0.57 | 0.87 |
5 | 24 | 9.60 ± 0.63 | 9.33 ± 0.70 | 0.27 | 16.59 ± 0.49 | 16.15 ± 0.56 | 0.44 |
6* | 24 | 9.65 ± 0.15 | 9.50 ± 0.23 | 0.15 | 19.71 ± 0.11 | 19.57 ± 0.35 | 0.14 |
7 | 15 | 9.33 ± 0.13 | 9.13 ± 0.04 | 0.20 | 16.08 ± 0.04 | 15.66 ± 0.02 | 0.42 |
8 | 16 | 9.36 ± 0.31 | 9.03 ± 0.53 | 0.33 | 20.43 ± 0.08 | 19.43 ± 0.18 | 1.01 |
9 | 17 | 11.74 ± 0.29 | 10.83 ± 0.97 | 0.91 | 18.58 ± 0.11 | 17.60 ± 0.87 | 0.98 |
10* | 19 | 9.93 ± 0.10 | 9.39 ± 0.76 | 0.54 | 14.54 ± 1.06 | 14.18 ± 0.84 | 0.36 |
Protein name | Gene name | Accession no (SwissProt) | Biological process | Protein class | |||||
---|---|---|---|---|---|---|---|---|---|
CP | MP | ISP | L | RS | BR | ||||
Angiotensinogen | AGT | P01019 | X | Enzyme modulator | |||||
Apolipoprotein D | APOD | P05090 | X | X | – | ||||
Beta-2-glycoprotein 1 | APOH | P02749 | – | ||||||
Heat shock 70 kDa protein 1A | HSPA1A | P0DMV8 | X | X | X | X | X | – | |
Hemoglobin subunit beta | HBB | P68871 | X | X | – | ||||
Histidine-rich glycoprotein | HRG | P04196 | X | X | X | X | Enzyme modulator | ||
Ig heavy variable 4–34 | IGHV4–34 | P06331 | X | X | X | X | X | X | – |
Ig kappa variable 3–20 | IGKV3–20 | P01619 | X | Defense or immunity protein | |||||
Ig heavy variable 3–11 | IGHV3–11 | P01762 | X | X | X | X | X | X | – |
Ig heavy constant gamma 1 | IGHG1 | P01857 | X | X | X | X | X | X | – |
Ig J chain | IGJ | P01591 | – | ||||||
Thymidine phosphorylase | TYMP | P19971 | Transferase | ||||||
Protein S100-A9 | S100A9 | P06702 | X | X | Calcium-binding protein, signaling molecule | ||||
Thymosin beta-4 | TMSB4X | P62328 | X | X | X | – | |||
Zinc-alpha-2-glycoprotein | AZGP1 | P25311 | X | X | – | ||||
Vitronectin | VTN | P04004 | X | – |
Protein name | Gene name | Accession number (SwissProt) | pI/MW (kDa) | Sequest score | Peptide matched (% sequence coverage) | Fold change; differential protein abundance (↑/↓) | Student’s t-test; p-value | ||
---|---|---|---|---|---|---|---|---|---|
T1 | T3 | T6 | |||||||
Angiotensinogen | AGT | P01019 | 6.32/53.12 | 2.21 | 2.47 | 1.03↓ | 1.04↓ | 0.98↓ | 0.03 |
Apolipoprotein D | APOD | P05090 | 5.15/21.26 | 2.11 | 5.82 | 1.20↓ | 1.13↓ | 0.99↓ | 0.05 |
Beta-2-glycoprotein 1 | APOH | P02749 | 7.97/38.27 | 171.74 | 35.36 | 1.18↓ | 1.10↓ | 1.12↓ | 0.02 |
Heat shock 70 kDa protein 1A | HSPA1A | P0DMV8 | 5.66/70.01 | 96.86 | 12.79 | 1.14↓ | 0.99↑ | 1.02↑ | 0.01 |
Hemoglobin subunit beta | HBB | P68871 | 7.28/15.99 | 429.06 | 89.80 | 1.15↓ | 1.14↓ | 1.07↓ | 0.03 |
Histidine-rich glycoprotein | HRG | P04196 | 7.5/59.54 | 12.46 | 2.67 | 1.13↓ | 1.05↓ | 1.18↓ | 0.02 |
Ig heavy variable 4–34 | IGHV4–34 | P06331 | 8.28/16.22 | 16.36 | 10.96 | 1.02↓ | 0.99↑ | 1.01↑ | 0.03 |
Ig kappa variable 3–20 | IGKV3–20 | P01619 | 8.48/11.77 | 187.22 | 31.19 | 1.11↓ | 1.08↓ | 1.01↑ | 0.03 |
Ig heavy variable 3–11 | IGHV3–11 | P01762 | 9.72/13.46 | 19.17 | 15.57 | 1.08↓ | 1.03↓ | 1.12↓ | 0.04 |
Ig heavy constant gamma 1 | IGHG1 | P01857 | 8.19/36.08 | 848.34 | 51.52 | 1.12↓ | 1.07↓ | 0.99↓ | 0.03 |
Ig J chain | IGJ | P01591 | 5.24/18.09 | 15.71 | 16.35 | 0.98↑ | 0.98↑ | 1.05↑ | 0.03 |
Thymidine phosphorylase | TYMP | P19971 | 5.53/50.37 | 14.68 | 2.87 | 1.07↓ | 0.97↑ | 0.83↓ | 0.03 |
Protein S100-A9 | S100A9 | P06702 | 6.13/13.23 | 44.48 | 24.56 | 0.97↑ | 0.95↑ | 1.07↑ | 0.02 |
Thymosin beta-4 | TMSB4X | P62328 | 5.06/5.05 | 143.02 | 54.55 | 1.02↓ | 1.00↓ | 1.04↓ | 0.05 |
Zinc-alpha-2-glycoprotein | AZGP1 | P25311 | 6.05/34.24 | 108.53 | 19.46 | 0.95↑ | 0.90↑ | 1.13↑ | 0.04 |
Vitronectin | VTN | P04004 | 5.8/54.27 | 239.34 | 23.85 | 0.99↑ | 1.00↓ | 1.064↑ | 0.04 |
Pathway id | Pathway description | Observed Gene count | False discovery rate | Nodes colour | Associated proteins |
---|---|---|---|---|---|
GO:0048518 | Positive regulation of biological process | 16 | 4.31E-05 | Red | AGT, ANXA1, APOH, FGG, GAPDH, HBB, HRG, HSPA1A, IGHV3–11, IGJ, LDHA, MMP9, S100A8, S100A9, TMSB4X, VTN |
GO:0050896 | Response to stimulus | 19 | 1.10E-05 | Dark green | AGT, ANXA1, APOD, APOH, AZGP1, FGG, GAPDH, HBB, HRG, HSPA1A, IGHV3–11, IGJ, LDHA, MMP9, S100A8, S100A9, TMSB4X, TYMP, VTN |
GO:0008152 | Metabolic process | 16 | 0.0178 | Purple | AGT, AMBP, ANXA1, APOD, APOH, AZGP1, FGG, GAPDH, HBB, HSPA1A, IGHV3–11, LDHA, MMP9, S100A8, S100A9, TYMP |
GO:0009987 | Cellular process | 20 | 0.0134 | Dark blue | AGT, AMBP, ANXA1, APOD, APOH, AZGP1, FGG, GAPDH, HBB, HRG, HSPA1A, IGHV3–11, IGJ, LDHA, MMP9, S100A8, S100A9, TMSB4X, TYMP, VTN |
GO:0002376 | Immune system process | 12 | 1.42E-05 | Light green | ANXA1, FGG, GAPDH, HBB, HRG, HSPA1A, IGHV3–11, IGJ, MMP9, S100A8, S100A9, VTN |
GO:0032879 | Regulation of localization | 13 | 4.39E-06 | Yellow | AGT, ANXA1, APOD, APOH, FGG, GAPDH, HRG, HSPA1A, MMP9, S100A8, S100A9, TMSB4X, VTN |