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European Spine Journal

Erschienen in:

01.08.2015 | Review Article

The inhibitory effect of salmon calcitonin on intervertebral disc degeneration in an ovariectomized rat model

verfasst von: Yang Luo, Liu Zhang, Wen-Ya Wang, Qi-Feng Hu, Hui-Ping Song, Ying-Ze Zhang

Erschienen in: European Spine Journal | Ausgabe 8/2015

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Abstract

Purpose

Intervertebral disc degeneration related to postmenopausal osteoporosis is an important issue in spinal disorder research. This study aimed to investigate the effects of salmon calcitonin (sCT), as an antiresorptive medication, on lumbar intervertebral disc degeneration using a rat ovariectomy (OVX) model.

Methods

Thirty 3-month-old female Sprague–Dawley rats were randomly divided into three groups: the sham-operated (Sham) group and two ovariectomized groups treated with vehicle (OVX+V) or sCT (OVX+CT; 16 IU/kg, sc) on alternate days for 6 months. Treatment began after OVX and continued for 6 months. At the end of the experiment, bone mineral density (BMD), micro-CT analysis, biomechanical testing, histology, and immunohistochemistry were performed for all groups.

Results

Salmon calcitonin significantly maintained vertebrae BMD, percent bone volume, and biomechanical strength, when compared with the OVX+V group. The changes of mucoid degeneration in the nucleus pulposus and calcification in the middle cartilage endplate were more moderate in the OVX+CT group compared with the OVX+V group, and immunohistochemistry revealed a significant increase in aggrecan and type II collagen expressions, but marked reductions in matrix metalloproteinase (MMP)-1, MMP-3, and MMP-13 expressions in the OVX+CT group.

Conclusions

Salmon calcitonin treatment was effective in delaying the process of the disc degeneration in OVX rats. The underlying mechanisms may be related to preservation of structural integrity and function of vertebrae, and affecting extracellular matrix metabolism by modulating the expressions of MMPs, aggrecan and type II collagen to protect the disc from degeneration.

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Titel: The inhibitory effect of salmon calcitonin on intervertebral disc degeneration in an ovariectomized rat model
verfasst von: Yang Luo
Liu Zhang
Wen-Ya Wang
Qi-Feng Hu
Hui-Ping Song
Ying-Ze Zhang
Publikationsdatum: 01.08.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: European Spine Journal / Ausgabe 8/2015
Print ISSN: 0940-6719
Elektronische ISSN: 1432-0932
DOI: https://doi.org/10.1007/s00586-014-3611-5

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The inhibitory effect of salmon calcitonin on intervertebral disc degeneration in an ovariectomized rat model

Abstract

Purpose

Methods

Results

Conclusions

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Springer Medizin

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Purpose

Methods

Results

Conclusions

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