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Clinical Oral Investigations

Erschienen in:

01.04.2013 | Original Article

Osteoblast proliferation and differentiation on a barrier membrane in combination with BMP2 and TGFβ1

verfasst von: Richard J. Miron, Nikola Saulacic, Daniel Buser, Tateyuki Iizuka, Anton Sculean

Erschienen in: Clinical Oral Investigations | Ausgabe 3/2013

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Abstract

Objectives

Bioresorbable collagen membranes are routinely utilized in guided bone regeneration to selectively direct the growth and repopulation of bone cells in areas of insufficient volume. However, the exact nature by which alveolar osteoblasts react to barrier membranes as well as the effects following the addition of growth factors to the membranes are still poorly understood. The objective of the present study was therefore to investigate the effect of a bioresorbable collagen membrane soak-loaded in growth factors bone morphogenetic protein 2 (BMP2) or transforming growth factor β1 (TGFβ1) on osteoblast adhesion, proliferation, and differentiation.

Material and methods

Prior to experimental seeding, membranes were soaked in either BMP2 or TGFβ1 at a concentration of 10 ng/ml for 5 min.

Results

Human osteoblasts adhered to all soak-loaded membranes as assessed by scanning electron microscopy. Growth factors BMP2 and TGFβ1 increased osteoblast proliferation at 3 or 5 days post-seeding when compared to control collagen membranes. Analysis of real-time PCR revealed that administration of BMP2 increased osteoblast differentiation markers such as osterix, collagen I, and osteocalcin. BMP2 also increased mineralization of primary osteoblasts as demonstrated by alizarin red staining when compared to control and TGFβ1 soak-loaded membranes.

Conclusion

The combination of a collagen barrier membrane with growth factors TGFβ1 and BMP2 significantly influenced adhesion, proliferation, and differentiation of primary human osteoblasts.

Clinical relevance

The described in vitro effects following the combination of collagen barrier membranes with growth factors TGFβ1 and BMP2 provide further biologic support for the clinical application of this treatment strategy in guided bone regeneration procedures.

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Titel: Osteoblast proliferation and differentiation on a barrier membrane in combination with BMP2 and TGFβ1
verfasst von: Richard J. Miron
Nikola Saulacic
Daniel Buser
Tateyuki Iizuka
Anton Sculean
Publikationsdatum: 01.04.2013
Verlag: Springer-Verlag
Erschienen in: Clinical Oral Investigations / Ausgabe 3/2013
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-012-0764-7

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Osteoblast proliferation and differentiation on a barrier membrane in combination with BMP2 and TGFβ1

Abstract

Objectives

Material and methods

Results

Conclusion

Clinical relevance

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Springer Medizin

Abstract

Objectives

Material and methods

Results

Conclusion

Clinical relevance

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