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01.08.2010 | Brief Review

Characterization of conformation-specific monoclonal antibodies against rabies virus nucleoprotein

verfasst von: Yan Jiang, Yonghuang Luo, Frank Michel, Robert J. Hogan, Ying He, Zhen F. Fu

Erschienen in: Archives of Virology | Ausgabe 8/2010

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Abstract

Three anti-rabies virus (RABV) nucleoprotein (N) monoclonal antibodies (Mab) were characterized by immunofluorescence assays, western blotting, and immunohistochemistry. One of these Mabs recognized the antigen by all of the assays, while the other two recognized N only in the native form in the immunofluorescence assay. These data, together with epitope mapping studies, suggest that two anti-N Mabs recognize conformational epitopes located within the N-terminal region of the RABV N protein. The availability of Mabs specific for both linear and epitope-specific antibodies should prove valuable for rabies diagnosis as well as for RABV N protein structure–function studies.

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Titel: Characterization of conformation-specific monoclonal antibodies against rabies virus nucleoprotein
verfasst von: Yan Jiang
Yonghuang Luo
Frank Michel
Robert J. Hogan
Ying He
Zhen F. Fu
Publikationsdatum: 01.08.2010
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 8/2010
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-010-0709-x

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Characterization of conformation-specific monoclonal antibodies against rabies virus nucleoprotein

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