nach oben

Erschienen in:

15.03.2019 | Original Article

Rescue and characterization of a recombinant HY12 bovine enterovirus carrying a foreign HA epitope in the 3A nonstructural protein

verfasst von: Dan Liu, Changming Liu, Xing Liu, Xin Li, Liping Huang, Junying Hu, Yanwu Wei, Hongzhen Zhu, Qun Zhang, Xinping Wang

Erschienen in: Archives of Virology | Ausgabe 5/2019

Einloggen, um Zugang zu erhalten

Abstract

Full-length infectious cDNA clones for recombinant HY12 bovine enteroviruses designated as rHY12-3A-2-HA, rHY12-3A-3-HA, and rHY12-3A-9-HA were constructed by the insertion of an epitope from influenza virus hemagglutinin (HA) at the N-terminus of the HY12-encoded 3A protein at amino acid positions 2, 3, and 9. The recombinant HY12 viruses expressing the HA epitope were rescued and characterized using immunoperoxidase monolayer assay, western blotting, and electron microscopy. The three rescued recombinant marker viruses showed similar characteristics, such as TCID₅₀ titer, plaque size, and growth properties, to those of parental rHY12 virus. Comparative analysis of the nucleotide sequences demonstrated the three recombinant marker viruses remained stable for 15 passages with no genetic changes. The recombinant viruses remained viable in various permissive cell lines, including BHK-21, Vero, and PK15 cells, suggesting that the insertion of the HA epitope tag had no effect on virus infectivity. Mice infected with the recombinant marker viruses and the parental virus produced anti-HY12-virus antibodies, while the recombinant marker viruses also produced anti-HA-epitope-tag antibodies. Taken together, these results demonstrate that HY12 viruses containing genetic markers may be useful tools for future investigations of the mechanisms of viral pathogenesis and virus replication, as well as for vaccine development.

Barya MA, Moll T, Mattson DE (1967) Antigenic analysis of bovine enteroviruses through studies of the kinetics of neutralization. Am J Vet Res 28:1283–1294PubMed

Chu JQ, Lee YJ, Park JN, Kim SM, Lee KN, Ko YJ, Lee HS, Cho IS, Kim B, Park JH (2013) Construction of a bovine enterovirus-based vector expressing a foot-and-mouth disease virus epitope. J Virol Methods 189:101–104CrossRefPubMed

Datta U, Dasgupta A (1994) Expression and subcellular localization of poliovirus VPg-precursor protein 3AB in eukaryotic cells: evidence for glycosylation in vitro. J Virol 68:4468–4477PubMedPubMedCentral

Dunne HW, Huang CM, Lin WJ (1974) Bovine enteroviruses in the calf: an attempt at serologic, biologic, and pathologic classification. J Am Vet Med Assoc 164:290–294PubMed

Forss S, Strebel K, Beck E, Schaller H (1984) Nucleotide sequence and genome organization of foot-and-mouth disease virus. Nucl Acids Res 12:6587–6601CrossRefPubMed

Fujita K, Krishnakumar SS, Franco D, Paul AV, London E, Wimmer E (2007) Membrane topography of the hydrophobic anchor sequence of poliovirus 3A and 3AB proteins and the functional effect of 3A/3AB membrane association upon RNA replication. Biochemistry 46:5185–5199CrossRefPubMedPubMedCentral

Gai X, Zhang Q, Lu H, Yang Z, Zhu L, Li X, Wang X (2018) A neonatal murine model for evaluation of enterovirus E HY12 virus infection and pathogenicity. PLoS One 13:e0193155CrossRefPubMedPubMedCentral

Giachetti C, Hwang SS, Semler BL (1992) cis-acting lesions targeted to the hydrophobic domain of a poliovirus membrane protein involved in RNA replication. J Virol 66:6045–6057PubMedPubMedCentral

Gur S, Yapkic O, Yilmaz A (2008) Serological survey of bovine enterovirus type 1 in different mammalian species in Turkey. Zoonoses Public Hl 55:106–111CrossRef

10.

Han Q, Williams WB, Saunders KO, Seaton KE, Wiehe KJ, Vandergrift N, Von Holle TA, Trama AM, Parks RJ, Luo K, Gurley TC, Kepler TB, Marshall DJ, Montefiori DC, Sutherland LL, Alam MS, Whitesides JF, Bowman CM, Permar SR, Graham BS, Mascola JR, Seed PC, Van Rompay KKA, Tomaras GD, Moody MA, Haynes BF (2017) HIV DNA-adenovirus multiclade envelope vaccine induces gp41 Antibody immunodominance in rhesus macaques. J Virol 91(21):e00923–17CrossRefPubMedPubMedCentral

11.

Giraudo AT, Beck E, Strebel K, de Mello PA, La Torre JL, Scodeller EA, Bergmann IE (1990) Identification of a nucleotide deletion in parts of polypeptide 3A in two independent attenuated aphthovirus strains. Virology 177:780–783CrossRefPubMed

12.

Jiang H, Weng L, Zhang N, Arita M, Li R, Chen L, Toyoda T (2011) Biochemical characterization of enterovirus 71 3D RNA polymerase. Biochim Biophys Acta 1809:211–219CrossRefPubMed

13.

Knox C, Moffat K, Ali S, Ryan M, Wileman T (2005) Foot-and-mouth disease virus replication sites form next to the nucleus and close to the Golgi apparatus, but exclude marker proteins associated with host membrane compartments. J Gen Virol 86:687–696CrossRefPubMed

14.

Kunin CM, Minuse E (1958) The isolation in tissue culture, chick embryo and suckling mice of filtrable agents from healthy dairy cattle. J Immunol 80:1–11PubMed

15.

Lama J, Sanz MA, Carrasco L (1998) Genetic analysis of poliovirus protein 3A: characterization of a non-cytopathic mutant virus defective in killing Vero cells. J Gen Virol 79(Pt 8):1911–1921CrossRefPubMed

16.

Li P, Bai X, Cao Y, Han C, Lu Z, Sun P, Yin H, Liu Z (2012) Expression and stability of foreign epitopes introduced into 3A nonstructural protein of foot-and-mouth disease virus. PLoS One 7:e41486CrossRefPubMedPubMedCentral

17.

Li Y, Chang J, Wang Q, Yu L (2012) Isolation of two Chinese bovine enteroviruses and sequence analysis of their complete genomes. Arch Virol 157:2369–2375CrossRefPubMed

18.

Liu C, Ihara T, Nunoya T, Ueda S (2004) Development of an ELISA based on the baculovirus-expressed capsid protein of porcine circovirus type 2 as antigen. J Vet Med Sci 66:237–242CrossRefPubMed

19.

Liu D, Liu C, Hu J, Hang L, Li X, Wei Y, Zhu H, Zhang Q, Wang X (2018) Construction and evaluation of HA-epitope-tag introduction onto the VP1 structural protein of a novel HY12 enterovirus. Virology 525:106–116CrossRefPubMed

20.

Lu HH, Li X, Cuconati A, Wimmer E (1995) Analysis of picornavirus 2A(pro) proteins: separation of proteinase from translation and replication functions. J Virol 69:7445–7452PubMedPubMedCentral

21.

Ma X, Li P, Sun P, Bai X, Bao H, Lu Z, Fu Y, Cao Y, Li D, Chen Y, Qiao Z, Liu Z (2015) Construction and characterization of 3A-epitope-tagged foot-and-mouth disease virus. Infect Genet Evol 31:17–24CrossRefPubMed

22.

Moll T, Davis AD (1959) Isolation and characterization of cytopathogenic enteroviruses from cattle with respiratory disease. Am J Vet Res 20:27–32PubMed

23.

O’Donnell VK, Pacheco JM, Henry TM, Mason PW (2001) Subcellular distribution of the foot-and-mouth disease virus 3A protein in cells infected with viruses encoding wild-type and bovine-attenuated forms of 3A. Virology 287:151–162CrossRefPubMed

24.

Paul AV (2002) Possible unifying mechanism of picornavirus genome replication. In: Semler BL (ed) In Molecular Biology of PicornaViruses. ASM Press, Washington, DC, pp 227–246

25.

Sena-Esteves M, Gao G (2018) Production of high-titer retrovirus and lentivirus vectors. Cold Spring Harb Protoc 2018(4):pdb.prot095687CrossRef

26.

Shang B, Deng C, Ye H, Xu W, Yuan Z, Shi PY, Zhang B (2013) Development and characterization of a stable eGFP enterovirus 71 for antiviral screening. Antivir Res 97:198–205CrossRefPubMed

27.

Shingu M, Chinami M, Taguchi T, Shingu M Jr (1991) Therapeutic effects of bovine enterovirus infection on rabbits with experimentally induced adult T cell leukaemia. J Gen Virol 72(Pt 8):2031–2034CrossRefPubMed

28.

Tang WF, Yang SY, Wu BW, Jheng JR, Chen YL, Shih CH, Lin KH, Lai HC, Tang P, Horng JT (2007) Reticulon 3 binds the 2C protein of enterovirus 71 and is required for viral replication. J Biol Chem 282:5888–5898CrossRefPubMed

29.

Teterina NL, Levenson EA, Ehrenfeld E (2010) Viable polioviruses that encode 2A proteins with fluorescent protein tags. J Virol 84:1477–1488CrossRefPubMed

30.

Teterina NL, Lauber C, Jensen KS, Levenson EA, Gorbalenya AE, Ehrenfeld E (2011) Identification of tolerated insertion sites in poliovirus non-structural proteins. Virology 409:1–11CrossRefPubMed

31.

Teterina NL, Pinto Y, Weaver JD, Jensen KS, Ehrenfeld E (2011) Analysis of poliovirus protein 3A interactions with viral and cellular proteins in infected cells. J Virol 85:4284–4296CrossRefPubMedPubMedCentral

32.

Towner JS, Ho TV, Semler BL (1996) Determinants of membrane association for poliovirus protein 3AB. J Biol Chem 271:26810–26818CrossRefPubMed

33.

Towner JS, Brown DM, Nguyen JH, Semler BL (2003) Functional conservation of the hydrophobic domain of polypeptide 3AB between human rhinovirus and poliovirus. Virology 314:432–442CrossRefPubMed

34.

Tsuchiaka S, Rahpaya SS, Otomaru K, Aoki H, Kishimoto M, Naoi Y, Omatsu T, Sano K, Okazaki-Terashima S, Katayama Y, Oba M, Nagai M, Mizutani T (2017) Identification of a novel bovine enterovirus possessing highly divergent amino acid sequences in capsid protein. BMC Microbiol 17:18CrossRefPubMedPubMedCentral

35.

Wang M, He J, Lu H, Liu Y, Deng Y, Zhu L, Guo C, Tu C, Wang X (2017) A novel enterovirus species identified from severe diarrheal goats. PLoS One 12:e0174600CrossRefPubMedPubMedCentral

36.

Zhu L, Xing Z, Gai X, Li S, San Z, Wang X (2014) Identification of a novel enterovirus E isolates HY12 from cattle with severe respiratory and enteric diseases. PLoS One 9:e97730CrossRefPubMedPubMedCentral

Titel: Rescue and characterization of a recombinant HY12 bovine enterovirus carrying a foreign HA epitope in the 3A nonstructural protein
verfasst von: Dan Liu
Changming Liu
Xing Liu
Xin Li
Liping Huang
Junying Hu
Yanwu Wei
Hongzhen Zhu
Qun Zhang
Xinping Wang
Publikationsdatum: 15.03.2019
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 5/2019
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-019-04178-0

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Echinokokkose medikamentös behandeln oder operieren?

06.05.2024 DCK 2024 Kongressbericht

Die Therapie von Echinokokkosen sollte immer in spezialisierten Zentren erfolgen. Eine symptomlose Echinokokkose kann – egal ob von Hunde- oder Fuchsbandwurm ausgelöst – konservativ erfolgen. Wenn eine Op. nötig ist, kann es sinnvoll sein, vorher Zysten zu leeren und zu desinfizieren.

Umsetzung der POMGAT-Leitlinie läuft

03.05.2024 DCK 2024 Kongressbericht

Seit November 2023 gibt es evidenzbasierte Empfehlungen zum perioperativen Management bei gastrointestinalen Tumoren (POMGAT) auf S3-Niveau. Vieles wird schon entsprechend der Empfehlungen durchgeführt. Wo es im Alltag noch hapert, zeigt eine Umfrage in einem Klinikverbund.

Proximale Humerusfraktur: Auch 100-Jährige operieren?

01.05.2024 DCK 2024 Kongressbericht

Mit dem demographischen Wandel versorgt auch die Chirurgie immer mehr betagte Menschen. Von Entwicklungen wie Fast-Track können auch ältere Menschen profitieren und bei proximaler Humerusfraktur können selbst manche 100-Jährige noch sicher operiert werden.

Die „Zehn Gebote“ des Endokarditis-Managements

30.04.2024 Endokarditis Leitlinie kompakt

Worauf kommt es beim Management von Personen mit infektiöser Endokarditis an? Eine Kardiologin und ein Kardiologe fassen die zehn wichtigsten Punkte der neuen ESC-Leitlinie zusammen.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 5/2019

Epidemiological trends and virological traits of hepatitis B virus infection in pregnant women and neonates

Preparation of a monoPEGylated derivative of cyanovirin-N and its virucidal effect on acyclovir-resistant strains of herpes simplex virus type 1

Novel bird’s-foot trefoil RNA viruses provide insights into a clade of legume-associated enamoviruses and rhabdoviruses

Biological characterization and pathogenicity of a newly isolated Chinese highly virulent genotype GIIa porcine epidemic diarrhea virus strain

Characterization and complete genomic analysis of two Salmonella phages, SenALZ1 and SenASZ3, new members of the genus Cba120virus