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International Orthopaedics

Erschienen in:

01.01.2014 | Original Paper

Human osteoblast damage after antiseptic treatment

verfasst von: Pauline Vörös, Oliver Dobrindt, Carsten Perka, Christoph Windisch, Georg Matziolis, Eric Röhner

Erschienen in: International Orthopaedics | Ausgabe 1/2014

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Abstract

Purpose

Antiseptics are powerful medical agents used for wound treatment and decontamination and have a high potential for defeating joint infections in septic surgery. Both chlorhexidine and polyhexanide are frequently used in clinical practice and have a broad antimicrobial range, but their effect on human osteoblasts has not been sufficiently studied. Our objective was to investigate the toxic effects of polyhexanide and chlorhexidine on human osteoblasts in vitro to evaluate their clinical applicability in septic surgery.

Methods

We isolated and cultivated human osteoblasts in vitro and assayed the toxic effects of chlorhexidine 0.1 % and polyhexanide 0.04 %, concentrations commonly applied in clinical practice. Toxicity analysis was performed by visualisation of cell structure, lactate dehydrogenase (LDH) activity and evaluation of vital cells. Toxicity was evaluated by microscopic inspection of cell morphology, trypan blue staining and determination of LDH release.

Results

Damaged cell structure could be shown by microscopy. Both antiseptics promoted LDH activity after incubation with osteoblasts. The evaluation of vital osteoblasts showed a significant decrease of vital cells.

Conclusions

Both antiseptics induced significant cell death of osteoblasts at optimum exposure. We therefore recommend cautious use of polyhexanide and chlorhexidine in septic surgery to avoid severe osteoblast toxicity.

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Titel: Human osteoblast damage after antiseptic treatment
verfasst von: Pauline Vörös
Oliver Dobrindt
Carsten Perka
Christoph Windisch
Georg Matziolis
Eric Röhner
Publikationsdatum: 01.01.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: International Orthopaedics / Ausgabe 1/2014
Print ISSN: 0341-2695
Elektronische ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-013-2107-y

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