nach oben

Lasers in Medical Science

Erschienen in:

01.01.2008 | Original Article

Toluidine blue-mediated photoinactivation of periodontal pathogens from supragingival plaques

verfasst von: Yanli Qin, Xiuling Luan, Liangjia Bi, Guopei He, XueFeng Bai, Chuannong Zhou, Zhiguo Zhang

Erschienen in: Lasers in Medical Science | Ausgabe 1/2008

Einloggen, um Zugang zu erhalten

Abstract

This study aimed to assess the effect of toluidine blue (TB)-mediated photodynamic inactivation of periodontal pathogens (PP) from periodontopathic patients. Photodynamic therapy (PDT) was carried out using TB and 635 nm laser light irradiation. The bactericidal effect was evaluated, and important PDT parameters including light intensity, energy dose, and TB concentration were determined. Our findings suggest that TB-mediated lethal photosensitization of PP in vivo is possible. However, to obtain ideal bactericidal effect, higher doses of light and photosensitizer should be required in treatment in vivo than their planktonic counterparts. The best therapeutic effect was observed in treatment by 1 mg/ml TB combined with 12 J/cm² at 159 mW/cm² light irradiation. Moreover, because of the considerable interindividual differences of bacterial populations, TB-mediated PDT might not be equally effective among periodontopathic patients, and further studies on improvement of this therapeutic modality is needed.

Slot J (1979) Subgingival microflora and periodontal disease. J Clin Periodontol 6:351–356CrossRef

Haper OR, Dymock D, Booth V, Weightman AJ, Wade WG (1999) Detection of unculturable bacteria in periodontal health and disease by PCR. J Clin Microbiol 37:1469–1473

Killoy WJ (1998) Chemical treatment of periodontitis: local delivery of antimicrobials. Int Dent J 48:305–315PubMed

Rams TE, Slots J (1996) Local delivery of antimicrobial agents in the periodontal pocket. Periodontol 2000 10:139–159PubMedCrossRef

Wasserman B, Hirschfeld I (1998) The relationship of initial clinical parameters to the long-term response in 112 cases of periodontal disease. J Clin Periodontol 15:38–42CrossRef

Silver JG, Martin AW (1980) Experiment transient bacteraemias in human subjects with varying degrees of plaque accumulation and gingival inflammation. J Clin Periodontol 4:92–99CrossRef

Sconyers JR, Crawford JJ, Moriarty JD (1973) Relationship of bacteremia to toothbrushing in patients with periodontitis. J Am Dent Assoc 87:616–692PubMed

Walker CB (1996) The acquisition of antibiotic resistance in the periodontal microflora. Periodontol 2000 10:79–88PubMedCrossRef

Dougherty TJ, Gomer CJ, Henderson BW et al (1998) Photodynamic therapy. J Natl Cancer Inst 90:889–905PubMedCrossRef

10.

Dougherty TJ (2002) An update on photodynamic therapy applications. J Clin Laser Med Surg 20:3–7PubMedCrossRef

11.

Berthiaume F, Reiken SR, Toner M, Tompkins RG, Yarmush ML (1994) Antibody-targeted photolysis of bacteria in vivo. Biotechnology 12:703–705PubMedCrossRef

12.

Bhatti M, MacRobert A, Meghji S, Henderson B, Wilson M (1997) Effect of dosimetric and physiological factors on the lethal photosensitization of Porphyromonas gingivalis in vitro. Photochem Photobiol 65:1026–1031PubMed

13.

Bhatti M, MacRobert A, Meghji S, Henderson B, Wilson M (1998) A study of the uptake of toluidine blue O by Porphyromonas gingivalis and the mechanism of lethal photosensitisation. Photochem Photobiol 68:370–376PubMedCrossRef

14.

Chaves ES, Jeffcoat MK, Ryerson CC, Snyder B (2000) Persistent bacterial colonization of Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans in periodontitis and its association with alveolar bone loss after 6 months of therapy. J Clin Periodontol 27:897–903PubMedCrossRef

15.

Ehrenberg B, Gross E, Nitzan Y, Malik Z (1993) Electric depolarization of photosensitized cells: lipids vs. protein alterations. Biochim Biophys Acta 1151:257–264PubMedCrossRef

16.

Malik Z, Hanania J, Nitzan Y (1990) Bactericidal effects of photoactivated porphyrins—an alternative approach to antimicrobial drugs. J Photochem Photobiol B Biol 5:281–293CrossRef

17.

Spikes JD, Jori G (1987) Photodynamic therapy of tumours and other diseases using porphyrins. Lasers Med Sci 2:3–15CrossRef

18.

Wainwright M (1998) Photodynamic antimicrobial chemotherapy (PACT). J Antimicrob Chemother 42:13–28PubMedCrossRef

19.

Wilson M (1993) Photolysis of oral bacteria and its potential use in the treatment of caries and periodontal disease: a review. J Appl Bacteriol 75:299–306PubMed

20.

Komerik N, Wilson M, Poole S (2000) The effect of photodynamic action on two virulence factors of gram-negative bacteria. Photochem Photobiol 72:676–680PubMedCrossRef

21.

Packer S, Bhatti M, Burns T, Wilson M (2000) Inactivation of proteolytic enzymes from Porphyromonas gingivalis using light-activated agents. Lasers Med Sci 15:24–30CrossRef

22.

Fan KF, Hopper C, Speight P, Buonaccorsi G, Bown SC (1997) Photodynamic therapy using mTHPC for malignant disease in the oral cavity. Int J Cancer 73:25–32PubMedCrossRef

23.

Feres M, Haffajee AD, Goncalves C et al (1999) Systemic doxycycline administration in the treatment of periodontal infections. II. Effect on antibiotic resistance of subgingival species. J Clin Periodontol 26:784–792PubMed

24.

Grant WE, Buonaccorsi G, Speight P et al (1995) The effect of photodynamic therapy on the mechanical integrity of normal rabbit carotid arteries. Laryngoscope 105:867–871PubMedCrossRef

25.

Haas R, Baron M, Dortbudak O, Watzek G (2000) Lethal photosensitisation, autologous bone and e-PTFE membrane for the treatment of peri-implatitis: preliminary results. Int J Oral Maxillofac Implants 15:374–382PubMed

26.

KÖmerik N, Nakanishi H, MacRobert AJ (2003) In vivo killing of Porphyromonas gingivalis by toluidine blue-mediated photosensitization in an animal model. Antimicrob Agents Chemother 47:932–940PubMedCrossRef

27.

Chan Y, Lai CH (2003) Bactericidal effects of different laser wavelengths on periodontopathic germs in photodynamic therapy. Lasers Med Sci 18:51–55PubMedCrossRef

28.

Paulino TP, Ribeiro KF, Thedei G et al (2005) Use of hand held photopolymerizer to photoinactivate Streptococcus mutans. Arch Oral Biol 50:353–359PubMedCrossRef

29.

Williams JA, Pearson GJ, Colles MJ et al (2003) The effect of variable energy input from a novel light source on the photoactivated bactericidal action of toluidine blue O on Streptococcus mutans. Caries Res 37:190–193PubMedCrossRef

30.

Costerton JW, Stewart PS, Greenberg EP (1999) Bacterial biofilms: a common cause of persistent infections. Science 284:1318–1322PubMedCrossRef

31.

Matevski D, Weersink R, Tenenbaim HC (2003) Lethal photosensitization of periodontal pathogens by a red-filtered Xenon lamp in vitro. J Periodontal Res 38:428–435PubMed

32.

Romanova NA, Brovko LY, Moore L (2003) Assessment of photodynamic destruction of Escherichia coli O157:H7 and Listeria monocytogenes by using ATP bioluminescence. Appl Environ Microbiol 69(11):6393–6398PubMedCrossRef

33.

Demidova TN, Hamblin MR (2005) Effect of cell-Photosensitizer binding and cell density on microbial photoinactivation. Antimicrob Agents Chemother 49(6):2329–2335PubMedCrossRef

34.

Vaara M (1992) Agents that increase the permeability of the outer membrane. Microbiol Rev 56(3):395–411PubMed

35.

Capella M, Coelho AM, Menezes S (1996) Effect of glucose on photodynamic action of methylene blue in Escherichia coli cells. Photochem Photobiol 64:205–210PubMed

36.

Rovaldi CR, Pievsky A, Sole NA et al (2000) Photoactive porphyrin derivative with broad-spectrum activity against oral pathogens in vitro. Antimicrob Agents Chemother 44(12):3364–3367PubMedCrossRef

Titel: Toluidine blue-mediated photoinactivation of periodontal pathogens from supragingival plaques
verfasst von: Yanli Qin
Xiuling Luan
Liangjia Bi
Guopei He
XueFeng Bai
Chuannong Zhou
Zhiguo Zhang
Publikationsdatum: 01.01.2008
Verlag: Springer-Verlag
Erschienen in: Lasers in Medical Science / Ausgabe 1/2008
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-007-0454-x

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2008

Laser Florence 2007

Excimer laser in myocardial infarction: a comparison between STEMI patients with established Q-wave versus patients with non-STEMI (non-Q)

Differences in stress response between patients undergoing transurethral resection versus endoscopic laser ablation of the prostate for benign prostatic hyperplasia

A time resolved intense pulsed light spectral analysis system

Microstructural changes of enamel, dentin–enamel junction, and dentin induced by irradiating outer enamel surfaces with CO2 laser

Photodynamic inhibition of acetylcholinesterase after two-photon excitation of copper tetrasulfophthalocyanine