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01.08.2003 | Article

Pancreatic NOD beta cells express MHC class II protein and the frequency of I-A^g7 mRNA-expressing beta cells strongly increases during progression to autoimmune diabetes

verfasst von: Dr. U. Walter, T. Toepfer, K. E. J. Dittmar, K. Kretschmer, J. Lauber, S. Weiss, G. Servos, O. Lechner, W. A. Scherbaum, S. R. Bornstein, H. von Boehmer, J. Buer

Erschienen in: Diabetologia | Ausgabe 8/2003

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Abstract

Aims/hypothesis

In the NOD mouse model, attempts to show MHC class II expression by pancreatic beta cells were unsuccessful so far. We readdressed this question by analysing I-A^g7 expression in single pancreatic beta cells.

Methods

Single-cell multiplex RT PCR and single-cell immunofluorescence were used to study MHC class II expression in NOD and NOD/SCID beta cells.

Results

Pancreatic beta cells from NOD mice express the I-A^g7 protein as well as the corresponding mRNA. The frequency of MHC class II mRNA-expressing beta cells is drastically increased during the progression to overt diabetes. MHC class II protein is accumulated intracellularly, and invariant chain is co-expressed. Beta cells from 9- to 10-week-old NOD/SCID mice express MHC class II at the same low frequency as beta cells from 3-week-old NOD mice.

Conclusion/interpretation

NOD beta cells express I-A^g7 and could be a direct target of autoreactive CD4+ T cells. This MHC class II expression is triggered by infiltrating lymphocytes.

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Titel: Pancreatic NOD beta cells express MHC class II protein and the frequency of I-Ag7 mRNA-expressing beta cells strongly increases during progression to autoimmune diabetes
verfasst von: Dr. U. Walter
T. Toepfer
K. E. J. Dittmar
K. Kretschmer
J. Lauber
S. Weiss
G. Servos
O. Lechner
W. A. Scherbaum
S. R. Bornstein
H. von Boehmer
J. Buer
Publikationsdatum: 01.08.2003
Verlag: Springer-Verlag
Erschienen in: Diabetologia / Ausgabe 8/2003
Print ISSN: 0012-186X
Elektronische ISSN: 1432-0428
DOI: https://doi.org/10.1007/s00125-003-1164-y

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