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01.07.2015 | Original Article

Hypertension modifies OPG, RANK, and RANKL expression during the dental socket bone healing process in spontaneously hypertensive rats

verfasst von: Natalia Manrique, Cassiano Costa Silva Pereira, Eloá Rodrigues Luvizuto, Maria Del Pilar Rodriguez Sánchez, Tetuo Okamoto, Roberta Okamoto, Doris H. Sumida, Cristina Antoniali

Erschienen in: Clinical Oral Investigations | Ausgabe 6/2015

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Abstract

Objective

The aim of this study was to evaluate the dental socket bone healing process by histomorphometric and immunohistochemical analysis of osteoprotegerin (OPG), receptor activator of nuclear factor-κβ (RANK), and receptor activator of nuclear factor-κβ ligand (RANKL) proteins in spontaneously hypertensive rats (SHR).

Materials and methods

Under general anesthesia, 25 Wistar rats and 25 SHRs underwent upper right incisor extraction. Rats were euthanized after 7, 14, 21, 28, or 42 days of dental extractions. Histomorphometric and immunohistochemical analyses of OPG, RANK, and RANKL proteins were performed.

Results

Histomorphometric results showed decreased bone healing and reduced bone trabecular thickness in SHRs. Immunohistochemical reactions showed intense RANKL and RANK immunolabeling at 14 and 28 postoperative days and mild OPG immunolabeling at 7, 14, and 21 days after surgery in SHRs.

Conclusion

The results of this study show that RANK, RANKL, and OPG immunolabeling was altered in SHRs, and these results are associated with bone healing delay and decreased trabecular thickness in SHRs.

Clinical relevance

Hypertension alters the expression of RANK, RANKL, and OPG and delays the socket bone healing process. These alterations could influence some dental procedures such as orthodontic treatment and implant placement.

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Titel: Hypertension modifies OPG, RANK, and RANKL expression during the dental socket bone healing process in spontaneously hypertensive rats
verfasst von: Natalia Manrique
Cassiano Costa Silva Pereira
Eloá Rodrigues Luvizuto
Maria Del Pilar Rodriguez Sánchez
Tetuo Okamoto
Roberta Okamoto
Doris H. Sumida
Cristina Antoniali
Publikationsdatum: 01.07.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 6/2015
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-014-1369-0

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Hypertension modifies OPG, RANK, and RANKL expression during the dental socket bone healing process in spontaneously hypertensive rats

Abstract

Objective

Materials and methods

Results

Conclusion

Clinical relevance

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Springer Medizin

Abstract

Objective

Materials and methods

Results

Conclusion

Clinical relevance

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