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Archives of Orthopaedic and Trauma Surgery

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01.06.2009 | Orthopaedic Surgery

Mosaicplasty associated with gene enhanced tissue engineering for the treatment of acute osteochondral defects in a goat model

verfasst von: Jun Sun, Xiao-Kui Hou, Xu Li, Ting-Ting Tang, Ru-Ming Zhang, Yong Kuang, Meng Shi

Erschienen in: Archives of Orthopaedic and Trauma Surgery | Ausgabe 6/2009

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Abstract

Objective

To compare single mosaicplasty, mosaicplasty associated with gene enhanced tissue engineering and mosaicplasty associated with the gels of non-gene transduced bone mesenchymal stem cells (BMSCs) in alginate for the treatment of acute osteochondral defects in a goat model.

Methods

The principle and methods of tissue engineering were used. BMSCs were separated and amplified in vitro, and human transforming growth factor-β1 (hTGF-β1) gene was transduced to the cells. Then, the cells were suspended in the alginate. At the same time using mosaicplasty to repair the defects on the medial femoral condyle, the dead space between the cylindrical grafts were filled with the gels of hTGF-β1 gene transduced BMSCs in alginate. Single mosaicplasty and mosaicplasty associated with the gels of non-gene transduced BMSCs in alginate were compared by the different time observation.

Results

All of the three treatments could repair the acute osteochondral defects. Mosaicplasty associated with gene enhanced tissue engineering had a better integration than single mosaicplasty, and mosaicplasty associated with the gels of non-gene transduced BMSCs in alginate.

Conclusion

Mosaicplasty associated with tissue engineering could solve the problem of the poor concrescence of the remnant defect and the integration of single mosaicplasty.

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Titel: Mosaicplasty associated with gene enhanced tissue engineering for the treatment of acute osteochondral defects in a goat model
verfasst von: Jun Sun
Xiao-Kui Hou
Xu Li
Ting-Ting Tang
Ru-Ming Zhang
Yong Kuang
Meng Shi
Publikationsdatum: 01.06.2009
Verlag: Springer-Verlag
Erschienen in: Archives of Orthopaedic and Trauma Surgery / Ausgabe 6/2009
Print ISSN: 0936-8051
Elektronische ISSN: 1434-3916
DOI: https://doi.org/10.1007/s00402-008-0761-0

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Mosaicplasty associated with gene enhanced tissue engineering for the treatment of acute osteochondral defects in a goat model

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