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Lasers in Medical Science
Erschienen in: Lasers in Medical Science 4/2004

01.03.2004 | Original Article

Analysis of mitochondria, endoplasmic reticulum and actin filaments after PDT with AlPcS4

verfasst von: S. D. R. M. Ferreira, A. C. Tedesco, G. Sousa, R. A. Zângaro, N. S. Silva, M. T. T. Pacheco, C. Pacheco-Soares

Erschienen in: Lasers in Medical Science | Ausgabe 4/2004

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Abstract

Photodynamic therapy (PDT) is a therapeutic modality for the treatment of tumors. This technique uses a visible light to activate a photosensitizer compounds, leading to a photo-oxidation process of biological tissue that can induce apoptosis or necrosis both in vivo and in vitro. However many of the cytotoxic effects remain an open question to be investigated. The cytotoxicity to specific cellular targets of classical photosensitizers used in the PDT in vitro has been analyzed in this work. The photosensitizing effects of Chloroaluminum Phthalocyanine Tetrasulfonate (AlPcS4) were studied on the mitochondria, cytoskeleton and endoplasmic reticulum of HeLa cells. The cells were irradiated with a diode laser (working at 670 nm; energy density of 4.5 J/cm2 and power density of 45 mW/cm2). The spectrofluorimetric analysis of the mitochondria showed changes in membrane potential. Cytoskeleton and endoplasmic reticulum showed basic alterations in distribution after PDT treatment, as an indicator of cellular death process.
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Metadaten
Titel
Analysis of mitochondria, endoplasmic reticulum and actin filaments after PDT with AlPcS4
verfasst von
S. D. R. M. Ferreira
A. C. Tedesco
G. Sousa
R. A. Zângaro
N. S. Silva
M. T. T. Pacheco
C. Pacheco-Soares
Publikationsdatum
01.03.2004
Verlag
Springer-Verlag
Erschienen in
Lasers in Medical Science / Ausgabe 4/2004
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-003-0282-6

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