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01.09.2008 | Original Article

Apoptotic and inflammation markers in oral mucositis in head and neck cancer patients receiving radiotherapy: preliminary report

verfasst von: Arsinoi Xanthinaki, Ourania Nicolatou-Galitis, Pavlina Athanassiadou, Maria Gonidi, Vassilis Kouloulias, Anastasia Sotiropoulou-Lontou, George Pissakas, Konstantinos Kyprianou, John Kouvaris, Efstratios Patsouris

Erschienen in: Supportive Care in Cancer | Ausgabe 9/2008

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Abstract

Goal of work

The aim of this study was to investigate the expression of pro-apoptotic protein p53 and anti-apoptotic proteins BCl-2 and MCl-1, as well as the expression of pro-inflammatory cytokines tumor necrosis factor (TNF) and interleukin-1beta (IL-1β) in patients developing mucositis during radiotherapy for head and neck cancer.

Materials and methods

Thirty-five patients receiving radiotherapy for head/neck cancer were included in this study. Patients were examined before radiotherapy. Oral mucositis was recorded weekly during radiotherapy. Cytologic smears from the oral cavity were taken with a brush. Immunocytochemical staining was performed by the use of p53, BCl-2, MCl-1 TNF and IL-1β monoclonal antibodies.

Main results

P53 was expressed in 1 of 15 smears before the initiation of radiotherapy (6.5%) compared to 3 of 7 smears from patients with grade III mucositis (43%) during radiotherapy. BCl-2 was expressed in 15 of 15 smears before radiotherapy (100%) and in three of seven patients with grade III mucositis (43%) during radiotherapy. MCl-1 was expressed in 10 of 14 samples before radiotherapy (71.5%) and in two of seven patients with grade III (28.5%) mucositis during radiotherapy. TNF was expressed in 9 of 14 patients before radiotherapy (64%) and in six of seven patients with grade III mucositis during radiotherapy (86%). IL-1β was detected in 7 of 14 patients before radiotherapy (50%) compared to 6 of 7 patients with grade III mucositis during radiotherapy (86%).

Conclusion

Our preliminary results indicate an induction of apoptosis and inflammation in the oral mucosa in patients developing mucositis during radiotherapy for head/neck cancer.

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Titel: Apoptotic and inflammation markers in oral mucositis in head and neck cancer patients receiving radiotherapy: preliminary report
verfasst von: Arsinoi Xanthinaki
Ourania Nicolatou-Galitis
Pavlina Athanassiadou
Maria Gonidi
Vassilis Kouloulias
Anastasia Sotiropoulou-Lontou
George Pissakas
Konstantinos Kyprianou
John Kouvaris
Efstratios Patsouris
Publikationsdatum: 01.09.2008
Verlag: Springer-Verlag
Erschienen in: Supportive Care in Cancer / Ausgabe 9/2008
Print ISSN: 0941-4355
Elektronische ISSN: 1433-7339
DOI: https://doi.org/10.1007/s00520-007-0379-8

Springer Medizin

Apoptotic and inflammation markers in oral mucositis in head and neck cancer patients receiving radiotherapy: preliminary report