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Clinical Oral Investigations
Erschienen in: Clinical Oral Investigations 7/2014

01.09.2014 | Original Article

Blue light kills Aggregatibacter actinomycetemcomitans due to its endogenous photosensitizers

verfasst von: Fabian Cieplik, Andreas Späth, Christoph Leibl, Anita Gollmer, Johannes Regensburger, Laura Tabenski, Karl-Anton Hiller, Tim Maisch, Gottfried Schmalz

Erschienen in: Clinical Oral Investigations | Ausgabe 7/2014

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Abstract

Objectives

The aim of this study was to demonstrate that the periodontal pathogen Aggregatibacter actinomycetemcomitans (AA) can be killed by irradiation with blue light derived from a LED light-curing unit due to its endogenous photosensitizers.

Materials and methods

Planktonic cultures of AA and Escherichia coli were irradiated with blue light from a bluephase® C8 light-curing unit with an emission peak at 460 nm, which is usually applied for polymerization of dental resins. A CFU-assay was performed for the analysis of viable bacteria after treatment. Moreover, bacterial cells were lysed and the lysed AA and E. coli were investigated for generation of singlet oxygen. Spectroscopic measurements of lysed AA and E. coli were performed and analyzed for characteristic absorption and emission peaks.

Results

A light dose of 150 J/cm2 induced a reduction of ≥5 log10 steps of viable AA, whereas no effect of blue light was found against E. coli. Spectrally resolved measurements of singlet oxygen luminescence showed clearly that a singlet oxygen signal is generated from lysed AA upon excitation at 460 nm. Spectroscopic measurements of lysed AA exhibited characteristic absorption and emission peaks similar to those of known porphyrins and flavins.

Conclusions

AA can be inactivated by irradiation with blue light only, without application of an exogenous photosensitizer.

Clinical relevance

These results encourage further studies on the potential use of these blue light-mediated auto-photosensitization processes in the treatment of periodontitis for the successful inactivation of Aggregatibacter actinomycetemcomitans.
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Metadaten
Titel
Blue light kills Aggregatibacter actinomycetemcomitans due to its endogenous photosensitizers
verfasst von
Fabian Cieplik
Andreas Späth
Christoph Leibl
Anita Gollmer
Johannes Regensburger
Laura Tabenski
Karl-Anton Hiller
Tim Maisch
Gottfried Schmalz
Publikationsdatum
01.09.2014
Verlag
Springer Berlin Heidelberg
Erschienen in
Clinical Oral Investigations / Ausgabe 7/2014
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-013-1151-8

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