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Clinical Oral Investigations
Erschienen in: Clinical Oral Investigations 7/2016

01.12.2015 | Original Article

Regulation of p53 under hypoxic and inflammatory conditions in periodontium

verfasst von: S. Memmert, L. Gölz, P. Pütz, A. Jäger, J. Deschner, T. Appel, G. Baumgarten, B. Rath-Deschner, S. Frede, W. Götz

Erschienen in: Clinical Oral Investigations | Ausgabe 7/2016

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Abstract

Objectives

Different studies suggest that inflammation as well as hypoxia leads to an increase of p53 protein levels. However, the implication of p53 during oral inflammatory processes is still unknown. The aim of this study was therefore to investigate the effect of hypoxia and inflammation on p53 regulation in human periodontium in vitro and in vivo.

Materials and methods

Under hypoxic and normoxic conditions, human primary periodontal ligament (PDL) fibroblasts (n = 9) were stimulated with lipopolysaccharides (LPS) from Porphyromonas gingivalis (P.g.), a periodontal pathogenic bacterium. After different time points, cell viability was tested; p53 gene expression, protein synthesis, and activation were measured using quantitative RT-PCR, immunoblotting, and immunofluorescence. Moreover, healthy and inflamed periodontal tissues were obtained from 12 donors to analyze p53 protein in oral inflammatory diseases by immunohistochemistry.

Results

LPS-P.g. and hypoxia initially induced a significant upregulation of p53 mRNA expression and p53 protein levels. Nuclear translocation of p53 after inflammatory stimulation supported these findings. Hypoxia first enhanced p53 levels, but after 24 h of incubation, protein levels decreased, which was accompanied by an improvement of PDL cell viability. Immunohistochemistry revealed an elevation of p53 immunoreactivity in accordance to the progression of periodontal inflammation.

Conclusions

Our data indicate that p53 plays a pivotal role in PDL cell homeostasis and seems to be upregulated in oral inflammatory diseases.

Clinical relevance

Upregulation of p53 may promote the destruction of periodontal integrity. A possible relationship with carcinogenesis may be discussed.
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Metadaten
Titel
Regulation of p53 under hypoxic and inflammatory conditions in periodontium
verfasst von
S. Memmert
L. Gölz
P. Pütz
A. Jäger
J. Deschner
T. Appel
G. Baumgarten
B. Rath-Deschner
S. Frede
W. Götz
Publikationsdatum
01.12.2015
Verlag
Springer Berlin Heidelberg
Erschienen in
Clinical Oral Investigations / Ausgabe 7/2016
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-015-1679-x

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