Erschienen in:
01.04.2013 | Original Article
Test-retest reproducibility of dopamine D2/3 receptor binding in human brain measured by PET with [11C]MNPA and [11C]raclopride
verfasst von:
Fumitoshi Kodaka, Hiroshi Ito, Yasuyuki Kimura, Saori Fujie, Harumasa Takano, Hironobu Fujiwara, Takeshi Sasaki, Kazuhiko Nakayama, Christer Halldin, Lars Farde, Tetsuya Suhara
Erschienen in:
European Journal of Nuclear Medicine and Molecular Imaging
|
Ausgabe 4/2013
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Abstract
Purpose
Dopamine D2/3 receptors (D2/3Rs) have two affinity states for endogenous dopamine, referred to as high-affinity state (D2/3
HIGH), which has a high affinity for endogenous dopamine, and low-affinity state (D2/3
LOW). The density of D2/3
HIGH can be measured with (R)-2-11CH3O-N-n-propylnorapomorphine ([11C]MNPA), while total density of D2/3
HIGH and D2/3
LOW (D2/3Rs) can be measured with [11C]raclopride using positron emission tomography (PET). Thus, the ratio of the binding potential (BP) of [11C]MNPA to that of [11C]raclopride ([11C]MNPA/[11C]raclopride) may reflect the proportion of the density of D2/3
HIGH to that of D2/3Rs. In the caudate and putamen, [11C]MNPA/[11C]raclopride reflects the proportion of the density of D2
HIGH to that of D2Rs. To evaluate the reliability of the PET paradigm with [11C]MNPA and [11C]raclopride, we investigated the test-retest reproducibility of non-displaceable BP (BP
ND) measured with [11C]MNPA and of [11C]MNPA/[11C]raclopride in healthy humans.
Methods
Eleven healthy male volunteers underwent two sets of PET studies on separate days that each included [11C]MNPA and [11C]raclopride scans. BP
ND values in the caudate and putamen were calculated. Test-retest reproducibility of BP
ND of [11C]MNPA and [11C]MNPA/[11C]raclopride was assessed by intra-subject variability (absolute variability) and test-retest reliability (intraclass correlation coefficient: ICC).
Results
The absolute variability of [11C]MNPA BP
ND was 5.30 ± 3.96 % and 12.3 ± 7.95 % and the ICC values of [11C]MNPA BP
ND were 0.72 and 0.82 in the caudate and putamen, respectively. The absolute variability of [11C]MNPA/[11C]raclopride was 6.11 ± 3.68 % and 11.60 ± 5.70 % and the ICC values of [11C]MNPA/[11C]raclopride were 0.79 and 0.80 in the caudate and putamen, respectively.
Conclusion
In the present preliminary study, the test-retest reproducibility of BP
ND of [11C]MNPA and of [11C]MNPA/[11C]raclopride was reliable in the caudate and putamen.